한방비만학회지 (Journal of Korean Medicine for Obesity Research)

  • 제16권2호
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  • Pages.85-91
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  • 2016
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  • 1976-9334(pISSN)
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  • 2288-1522(eISSN)
한방비만학회 (The Society of Korean Medicine for obesity Research)
권호 표지
DOI QR코드

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황금추출물이 과산화지질을 급여한 흰쥐의 지질대사, 산화반응 및 전염증성 Cytokine의 생산에 미치는 영향

Effects of Scutellariae Radix Extract on Lipid Metabolism, Oxidation and Production of Pro-Inflammatory Cytokines in Rats Fed Highly Oxidized Fat

  • 김성만 (가톨릭관동대학교 의과대학 의료경영학과) ;
  • 차윤엽 (상지대학교 한의과대학 한방재활의학과)
  • Kim, Sung-Man (Department of Medical Management, College of Medicine, Catholic Kwandong University) ;
  • Cha, Yun-Yeop (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Sangji University)
  • 투고 : 2016.11.17
  • 심사 : 2016.12.12
  • 발행 : 2016.12.30
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초록

목적: 본 연구는 황금추출물이 과산화지질을 장기간 급여한 흰쥐의 지질대사, 산화반응 및 전염증성 cytokine의 생산에 미치는 영향을 검토하기 위해 수행되었다. 방법: 10주 동안 흰쥐에게 과산화지질을 과량으로 급여하여 생체 내에 과잉의 지질을 축적시킨 후, 황금추출물을 6주간 급여하였다. 그 후, 각 처리군별로 지질대사, 산화반응 및 전염성 cytokine의 생산에 미치는 영향을 조사했다. 결과: 혈액 내 유리지방산, 총지질, 총콜레스테롤, low density lipoprotein-cholesterol과 간장 내 총지질, 총콜레스테롤이 황금추출물 처리군에서 하락했다. 혈액 및 간장의 thiobarbituric acid 농도가 황금추출물 처리군에서 하락했다. 간장 내 항산화효소들(glutathione peroxidase, liver catalase, liver superoxide dismutase)의 활성치가 황금처리군에서 상승하였다. 전염증성 cytokine(interleukin $[IL]-1{\beta}$, tumor necrosis $factor-{\alpha}$, IL-6)의 농도는 황금추출물 처리군에서 하락했다. 반면에 IL-10의 농도는 황금추출물 처리군에서 증가했다. 결론: 이상의 결과들을 종합해 보면, 황금 추출물은 과산화지질을 급여한 비만쥐의 지질대사, 항산화반응 및 항염증반응에 긍정적인 효과를 나타내었음을 인식시켜 준다.

Objectives: This study investigated the effects of Scutellariae Radix extract (SRE) on lipids metabolism, oxidation and the production of pro-inflammatory cytokines in rats fed highly oxidized fat. Methods: To induce obesity, male Sprague‐Dawley rats were fed a highly oxidized fat diet for 10 weeks. SRE at 100 mg/kg were administered orally to obesity-induced rats for 6 weeks, and their lipid metabolism, oxidation and production of pro-inflammatory cytokines were examined. Results: The concentrations of free fatty acid, triglyceride, total cholesterol, and low density lipoprotein-cholesterol in plasma decreased in SRE-treated groups, although the difference was not significant between control and SRE-treated groups, while that of high density lipoprotein-cholesterol significantly increased in SRE group. The concentrations of total cholesterol and triglyceride in the liver were tended to decrease in SRE-treated group. The concentrations of thiobarbituric acid in plasma and liver were lower in SRE group than in control group. The levels of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase in plasma were decreased in SRE group. Activities of glutathione peroxidase, superoxide dismutase, and catalase in liver were tended to increase in the SRE group. The plasma concentrations of interleukin $(IL)-1{\beta}$, tumor necrosis factor $(TNF)-{\alpha}$ and IL-6 were lower in SRE group than in control group, while that of IL-10 was higher. The liver concentrations of $IL-1{\beta}$, $TNF-{\alpha}$, and IL-6 were tended to decrease while that of IL-10 tended to increase in SRE group. Conclusions: Finally SRE could be used in the production of nutraceuticals for lowering lipids and exerting anti-oxidation and anti-inflammatory effects in obesity rats fed highly oxidized rat.

키워드

참고문헌

  1. Takayama S, Kahn CR, Kubo K, Foley JE. Alterations in insulin receptor autophosphorylation in insulin resistance: correlation with altered sensitivity to glucose transport and antilipolysis to insulin. J Clin Endocrinol Metab. 1988 ; 66(5) : 992-9. https://doi.org/10.1210/jcem-66-5-992
  2. Arner P, Pollare T, Lithell H, Livingston JN. Defective insulin receptor kinase in human skeletal muscle in obesity and type 2 (non-insulin-dependent) diabetes mellitus. Diabetologia. 1987 ; 30(6) : 437. https://doi.org/10.1007/BF00292549
  3. Kolterman OG, Insel J, Saekow M, Olefsky JM. Mechanism of insulin resistance in human obesity. J Clin Invest. 1980 ; 65(6) : 1272-84. https://doi.org/10.1172/JCI109790
  4. Saito W. Interaction between lipid peroxide formation and nutritional status. J JPN Soc Nutr Food Sci. 1988 ; 41(5) : 343-9. https://doi.org/10.4327/jsnfs.41.343
  5. Vergroeson AT. Physiological effects of dietary linoleic acid. Nutr Rev. 1997 ; 35(1) : 1-9. https://doi.org/10.1111/j.1753-4887.1977.tb06475.x
  6. Bidlack WR, Tappel AL. Damage to microsomal membrane by lipid peroxidation. Lipids. 1973 ; 8(4) : 177-8. https://doi.org/10.1007/BF02544631
  7. Hotamisligil GS, Shargill NS, Spiegelman BM. Adipose expression of tumor necrosis factor-${\alpha}$: direct role in obesity-linked insulin resistance. Science. 1993 ; 259(5091) : 87-91. https://doi.org/10.1126/science.7678183
  8. Ronald MK, Mary W. Obesity: impact on cardiovascular disease. Circulation. 1998 ; 98(8) : 1472-6. https://doi.org/10.1161/01.CIR.98.14.1472
  9. Robert HE, Ronald MK. American heart association call to action: obesity as a major risk factor for coronary heart disease. Circulation. 1998 ; 97(21) : 2099-100. https://doi.org/10.1161/01.CIR.97.21.2099
  10. Lee E, Choi MY, Oh HS. Effects of Powdered Siho (Bupleuri Radix) on serum and liver lipid composition and antioxidative capacity in rat fed high oxidized fat. Korean J Nutr. 2000 ; 33(5) : 502-6.
  11. Lee E. Effects of powdered pine needle (Pinus densiflora seib et Zucc.) on serum and liver lipid composition and antioxidative. J Korean Soc Food Sci Nutr. 2003 ; 32(6) : 926-30. https://doi.org/10.3746/jkfn.2003.32.6.926
  12. Kang YH, Ha TY, Moon KD. Effects of pine needle extracts on serum and liver lipid contents in rats fed high fat diet. J Korean Soc Food Nutr. 1996 ; 25(3) : 367-73.
  13. Ishikawa T, Suzukawa M. Effect of tea flavonoid supplementation on the susceptibility of low-density lipoprotein to oxidative modification. Am J Clin Nutr. 1997 ; 66(2) : 261-6. https://doi.org/10.1093/ajcn/66.2.261
  14. Kim HC. Textbook of herbal pharmacology. Seoul : Jipmundang. 2001 : 129-33.
  15. Middleton E Jr, Drzewiecki V. Naturally occuring flavonoids and human basophil histamine release. Int Archs Allergy Appl Immun. 1985 ; 77(1-2) : 155-7. https://doi.org/10.1159/000233771
  16. Wagner H. Antihepatotoxic flavonoids. In: Cody V, editors. Plant flavonoids in biology and medicine, biochemical, pharmacological and structure: activity relationships. New York : Alan R. Liss. 1986 : 545-58.
  17. Middleton E Jr, Harborne JB, Beretz A. Plant flavonoids in biology and medicine II. biochemical, cellular and medicineal properties. New York : Alan R. Liss. 1988 : 61-5.
  18. Middleton E Jr, Harborne JB. Plant flavonoids in biology and medicine, biochemical, pharmacological, structure-activity relationship. New York : Alan R. Liss. 1986 : 429-40.
  19. Kubo MY, Kimura T, Odani T, Tani T, Namba K. Studies on Scutellariae radix. II. The antibacterial substance. Planta Med. 1981 ; 43(2) : 194-201. https://doi.org/10.1055/s-2007-971499
  20. Kubo MH, Matusuda Y, Kimura H, Okuda M, Higashino T, Tani T, et al. Stidies on Scutellariae Radix. VII. Anti-arthrictic and anti-inflammatory action of methanolic extract and flavonoid components from Scutellariae Radix. Chem Pharm Bull. 1984 ; 32(7) : 2724-9. https://doi.org/10.1248/cpb.32.2724
  21. Tie H, Jin GB, Cho GB, Fumi S, Cyong JC. Evaluation of the anti-inflammatory effect of baicalein on dextran sulfate sodium-induced colitis in mice. Planta Med. 2002 ; 68(3) : 266-8. https://doi.org/10.1055/s-2002-23126
  22. Kaneda TK, Ueda N. Kasanka shishitsu shiken ho. Tokyo : Hirokawasyoten. 1987 : 58-9.
  23. Buege JA, Aust SD. Microsomal lipid peroxidation. In: Fleischer S, editors. Packer leds methods in enzymelogy. London : Academic Press. 1978 : 302-9.
  24. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxide in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979 ; 95(2) : 351-8. https://doi.org/10.1016/0003-2697(79)90738-3
  25. Levander OA, DeLoach DP, Morris VC, Moser PB. Platelet glutathione peroxidase activity as an index of selenium status in rats. J Nutr. 1983 ; 113(1) : 55-63. https://doi.org/10.1093/jn/113.1.55
  26. Flohe L, Becker R, Brigelius R, Lengfelder E, Otting F. Convenient assays for superoxide dismutase. CRC Handbook of free radicals and antioxidants in Biomedicine. Boca Raton, FL : CRC Press, Inc. 1992 : 287-93.
  27. Johnson LH, Hakan Borg LA. A spectro phtometric method for determination of catalase activity in small tissue samples. Anal Biochem. 1988 ; 174(1) : 331-6. https://doi.org/10.1016/0003-2697(88)90554-4

피인용 문헌

  1. 최근 10년간 한방비만학회지의 연구동향 분석: 2010-2019년 한방비만학회지 게재논문을 중심으로 vol.20, pp.2, 2016, https://doi.org/10.15429/jkomor.2020.20.2.149