Journal of The Korean Society of Clinical Toxicology (대한임상독성학회지)

Korean society of Clincal Toxicology (대한임상독성학회)
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In vitro Effects of Epigallocatechin Gallate on Sister Chromatid Exchange in the Lymphocytes Exposed to Glyphosate

글라이포세이트 노출로 인한 DNA손상에 대한 녹차의 예방적 효과

  • Park, Jung-Min (Department of Emergency Medicine, School of Medicine, Keimyung University, Dongsan Medical Center) ;
  • Choi, Woo-Ik (Department of Emergency Medicine, School of Medicine, Keimyung University, Dongsan Medical Center) ;
  • Jin, Sang-Chan (Department of Emergency Medicine, School of Medicine, Keimyung University, Dongsan Medical Center) ;
  • Lee, Jae-Ho (Department of Anatomy, College of Medicine, Keimyung University) ;
  • Choi, In-Jang (Department of Anatomy, College of Medicine, Keimyung University)
  • 박정민 (계명대학교 의과대학 동산의료원 응급의학교실) ;
  • 최우익 (계명대학교 의과대학 동산의료원 응급의학교실) ;
  • 진상찬 (계명대학교 의과대학 동산의료원 응급의학교실) ;
  • 이재호 (계명대학교 의과대학 해부학교실) ;
  • 최인장 (계명대학교 의과대학 해부학교실)
  • Received : 2016.08.22
  • Accepted : 2016.09.19
  • Published : 2016.12.31
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Abstract

Purpose: Green tea is known as a potent anti-oxidant, anti-carcinogen, and genetic protector. Glyphosate (N-phosphonomethyl glycine) is a widely used non-selective herbicide that causes DNA damage. The present study was conducted to investigate the protective effects of green tea in human blood lymphocytes exposed to glyphosate using the Sister Chromatid Exchange (SCE) frequency method. Methods: Peripheral blood was obtained from 10 volunteers and cultured through four different conditions. Four groups were divided into control, glyphosate only (300 ng/mL), glyphosate and low ($20{\mu}m$) concentrations of epigallocatechin gallate (EGCG) and glyphosate and high ($100{\mu}m$) concentrations of EGCG. Results: The glyphosate exposed groups had a higher mean SCE frequency ($10.33{\pm}2.50$) than the control group ($6.38{\pm}2.28$, p<0.001). The low concentrations of EGCG groups had a lower mean SCE frequency ($9.91{\pm}1.93$) than the glyphosate-only group, although this difference was not significant (p=0.219). However, the high concentration group ($9.49{\pm}1.85$) had a significantly lower SCE frequency than the glyphosate-only group (p=0.001). Conclusion: EGCG has a gene protective effect in human lymphocytes exposed to the genotoxicity of glyphosate in the case of high concentrations.

Keywords

References

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