The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Biological Control of Japanese Pine Sawyer, Monochamus alternatus (Coleoptera: Cerambycidae) using Korean Entomopathogenic Nematode Isolates

한국산 곤충병원성선충을 이용한 솔수염하늘소(Monochamus alternatus)의 생물적 방제

  • Received : 2016.10.15
  • Accepted : 2016.11.17
  • Published : 2016.12.31
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Abstract

Japanese pine sawyer (JPS), Monochamus alternatus is a vector insect of pine wood nematode, Bersaphlenchus xylophilus in Korea and Japanese pine forest. This study was to evaluate the possibility of biological control of JPS with Korean entomopathogenic nematodes (EPNs). Korean EPNs (Heterorhabditis sp. Gyeongsan, Steinernema carpocapsae GSN1, S. glaseri Dongrae and S. longicaudum Nonsan strain) were infested bate insect, Galleria mellonella in log of dead pine tree. Result showed that, S. carpocapsae GSN1 strain has the highest pathogenicity. Heterorhabditis sp. Gyeongsan and S. carpocapsae GSN1 had infested bate insect, which located in 7.5 cm depth of pine log. EPN has pathogenicity against larva and adult of JPS. Spray application of EPNs against adult of JPS, Heterorhabditis sp. Gyeongsan strain showed higher pathogenicity than S. carpocapsae GSN1 strain. EPN infested larva of JPS was detected on bark spray treatment with S. carpocapsae GSN1 strain in dead pine log that naturally infested larva of JPS however, no EPN infested JPS was detected on soaking of pine log with suspension of S. carpocapsae GSN1 strain. Though EPN had found with low efficacy against JPS but still possible to apply to control the boring insect pest as they can control log dwelling insect.

솔수염하늘소(Monochamus alternatus)는 우리나라와 일본의 소나무림에서 소나무재선충(Bursaphelenchus xylophilus)을 전파하는 매개충으로 알려져 있다. 본 연구는 곤충병원성선충을 이용하여 솔수염하늘소의 생물적 방제 가능성을 알아보기 위하여 수행하였다. 실험에 이용한 한국산 곤충병원성선충 4종(Heterorhabditis sp. Gyeongsan, Steinernema carpocapsae GSN1, S. glaseri Dongrae, S. longicaudum Nonsan 계통)은 소나무 고사목 내의 미끼곤충인 꿀벌부채명나방 유충을 치사시켰는데 S. carpocapsae GSN1의 병원성이 가장 높았다. 두 선충 모두 7.5 cm 깊이에 있는 꿀벌부채명나방을 치사시켰다. 곤충병원성선충은 솔수염하늘소 유충과 성충 모두에 병원성을 보였는데 성충에 대한 분무처리 시 S. carpocapsae GSN1 계통보다 Heterorhabditis sp. Gyeongsan 계통의 병원성이 높았다. 솔수염하늘소 유충이 서식하고 있는 소나무 벌채목에 대한 S. carpocapsae GSN1 계통의 수피면 분무처리와 벌채목 수침 처리 시 분무처리에서만 선충에 감염 된 유충이 확인되었다. 곤충병원성선충은 고사목 수체 내 솔수염하늘소 유충 방제의 실용성은 낮았지만 수체 내 해충에 대한 방제 가능성은 있어 추후 천공성 해충의 방제에 활용 가능성이 있을 것으로 생각된다.

Keywords

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