생명과학회지 (Journal of Life Science)

  • 제26권11호
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  • Pages.1320-1329
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  • 2016
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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사람의 다양한 조직에서 기원하는 암세포 및 정상세포에 대한 penta-O-galloyl-β-D-glucose의 세포독성 효과

Differential Cytotoxicity of Penta-O-galloyl-β-D-glucose in Human Cancer and Normal Cell Lines of Various Origins

  • 이현정 (경남과학고등학교) ;
  • 김민경 (경남과학고등학교) ;
  • 이송영 (경남과학고등학교) ;
  • 송민혁 (경남과학고등학교) ;
  • 김윤동 (경남과학고등학교) ;
  • 하정숙 (경상대학교 사범대학 생물교육과) ;
  • 정계준 (경상대학교 사범대학 생물교육과) ;
  • 노규진 (경상대학교 수의과대학 수의학과) ;
  • 전병균 (경상대학교 사범대학 생물교육과)
  • Lee, Hyeon-Jeong (Gyeongnam Science High School) ;
  • Kim, Min-Gyeong (Gyeongnam Science High School) ;
  • Lee, Song-Yeong (Gyeongnam Science High School) ;
  • Song, Min-Hyock (Gyeongnam Science High School) ;
  • Kim, Yoon-Dong (Gyeongnam Science High School) ;
  • Ha, Jeong-Sook (Department of Biology Education, College of Education, Gyeongsang National University) ;
  • Jeong, Gie-Joon (Department of Biology Education, College of Education, Gyeongsang National University) ;
  • Rho, Gyu-Jin (OBS/Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Jeon, Byeong-Gyun (Department of Biology Education, College of Education, Gyeongsang National University)
  • 투고 : 2016.06.08
  • 심사 : 2016.09.22
  • 발행 : 2016.11.30
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초록

본 연구는 다당체의 한 종류인 penta-O-galloyl-${\beta}$-D-glucose (PGG)가 사람의 여러 조직에서 기원하는 여러 암세포주(A-549, MDA-MB-231, U87-MG, MCF-7 및 PANC-1), 정상 MRC-5 태아 섬유아세포 그리고 사랑니에서 유래한 간엽줄기세포(DPSCs)에 미치는 세포독성 효과를 조사하였다. $IC_{50}$값은 다른 세포주에 비해 높은 증식률을 나타내는 A-549 및 MDA-MB-231 암세포주에서 유의적으로 낮게 관찰되었다. 10 uM의 PGG가 포함된 배양액에서 세포를 7일 동안 배양한 결과, 세포배가시간은 모든 세포주에서 유의적으로 늘어났고, 세포배가시간과 $IC_{50}$값의 관계를 조사한 결과, 세포배가시간이 늘어남에 따라 $IC_{50}$값은 비례적으로 증가됨을 증명하였다. 또한, 10 uM의 PGG로 처리된 세포주들은 노화와 관련된 ${\beta}-galactosidase$의 활성도가 높게 관찰되었다. 특히, telomerase 활성도는 A-549 및 MDA-MB-231 암세포주에서 다른 세포주에 비하여 현저히 감소하는 것을 관찰하였다. 이러한 결과를 바탕으로 PGG는 높은 증식률을 보이는 암세포주에서 높은 세포독성효과를 나타내어 잠재적인 항암물질임을 증명하였다.

The present study examined the cytotoxic effects of 1, 2, 3, 4, 6-penta-O-galloyl-${\beta}$-D-glucose (PGG), known as the pentahydroxy gallic acid ester of glucose, in the various human cancer cell lines (A-549, MDA-MB-231, U87-MG, MCF-7 and PANC-1), normal MRC-5 fetal fibroblasts, and dental papilla tissue- derived mesenchymal stem cells (DPSCs). Significantly (p<0.05) lower half maximal inhibitory concentration ($IC_{50}$) values were observed in the A-549 and MDA-MB-231 cells showing a high proliferation capacity, compared with other cancer and normal cell lines with a relatively low proliferation capacity. The population doubling time (PDT) was significantly (p<0.05) higher in the $10{\mu}M$ PGG-treated cell lines than those of untreated control cell lines. The present study demonstrated that the $IC_{50}$ value increases proportionally to the extending PDT. A high cell number with senescence-associated ${\beta}-galactosidase$ activity was also observed in the $10{\mu}M$ PGG-treated cells compared with those of untreated control cells. Moreover, the level of telomerase activity was significantly (p<0.05) decreased with $10{\mu}M$ PGG treatment, especially in A-549 and MDA-MB-231 cells showing a high proliferation capacity. Based on these observations, PGG could serve as a potent agent for cancer chemotherapy, as its treatment was more effective in cells with a high proliferation capacity.

키워드

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