Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Differential Cytotoxicity of Penta-O-galloyl-β-D-glucose in Human Cancer and Normal Cell Lines of Various Origins

사람의 다양한 조직에서 기원하는 암세포 및 정상세포에 대한 penta-O-galloyl-β-D-glucose의 세포독성 효과

  • Lee, Hyeon-Jeong (Gyeongnam Science High School) ;
  • Kim, Min-Gyeong (Gyeongnam Science High School) ;
  • Lee, Song-Yeong (Gyeongnam Science High School) ;
  • Song, Min-Hyock (Gyeongnam Science High School) ;
  • Kim, Yoon-Dong (Gyeongnam Science High School) ;
  • Ha, Jeong-Sook (Department of Biology Education, College of Education, Gyeongsang National University) ;
  • Jeong, Gie-Joon (Department of Biology Education, College of Education, Gyeongsang National University) ;
  • Rho, Gyu-Jin (OBS/Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Jeon, Byeong-Gyun (Department of Biology Education, College of Education, Gyeongsang National University)
  • 이현정 (경남과학고등학교) ;
  • 김민경 (경남과학고등학교) ;
  • 이송영 (경남과학고등학교) ;
  • 송민혁 (경남과학고등학교) ;
  • 김윤동 (경남과학고등학교) ;
  • 하정숙 (경상대학교 사범대학 생물교육과) ;
  • 정계준 (경상대학교 사범대학 생물교육과) ;
  • 노규진 (경상대학교 수의과대학 수의학과) ;
  • 전병균 (경상대학교 사범대학 생물교육과)
  • Received : 2016.06.08
  • Accepted : 2016.09.22
  • Published : 2016.11.30
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Abstract

The present study examined the cytotoxic effects of 1, 2, 3, 4, 6-penta-O-galloyl-${\beta}$-D-glucose (PGG), known as the pentahydroxy gallic acid ester of glucose, in the various human cancer cell lines (A-549, MDA-MB-231, U87-MG, MCF-7 and PANC-1), normal MRC-5 fetal fibroblasts, and dental papilla tissue- derived mesenchymal stem cells (DPSCs). Significantly (p<0.05) lower half maximal inhibitory concentration ($IC_{50}$) values were observed in the A-549 and MDA-MB-231 cells showing a high proliferation capacity, compared with other cancer and normal cell lines with a relatively low proliferation capacity. The population doubling time (PDT) was significantly (p<0.05) higher in the $10{\mu}M$ PGG-treated cell lines than those of untreated control cell lines. The present study demonstrated that the $IC_{50}$ value increases proportionally to the extending PDT. A high cell number with senescence-associated ${\beta}-galactosidase$ activity was also observed in the $10{\mu}M$ PGG-treated cells compared with those of untreated control cells. Moreover, the level of telomerase activity was significantly (p<0.05) decreased with $10{\mu}M$ PGG treatment, especially in A-549 and MDA-MB-231 cells showing a high proliferation capacity. Based on these observations, PGG could serve as a potent agent for cancer chemotherapy, as its treatment was more effective in cells with a high proliferation capacity.

본 연구는 다당체의 한 종류인 penta-O-galloyl-${\beta}$-D-glucose (PGG)가 사람의 여러 조직에서 기원하는 여러 암세포주(A-549, MDA-MB-231, U87-MG, MCF-7 및 PANC-1), 정상 MRC-5 태아 섬유아세포 그리고 사랑니에서 유래한 간엽줄기세포(DPSCs)에 미치는 세포독성 효과를 조사하였다. $IC_{50}$값은 다른 세포주에 비해 높은 증식률을 나타내는 A-549 및 MDA-MB-231 암세포주에서 유의적으로 낮게 관찰되었다. 10 uM의 PGG가 포함된 배양액에서 세포를 7일 동안 배양한 결과, 세포배가시간은 모든 세포주에서 유의적으로 늘어났고, 세포배가시간과 $IC_{50}$값의 관계를 조사한 결과, 세포배가시간이 늘어남에 따라 $IC_{50}$값은 비례적으로 증가됨을 증명하였다. 또한, 10 uM의 PGG로 처리된 세포주들은 노화와 관련된 ${\beta}-galactosidase$의 활성도가 높게 관찰되었다. 특히, telomerase 활성도는 A-549 및 MDA-MB-231 암세포주에서 다른 세포주에 비하여 현저히 감소하는 것을 관찰하였다. 이러한 결과를 바탕으로 PGG는 높은 증식률을 보이는 암세포주에서 높은 세포독성효과를 나타내어 잠재적인 항암물질임을 증명하였다.

Keywords

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