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건대추 열수추출물을 이용한 대추와인 발효중의 이화학 및 항산화적 특성 변화

Changes in the Physicochemical and Antioxidant Characteristics during the Fermentation of Jujube Wine Using Hot Water Extract of Dried Jujube

  • Eom, In-Ju (Department of Food Engineering, Daegu University) ;
  • Choi, Jung-In (Department of Food Engineering, Daegu University) ;
  • Kim, In-Ho (Department of Food Engineering, Daegu University) ;
  • Kim, Tae-Hoon (Department of Food Engineering, Daegu University) ;
  • Kim, Seong-Ho (Department of Food Engineering, Daegu University)
  • 투고 : 2016.06.29
  • 심사 : 2016.08.17
  • 발행 : 2016.11.30

초록

본 연구는 대추 열수 추출액을 이용한 대추 와인의 발효중 이화학적, 항산화적 특성 변화 및 최적발효조건을 조사하였다. 실험에 사용된 대추 열수 추출물의 이화학적 특성으로는 pH 5.05, 산도 0.01% 및 농도 $6.5^{\circ}Brix$이었다. 대추 와인의 발효를 위한 최적의 발효 균주가 최종 선별되었고 최종 선정된 1균주는 26S rRNA gene sequencing과 GenBank DB의 유사성 분석에 의하여 Saccharomyces cerevisiae로 동정 되었다. 대추 와인 발효를 위한 추출액의 초기 농도 $15^{\circ}Brix$에서 알코올 함량은 13%로 가장 높았고 잔당 함량은 가장 낮은 값을 보였다. 대추 와인발효 기간중의 알코올 함량은 3일째부터 급격한 증가가 있었고 발효 6일 이후는 유의적인 차이가 없었으며, 잔당 함량은 알코올 함량의 증가와 더불어 유의적으로 감소하였다. 발효 12일째 대추 와인의 pH는 4.34, 산도는 0.29%, 알코올 함량은 12.8%, 당도는 $8.70^{\circ}Brix$로 조사되었다. 대추 와인의 유기산 함량은 malic acid의 경우, 발효가 진행됨에 따라 유의적으로 감소하는 경향을 보였고 succinic acid와 lactic acid는 유의적으로 증가하는 것으로 나타났다. 대추 와인의 항산화능 특성 중 ABTS는 45.80%, DPPH은 61.89%, 아질산염 소거활성능은 91.95%이었고, total polyphenol은 3.69 mg/ml의 값을 보였다. 대추 와인의 관능적 평가에 의한 소비자 기호도에서 색과 전체적인 기호도면에서 평균 이상으로 평가되었다.

In the study, we investigated the optimum fermentation conditions as well as changes of physicochemical and antioxidant characteristics during the fermentation of jujube wine. The physicochemical characteristics of the jujube hot water extracts used in this study were a pH of 5.05, 0.01% acidity, and $6.5^{\circ}Brix$ concentration. For jujube wine fermentation, the optimal fermentation strain was selected among the isolated strains and the final chosen strain was identified as Saccharomyces cerevisiae, based on the 26S rRNA gene sequencing and similarity searching in GenBank DB. The jujube wine fermented with an initial $15^{\circ}Brix$ concentration of jujube extracts showed a maximum alcohol content of 13% and lower residual sugar concentration. Alcohol content during the jujube wine fermentation was increased after 3 days of fermentation, and no significantly difference after 6 days was found. The residual sugar concentration during the fermentation periods was significantly decreased with increasing alcohol content. The jujube wine properties at 12 days of fermentation were as follows: a pH of 4.34, acidity of 0.29%, alcohol content of 12.8%, and a residual sugar concentration of $8.70^{\circ}Brix$. The malic acid content in the organic acid of fermented jujube wine was significantly decreased during the fermentation proceeding, whereas the succinic acid and lactic acid contents were significantly increased. Antioxidant characteristics of the fermented jujube wine were appeared ABTS radical scavenging activity 45.80%, DPPH radical scavenging activity 61.89%, nitrite scavenging activity 91.95% and total polyphenol compound 3.69 mg/ml. In terms of consumer liking of the jujube wine by sensory evaluation, the color and overall acceptability of jujube wine were evaluated as more than average.

키워드

참고문헌

  1. Ahn, J. B., Choi, S. H., Kim, H. R., Park, M. L., Lee, S. H. and Kim, D. S. 2012. Development of teriyaki sauce added with jujube concentrate (Ziziphus jujube Miller) extracts. Kor. J. Culin. Res. 18, 239-251.
  2. AOAC. Official Method of Analysis. 18th ed. 2005. The Association of official Analytical Chemists, Washington DC, USA.
  3. Auh, M. S., Kim, Y. S., Ahn, S. J., Ahn, J. B. and Kim, K. Y. 2012. Comparison of property changes of black jujube and Zizyphus jujube extracts during lactic acid fermentation. J. Kor. Soc. Food Sci. Nutr. 41, 1346-1355. https://doi.org/10.3746/jkfn.2012.41.10.1346
  4. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1202.
  5. Cyong, J. C. and Hanabusa, K. 1980. Cyclic adenosine monophosphate in fruits of Zizyphus jujuba. Phytochemistry 19, 2747-2748. https://doi.org/10.1016/S0031-9422(00)83955-2
  6. Duval, B. and Shetty, K. 2001. The stimulation of phenolics and antioxidant activity in pea (Pisum sativum) elicited by genetically transformed anise root extract. J. Food Biochem. 25, 361-377. https://doi.org/10.1111/j.1745-4514.2001.tb00746.x
  7. Joo, O. K., Kang, S. T., Jeong, C. H., Lim, J. W., Park, Y. G. and Cho, K. M. 2011. Manufacturing of enhances antioxidative wine using a ripe Daebong persimmmon. J. Appl. Biol. Chem. 54, 126-134. https://doi.org/10.3839/jabc.2011.022
  8. Jung, G. T., Ju, I. O., Ryn, J., Choi, J. S. and Choi, Y. G. 2003. Studies on manufacture of wine using apricot. Kor. J. Food Preserv. 10, 493-497.
  9. Kang, T. S., Woo, K. S., Lee, J. S. and Jeong, H. S. 2006. Fermentation characteristics of wine using fresh jujube. Food Eng. Prog. 10, 164-171
  10. Kato, H., Lee, I. E., Chuyen, N. V., Kim, S. B. and Hayase, F. 1987. Inhibitory of nitrosamine formation by nondilyzable melanoidins. Agric. Biol. Chem. 51, 1333-1338.
  11. Kim, A. N., Park, S. H. and Jung, H. A. 2014. Antioxidant activity of jujube and curd yogurt addition to jujube. Kor. J. Food Nutr. 3, 331-338.
  12. Kim, G. H., Kim, J. H. and Yang, J. Y. 2014. Change in flavor components of black-fermented garlic wine according to the type of chips during the manufacturing process. J. Food Hyg. Safety 29, 73-77. https://doi.org/10.13103/JFHS.2014.29.1.073
  13. Kim, H. K. and Joo, K. J. 2005. Antioxidative capacity and total phenolic compounds of methanol extract from Zizyphus jujuba. J. Kor. Soc. Food Sci. Nutr. 34, 750-754. https://doi.org/10.3746/jkfn.2005.34.6.750
  14. Kim, I. H., Jeong, C. H., Park, S. J. and Shim, K. H. 2011. Nutritional components and antioxidative activities of jujube (Zizyphus jujuba) fruit and leaf. Kor. J. Food Preserv. 18, 341-348. https://doi.org/10.11002/kjfp.2011.18.3.341
  15. Kim, J. H., Lee, D. H., Choi, S. Y. and Lee, J. S. 2002. Characterization of physiological functionalities in Korean traditional liquors. Kor. J. Food Sci. Technol. 34, 118-122.
  16. Kim, O. M., Jo, Y. J. and Jeong, Y. J. 2013. Development plan of wine sommelier education in Korea. Food Industry and Nutrition 18, 17-24.
  17. Kim, Y. S., Jeong, D. Y. and Shin, A. H. 2008. Optimum fermentation conditions and fermentation characteristics of mulberry (Morus alba) wine. Kor. J. Food Sci. Technol. 40, 63-69.
  18. Lee, J. M., Kim, S. K. and Lee, G. D. 2003. Monitoring on alcohol fermentation characteristics of strawberry. J. Kor. Soc. Food Sci. Nutr. 32, 679-683. https://doi.org/10.3746/jkfn.2003.32.5.679
  19. Lee, S., Moon, H. K., Lee, S. W., Moon, J. N., Kim, D. H. and Kim, G. Y. 2014. Monitoring of wine quality by using environmentally friendly tomato concentrate and commercial wines. Kor. J. Food Cult. 29, 278-285. https://doi.org/10.7318/KJFC/2014.29.3.278
  20. Mo, H. W., Jeong, J. S., Choi, S. W. and Choi, K. H. 2012. Preparation of wine using wild yeast form dried omija and optimal nutritional requirements for alcoholic fermentation. J. Kor. Soc. Food Sci. Nutr. 41, 254-260. https://doi.org/10.3746/jkfn.2012.41.2.254
  21. National Tax Service Technical Service Institute. 1999. Alcoholic beverage analysis rule. Sejung Pub Co, Seoul, Korea, p 196.
  22. Park, C. S. and Kim, M. L. 2010. Preparation and characterization of watermelon wine. Kor. J. Food Preserv. 17, 547-554.
  23. Park, H. S. 2011. The antioxidant and nitrite scavenging activity of wild grape (Vitis coignetiea) wine. J. East Asian Soc. Diet. life 21, 68-73.
  24. Peryam, D. R. and Giradot, N. F. 1952. Advanced taste test method. Food Eng. 24, 58-61.
  25. Rhee, S. J., Lee, C. Y. J., Kim, K. K. and Lee, C. H. 2003. Comparison of the traditional (Samhaeju) and industrial (Chongju) rice wine brewing in Korea. Kor. Food Sci. Biotechnol. 12, 242-247.
  26. Ribereau-Gayon, P., Glories, Y., Maujean, A. and Dubourdieu, D. 2000. Phenolic compounds: The chemistry of wine stabilization and treatments. In Handbook of enology. Ribereau-Gayon P. ed. John Wiley & Sons Ltd., Chichester, England. Vol. 2, p 129-157.
  27. Roberta, R., Nicoletta, P., Anna, P., Anath, P., Min, Y. and Catherine, R. E. 1999. Antioxidant activity applying an improved ABTS radical cation, decolorization assay. Free Radic. Biol. Med. 26, 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  28. Sadler, G. O. 1994. Titratable acidity. In: Introduction to the chemical analysis of foods, pp 83-94, Nielson S. S. (ed). James and Bartlett Publisher, London, UK.
  29. Saitou, N. and Nei, M. 1987. The neighbor joining-methods: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.
  30. Seo, J. H., Oh, S. H. and Kim, M. R. 2002, Quality characteristics and food and nutrition specialists' opinion on jujube teas, Kor. J. Soc. Food Cookery Sci. 18, 670-676.
  31. Seo, S. H., Yoo, S. A., Kang, B. S. and Son, H. S. 2014. Quality characteristics of Korean black raspberry Bokbunja wines produced using different amounts of water in the fermentation process. Kor. J. Food Sci. Technol. 46, 33-38. https://doi.org/10.9721/KJFST.2014.46.1.33
  32. Seo, S. H., Yoo, S. A., Park, S. E. and Son, H. S. 2014. Effectiveness of yeast nutrients on stuck fermentation of blueberry wine. Kor. J. Food Sci. Technol. 46, 143-147. https://doi.org/10.9721/KJFST.2014.46.2.143
  33. Stone, H. and Sidel, J. L. 1985. Sensory evaluation practices. 2nd ed. Academic press Inc., New York, USA. p 45.
  34. Tamura. K., Dudley, J., Nei, M. and Kumar, S. 2007. MEGA4, Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Bio.l Evol. 24, 1596-1599. https://doi.org/10.1093/molbev/msm092
  35. Woo, S. M., Lee, M. H., Seo, J. H., Kim, Y. S., Choi, H. E., Choi, I. W. and Jeong, Y. J. 2007. Quality characteristics of kiwi wine on alcohol fermentation strains. J. Kor. Soc. Food Sci. Nutr. 36, 800-806. https://doi.org/10.3746/jkfn.2007.36.6.800
  36. Yeates, C., Gillings, M. R., Davison, A. D., Altavilla, N. and Veal, D. A. 1998. Methods for microbial DNA extraction from soil for PCR amplification. Biol. Proced. Online 1, 40-47. https://doi.org/10.1251/bpo6