Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Optimizing Maillard Reaction for Development of Natural Seasoning Source Using Oyster Hydrolysate

굴 가수분해물을 이용한 천연조미소스 개발을 위한 마이얄 반응의 최적화

  • Ryu, Tae-hyun (Department of Food Science & Technology, Pukyoung National University) ;
  • Kim, Jin-hee (Department of Food Science & Technology, Pukyoung National University) ;
  • Shin, Jiyoung (Department of Food Science & Technology, Pukyoung National University) ;
  • Kim, Hyeon-jeong (Department of Food Science & Technology, Pukyoung National University) ;
  • Yang, Ji-young (Department of Food Science & Technology, Pukyoung National University)
  • Received : 2016.04.18
  • Accepted : 2016.08.02
  • Published : 2016.11.30
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Abstract

The oyster is called "milk of sea" which is abundant in taurine, glycogen, cellenium. It could be used in making natural source. Recently, consumers have more interest in natural source because of their diverse preference and its special taste. The goal of this study is to optimize maillard reaction condition for manufacturing natural seasoning using oyster and oyster cooking drip hydrolysate. The result was judged by browning degree and pyrazine, which is flavor components when food heating. Hydrolysate and sugar react according primarily to type of sugar - glucose, xylose and fructose. Xylose was selected as best sugar of browning degree. In the case of sugar contents, all conditions over 1% of sugar contents are almost same. Therefore, the lowest 1% of sugar was selected as appropriate condition. According to the reaction with different temperature, browning degree and pyrazine contents had been increased over $60^{\circ}C$, but the product at $120^{\circ}C$had off-flavored. So, $100^{\circ}C$ is the best condition for the browning reaction. And in accordance with different reaction time, after 6 hours, there was no change in pyrazine and browning reaction. Therefore, to manufacture natural seasoning source, it is optimal to react xylose for maillard reaction at $100^{\circ}C$ for 6 hr with hydrolysate of oyster and oyster cooking drip.

굴은 "바다의 우유"라고 불릴 정도로 영양분이 풍부하고, 균형이 잡혀 있다. 또한 타우린, 글리코겐, 셀레늄과 같은 성분이 풍부하다고 알려져 있다. 또한 이러한 영양학적 특징만을 가지고 있는 것이 아니라 식품 산업에서는 천연소스로 사용되어지기도 한다. 최근 다양한 선호도와 특별한 맛 때문에 소비자들의 천연소스에 대한 선호도가 높아졌다. 이 논문은 굴과 굴 가공 부산물인 굴 자숙액을 이용한 천연소스 개발을 위하여 소스 제조에 필요한 마이얄 반응의 최적 조건을 설정하고자하였다. 최적화를 위한 결과값은 갈색도와 열을 가하면 생성되는 향미성분인 pyrazine으로 판단한다. Protamex를 이용하여 굴과 굴 자숙액을 각각 6시간, 2시간 가수분해한 가수분해물과 당은 주로 glucose, xylose, fructose와 반응시켰고, xylose가 갈색화에 가장 적합하였다. 당함량은 1% 이상에서 반응하여도 거의 동일하였으며, 경제적으로 판단한 결과, 최적 농도는 가장 낮은 농도의 1%를 선택되었다. 또한 온도를 달리하여 반응을 시켰을 때, 갈변도는 $60^{\circ}C$ 이상에서 현저히 증가하는 경향을 보였고, 전반적으로는 갈변도와 pyrazine 모두가 온도가 상승해감에 따라 증가하였다. $120^{\circ}C$에서 가장 높은 수치를 보였지만, 이취가 발생하여 식품으로서는 부적합하였다. 따라서 $100^{\circ}C$에서 반응하는 것이 가장 적합하였다. 반응시간을 달리하여 측정한 결과, 6시간 까지는 상승을 하였으나, 6시간 이상에서 pyrazine과 갈색도의 변화가 없었다. 따라서 천연소스를 제조하기 위해 굴과 굴 자숙액 가수분해물을 이용해 $100^{\circ}C$, 6시간 동안 xylose로 마이알 반응을 시키는 것이 가장 적합하다고 판단하였다. 이러한 부산물을 이용하여 소스로 제조함으로서 산업적 이용이 가능할 것으로 판단된다.

Keywords

References

  1. Ames, J. M. 1998. Applications of the maillard reaction in the food industry. Food Chem. 72, 119-125.
  2. Chung, I. K., Kim, H. S., Kang, K. T., Choi, J. D., Heu, M. S. and Kim, J. S. 2006. Preparation and characterization of enzymatic oyster hydrolysates-added yogurt. J. Kor. Soc. Food Sci. Nutr. 35, 926-934. https://doi.org/10.3746/jkfn.2006.35.7.926
  3. Chung, I. K., Kim, H. S., Kang, K. T., Choi, Y. J., Choi J. D., Heu, M. S. and Kim, J. S. 2006. Preparation and functional properties of enzymatic oyster hydolysates. J. Kor. Soc. Food Sci. Nutr. 35, 919-925. https://doi.org/10.3746/jkfn.2006.35.7.919
  4. National Fisheries Research and Development Institute. 2012. Standard Manual of Pacific Oyster Hanging culture. 2-7.
  5. Je, J. Y., Park, P. J., Jung, W. K. and Kim, S. K. 2005. Amino acid changes in fermented oyster (Crassostrea Gigas) sauce with different fermentation periods. Food Chem. 91, 15-18. https://doi.org/10.1016/j.foodchem.2004.05.061
  6. Jeon, Y. H., Kim, J. K. and Kim, W. J. 1986. Effect of Temperature, pH and Sugars on Kinetic Property of Maillard reaction. Kor. J. Food Sci. Technol. 18, 55-60.
  7. Kim, C. Y., Pyeun, J. H. and Nam, J. N. 1981. Decomposition of glycogen and protein in pickled oyster during fermentation with salt. J. Kor. Fish. Soc. 14, 66-71.
  8. Kim, J. S. and Heu, M. S. 2001. Preparation of instant powdered soup using canned oyster processing waste water and its characteristics. J. Kor. Fish. Soc. 34, 285-290.
  9. Kim, J. S., Heu, M. S. and Yeum, D. M. 2001b. Component characteristics of canned oyster processing waste water as a food resource. J. Kor. Soc. Food Sci. Nutr. 20, 299-306.
  10. Kim, S. K., Byun, H. G., Jeon, Y. J., Joo, D. S. and Kim, J. B. 1999. Development of natural seasoning using desalinated tuna boiled extract. J. Kor. Fish. Soc. 32, 75-82.
  11. Kim, Y. S., Moon, J. H., Kim, M. H., Choi, H. D. and Park, Y. K. 2009. Physiochemical properties and antioxidant activities of maillard reaction products from defatted hydrolyzed soybean protein with various sugars. J. Kor. Soc. Food Sci. Nutr. 38, 62-69. https://doi.org/10.3746/jkfn.2009.38.1.062
  12. Ko, S. N., Yoon, S. H., Yoon, S. K. and Kim, W. J. 1997. Developed of meat-like flavor by maillard reaction of model system with amino acids and sugars. J. Kor. Food Sci. Technol. 29, 827-838.
  13. Lertittikul, W., S. and Benjakul, M. Tanaka. 2007. Characteristics and antioxidative activity of maillard reaction products from a porcine plasma protein-glucose model system as influenced by pH. Food Chem. 100, 669-677. https://doi.org/10.1016/j.foodchem.2005.09.085
  14. Li, Y., Lu, F., Luo, C. R., Chen, Z. X., Mao, J., Shoemaker, C. and Zhong, F. 2009. Functional properties of the maillard reaction products of rice protein with sugar. Food Chem. 117, 69-74. https://doi.org/10.1016/j.foodchem.2009.03.078
  15. Morales, F. J. and Jimenez-Perez, S. 2001. Free radical scavenging capacity of maillard reaction products as related to colour and floroescence. Food Chem. 72, 119-125. https://doi.org/10.1016/S0308-8146(00)00239-9
  16. Oh, H. S., Kim, J. S., Kim, H. S., Jee, S. J., Lee, J. H., Chung, I. K., Kang, K. T. and Heu, M. S. 2007. Improvement on the quality and functionality of skipjack tuna cooking drip using commercial enzymes, J. Kor. Soc. Food Sci. Nutr. 36, 881-888. https://doi.org/10.3746/jkfn.2007.36.7.881
  17. Ryu, S. Y., Roh, H. J., Noh, B. S., Kim, S. Y., Oh, D. K., Lee, W. J., Yoon, J. R. and Kim, S. S. 2003. Effects of various sugars including tagatose and their molar concentrations on the maillard browning reaction. J. Kor. Food Sci. Technol. 35, 898-904.
  18. Shiau, C. Y. and Chai, T. 1990. Characterization of oyster shucking liquid wastes and their utilization as oyster soup. J. Food Sci. 55, 374-378. https://doi.org/10.1111/j.1365-2621.1990.tb06767.x
  19. Shon, S. H., Cho, I. H. and Kim, Y. S. 2004. Comparison of pyrazines formed in chicken by-products hydrolyzed by enzymes. J. Kor. Soc. Food Cookery Sci. 20, 265-270.
  20. Umayaparvathi, S., Meenakshi, S., Vimalraj, V., Arumugam, M., Sivagami, G. and Balasubramanian, T. 2014. Antioxidant activity and anticancer effect of bioactive peptide from enzymatic hydrolysate of oyster (Saccostrea Cucullata). Biomedicine Preventive Nutr. 4, 343-353. https://doi.org/10.1016/j.bionut.2014.04.006
  21. Yoon, H. D., Byun, H. S., Chun, S. J., Kim, S. B. and Park, Y. H. 1986. Lipid composition of oyster, Arkshell and Seamessel. J. Kor. Fish Soc. 19, 321-326.
  22. Yoon, S. H., Lee, J. K., Nam, H. S. and Lee, H. J. 1994. Formation of meatlike flavors by maillard reaction using hydrolyzed vegetable protein (HVP). Kor. J. Food Sci. Technol. 26, 781-786.
  23. Suzuki, Y., Choi, K. J., Uchida, K., Ko, S. R., Sohn, H. J. and Park J. D. 2004. Arginyl-fructosyl-glucose and arginylfructose, compounds related to browning reaction in the model system of steaming and heat-drying processes for the preparation of Red Ginseng. J. Ginseng Res. 28, 143-148. https://doi.org/10.5142/JGR.2004.28.3.143