Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Polymorphisms in the 3' Untranslated Region of the Porcine Mitochondrial calcium uptake 1 (MICU1) Gene on Meat Quality Traits

돼지 mitochondrial calcium uptake 1 (MICU1) 유전자의 3'UTR 내 SNP가 육질에 미치는 영향

  • Jee, Yae-Sol (Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration) ;
  • Cho, Eun-Seok (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Jeon, Hyeon-Jeong (Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Si-Woo (Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lim, Kyu-Sang (Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Tae-Hun (Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Kyung-Tai (Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration)
  • 지예솔 (농촌진흥청 국립축산과학원 동물유전체과) ;
  • 조은석 (농촌진흥청 국립축산과학원 양돈과) ;
  • 전현정 (농촌진흥청 국립축산과학원 동물유전체과) ;
  • 이시우 (농촌진흥청 국립축산과학원 동물유전체과) ;
  • 임규상 (농촌진흥청 국립축산과학원 동물유전체과) ;
  • 김태헌 (농촌진흥청 국립축산과학원 동물유전체과) ;
  • 이경태 (농촌진흥청 국립축산과학원 동물유전체과)
  • Received : 2016.09.13
  • Accepted : 2016.10.05
  • Published : 2016.11.30
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Abstract

Mitochondrial calcium uptake 1 (MICU1) including two canonical EF hands, located in the mitochondrial inner membrane, is known to play a crucial role in the calcium uptake in mitochondria. Mitochondrial calcium uptake in muscular cells is related to post mortem shortening by calcium release from muscles. Therefore, the porcine MICU1 gene has been estimated as a genetic candidate for meat quality traits. In this study, variations on the exonic regions of the porcine MICU1 gene were investigated by sequencing cDNAs and tested for their association with meat quality traits. A total of 667 Berkshire heads (347 sows and 320 castrated boars) were used for this association test. Three SNPs were detected on the 3' untranslated region (UTR) of the porcine MICU1 gene. SNP1 (c.*136G>A) was associated with drip loss (p=0.017) and intramuscular fat content (p=0.039). In addition, SNP2 (c.*222G>A) and SNP3 (c.*485G>A) were associated with drip loss (p=0.018) and intramuscular fat content (p<0.001), respectively. In conclusion, it was verified that three variations on the 3' UTR of the porcine MICU1 gene were significantly associated with meat quality traits. It was also suggested that molecular biological analyses are needed to validate the function of variations on the 3 UTR of the porcine MICU1 gene.

Mitochondrial calcium uptake 1 (MICU1)은 2개의 canonical EF hands를 가지고 미토콘드리아 내막에 위치하여, 미토콘드리아의 칼슘 섭취에 중요한 기능을 하는 것으로 알려져 있다. 근육 세포의 미토콘드리아 칼슘 섭취는 사후에 급속냉각 또는 냉장 시, 근육 내 칼슘 방출로 인한 강직과 관련되어 있으므로 궁극적으로는 육질 형성에 관련이 있을 것으로 판단된다. 따라서 본 연구에서는 돼지 MICU1 유전자의 exon영역의 변이를 탐색하고, 발굴된 변이에 대해 육질 형질과의 연관성 분석을 실시하였다. 이를 위해 버크셔 667두(암퇘지 347두, 거세수퇘지 320두)가 이용되었으며, MICU1 유전자의 cDNA를 염기서열 해독하여 비교함으로써 exon 영역의 변이를 발굴하였다. 그 결과 MICU1의 3' 비해독 영역(untranslated region, UTR)에서 3개의 단일염기다형성(single-nucleotide polymorphism, SNP)를 발견했다. 그리고 이들 SNP에 대해 공시돈의 육질형질(근육 pH, 육즙 손실, 육색, 근내지방함량)과 연관성 분석을 실시했다. SNP1 (c.*136G>A)에서는 육즙 손실(p=0.017), 근내지방함량(p=0.039)과 연관되어 있었고, SNP2 (c.*222G>A)와 SNP3 (c.*485G>A)에서는 각각 육즙 손실(p=0.018)과 근내지방함량(p<0.001)과 연관되어 있는 것을 확인하였다. 따라서 본 결과를 바탕으로, 돼지에서 육질과 관련된 후보 유전자로 추정된 MICU1 유전자로부터 3' 비해독 영역의 변이가 유의적으로 육질 형질과 관련되어있다는 것이 확인되었다. 향후 MICU1 유전자의 3' 비해독 영역의 변이들에 기능적 역할을 정확히 파악하기 위한 분자생물학적 특성 연구가 필요할 것으로 판단된다.

Keywords

References

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