Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antimicrobial and Antioxidant Effects of Functional Healthy Drinks from Some Medicinal Herbs and Coffee Mixture

약용식물과 커피 혼합물로부터 기능성 건강음료의 항균 및 항산화 효과

  • Kim, In Hae (Department of Pharmaceutical Engineering, College of Medical & Life Science, Silla University) ;
  • Kim, JinKyun (The Gil Wondu Coffee) ;
  • Lee, Jae Hwa (Department of Pharmaceutical Engineering, College of Medical & Life Science, Silla University)
  • 김인혜 (신라대학교 의생명과학대학 제약공학과) ;
  • 김진균 (더 길 원두커피) ;
  • 이재화 (신라대학교 의생명과학대학 제약공학과)
  • Received : 2015.12.11
  • Accepted : 2016.10.13
  • Published : 2016.11.30
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Abstract

In this study, our investigated the antioxidant activities and compared other bioassay including anti-microbial, antifungal activities and total polyphenol contents of functional healthy drinks from some medicinal herbs and coffee mixture. The kind of medicinal herbs, chaga mushroom (Inonatus obliqurs), moringa (Moringa Oleifera), gravila (Anona muricata), mulberry (Morus alba), Dioscoreaceae (Dioscorea quinquelaba), Berberidaceae (Epimedii Herba), Asteraceae (Artemisia capillaries) and siberian ginseng (Acanthopanax senticous,). The functional healthy drinks, named C1, C2, C3, C4, C5, C6 and C7 were summered in Table 1. The in vitro antimicrobial activity was examined against Gram positive bacteria, Gram negative bacteria and a fungus. The functional healthy drinks were broad spectrum of anti-microbial activity without antifungal activity against Candida albicans KCTC7965. In particularly, the C7 showed strong activity against Methicillin Resistant Staphylococcus aureus CCARM3089, CCARM 3115 and CCARM3561. And, the C7 showed 88% of free radical scavenging effect on 0.5 mg/ml using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Functional healthy drink C7 was mulberry extracts from Morus alba, chaga mushroom from Inonatus obliqurs and moringa from Moringa olifera in additionally coffee extracts. Its results confirm that the potential use of mulberry extracts as a good source of antibacterial compounds or as a health promoting food and health drinks.

본 연구에서 기존의 생리활성이 알려진 차가 버섯(Inonatus obliquus), 모링가(Moringa oleifera), 그라비올라(Anona muricata), 단풍마(Dioscorea quinquelaba), 가시오가피(Acanhopanax senticosus), 음양곽(Epimedii herba), 인진쑥(Artemisia capillaris), 그리고 오디(Morus alba) 추출물과 원두커피를 혼합하여 기능성 음료를 제조하였으며, 각각의 음료를 C1에서 C7으로 명명하였다. 각 음료의 항균, 항곰팡이, 항산화 활성 및 총 폴리페놀 함량을 조사하였다. 항균 및 항곰팡이에 대한 활성은 Gram positive bacteria인 Staphylococcus aureus에 대하여 C4, C5, C6 및 C7이 활성을 나타내었고, 특히 C7인 경우 항생제 내성균주인 MRSA에 대하여 강한 활성을 나타내었다. Gram negative bacteria에 대한 결과는 E. coli D31에 대하여 1.0 mg/ml의 농도에서 C1에서 C7 모든 음료가 활성을 나타내었고, 반면에 곰팡이인 Candida albicans에 대하여는 활성을 나타내지 않았다. 총 폴리페놀 함량은 C6의 경우가 7.37 mg/g으로 가장 높은 결과를 나타내었고, C5의 경우는 3.78 mg/g으로 가장 낮은 함량을 나타내었다. DPPH로 항산화 능력을 측정한 결과, C1에서 C7 모두 강한 활성을 나타내었고, 특히 C7인 경우 0.5 mg/ml의 농도에서 88%라는 높은 free radical 소거능력을 나타내었다. 이러한 결과를 바탕으로 각 기능성 음료의 생리활성의 기초자료를 제공하고 다양한 기능성 건강음료뿐 만 아니라 고부가가치 기능성 제품을 창출 할 수 있는 근거를 마련한 것으로 생각된다.

Keywords

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