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무제치늪에 자생하는 식물의 뿌리에서 분리한 내생진균의 군집분석 및 다양성 분석

Community Analysis of Endophytic Fungal strains Isolated from the Roots of Plants Inhabiting Mujechi-neup

  • 천우재 (경북대학교 생명과학부) ;
  • 최혜림 (경북대학교 생명과학부) ;
  • 김현 (경북대학교 생명과학부) ;
  • 남윤종 (경북대학교 생명과학부) ;
  • 오유선 (경북대학교 생명과학부) ;
  • 정민지 (경북대학교 생명과학부) ;
  • 이난영 (경북대학교 생명과학부) ;
  • 하상철 (대구미래대학교 제과데코레이션학과) ;
  • 김종국 (경북대학교 생명과학부)
  • Cheon, Woo-Jae (Department of Life Sciences and Biotechnology, Institute for Microorganisms, Kyungpook National University) ;
  • Choi, Hye-Rim (Department of Life Sciences and Biotechnology, Institute for Microorganisms, Kyungpook National University) ;
  • Kim, Hyun (Department of Life Sciences and Biotechnology, Institute for Microorganisms, Kyungpook National University) ;
  • Nam, Yoon-Jong (Department of Life Sciences and Biotechnology, Institute for Microorganisms, Kyungpook National University) ;
  • Oh, Yoosun (Department of Life Sciences and Biotechnology, Institute for Microorganisms, Kyungpook National University) ;
  • Jeong, Minji (Department of Life Sciences and Biotechnology, Institute for Microorganisms, Kyungpook National University) ;
  • Lee, Nan-Yeong (Department of Life Sciences and Biotechnology, Institute for Microorganisms, Kyungpook National University) ;
  • Ha, Sang-Chul (Department of Hotel Culinary Arts, Daegu Future College) ;
  • Kim, Jong-Guk (Department of Life Sciences and Biotechnology, Institute for Microorganisms, Kyungpook National University)
  • 투고 : 2016.09.26
  • 심사 : 2016.12.27
  • 발행 : 2016.12.30

초록

이 논문은 무제치늪에서 채집한 기장대풀(Isachne globosa Kuntze), 솔방울고랭이(Scirpus karuisawensis Makino), 이삭귀개(Utricularia racemosa Wall.), 좀개수염(Eriocaulon decemflorum Maxim.)등 4종의 식물 뿌리에서 서식하는 내생곰팡이의 분포 및 다양성을 확인하기 위해 조사하였다. 총 226 균주가 분리되었고, 3문(Phyla), 7강(Class), 10목(Order), 22과(Family), 31(genera)속으로 분리되었다. 내생진균에서 가장 많이 분리된 속은 Acephala (19.9%), Tolypocladium (16.3%), Neopestalotiopsis (11.5%), Perenniporia (7.1%) 순으로 확인하였다. 다양성지수 분석에 있어서, 이삭귀개(Ur)가 다른 식물 종에 비해 종 풍부도 (Menhinick's index = 2.37), (Margalef's index = 4.46)와, 종 균등도 (Simpson's index diversity = 0.91), 그리고 종 다양성(Shannon's indx = 2.57)로 높게 나왔다. 이러한 분석 결과를 봤을 때, 이삭귀개(Ur)에서 내생진균이 가장 다양하게 서식할 수 있는 것으로 보여진다.

Wetlands exhibit intermediate characteristics of both terrestrial and aquatic ecosystems, and the biodiversity is rich in these unique biological habitats. The symbiotic relationships between plants and fungi that inhabit these wetlands play an important role in natural resource management, biodiversity, and conservation. Accordingly, the mujechi, having academic value for the study of the natural environment, was investigated in terms of genetic diversity of endophytic fungi, which inhabit the roots of wild plants. The internal transcribed spacer (ITS) region was amplified to identify fungal strains. In total, 226 strains were isolated and categorized into three phyla, seven classes, 10 orders, 22 families, and 31 genera. In plants by endophytic fungi were classified in Isachne globosa (Ig) to 19 genera, Scirpus karuisawensis (Sk) to 11 genera, Utricularia racemosa (Ur) to 19 genera, and one incertae sedis, Eriocaulon decemflorum (Ed) to 11 genera. The fungal taxa was identified the genera Acephala (19.9%), Tolypocladium (16.3%), Neopestalotiopsis (11.5%), and Perenniporia (7.1%). The fungal group isolated from Isachne globosa (Ig) grew the largest number of isolated fungal strains. After comprehensive evaluation, the endophytic fungal group from Utricularia racemosa (Ur) ranked highest in diversity analyses. From the roots of wild plant in mujechi-neup, it confirmed the distribution and diversity of endophytic fungi. This study provides the basic data to understand fungal community structure in peat wetlands.

키워드

참고문헌

  1. Allen, M. F., Swenson, W., Querejeta, J. I., Egerton-Warburton, L. M. and Treseder, K. K. 2003. Ecology of mycorrhizae: A conceptual framework for complex interactions among plants and fungi. Annu. Rev. Phytopathol. 41, 271-303. https://doi.org/10.1146/annurev.phyto.41.052002.095518
  2. Ayers, S., Zink, D. L., Mohn, K., Powell, J. S., Brown, C. M., Bills, G., Grund, A., Thompson, D. and Singh, S. B. 2010. Anthelmintic constituents of Clonostachys candelabrum. J. Antibiotics. 63, 119-122. https://doi.org/10.1038/ja.2009.131
  3. Bae, J. J., Choo, Y. S. and Song, S. D. 2003. The patterns of inorganic cations, nitrogen and phosphorus of plants in moojechi moor on Mt. Jeongjok. Soil Biol. Biochem. 31, 965-977.
  4. Brotman, Y., Cuadros-Inostroza, A., Takayuki, T., Fernie, A.R., Chet, I., Viterbo, A. and Willmitzer, L. 2013. Trichodermaplant root colonization: Escaping early plant defense responses and activation of the antioxidant machinery for saline stress tolerance. PloS Pathog. 9: e1003221. doi:10.1371/journal.ppat.1003221
  5. Chamorro, M., Aguado, A. and De los Santos, B. 2016. First report of root and crown rot caused by Pestalotiopsis clavispora (Neopestalotiopsis clavispora) on strawberry in Spain. J. APS. 100, 1495.
  6. Cheon, W. and Jeon, Y. 2016. First report of anthracnose caused by Colletotrichum spaethianum of Fragrant plantain lily in Korea. J. APS. 7, 1498.
  7. Clay, K. And Holah, J. 1999. Fungal endophyte symbiosis and plant diversity in successional fields. Science 285, 10.1126/science.285.5434.1742.
  8. Decock, C. 2006b. Studies in Perenniporia: Polyporus unitus, Boletus medulla-panis, the nomenclature of Perenniporia, Poria and Physisporus, and a note on europcan Perenniporia with a resupinate basidiome. Taxon 55, 759-778. https://doi.org/10.2307/25065650
  9. Gianinazzi-Pearson, Vivienne. 1996. Plant cell responses to arbuscular mycorrhizal fungi: Getting to the roots of the symbiosis. Plant Cell. 8, 1871-1883. https://doi.org/10.1105/tpc.8.10.1871
  10. Goettel, M. S. 1988. Pathogenesis of the hyphomycete Tolypocladium cylindrosporum in the mosquito Aedes aegypti. J. Invert. Pathol. 51, 259-274. https://doi.org/10.1016/0022-2011(88)90033-X
  11. Grünig, C. R. and Sieber, T. N. 2005. Molecular and phenotypic description of the widespread root symbiont Acephala applanata gen. Et sp. Nov., formerly known as dark-septate endophyte Type 1. Mycologia 97, 628-640 https://doi.org/10.1080/15572536.2006.11832794
  12. Hill, M. O. 1973. Diversity and evenness: A unifying notation and its consequences. Ecology 54, 427-432. https://doi.org/10.2307/1934352
  13. Hill, T. C., Walsh, K. A., Harris, J. A. and Moffett, B. F. 2003. Using ecological diversity measures with bacterial communities. FEMS Microbiol. Ecol. 43, 1-11. https://doi.org/10.1111/j.1574-6941.2003.tb01040.x
  14. Howell, C. R. 2002. Mechanisms employed by Trichoderma species in the biological control of plant diseases: The history and evolution of current concepts. Phytopathol. 87, D-2002-1028-01F.
  15. Jost, L. 2006. Entropy and diversity. Ecology 113, 363-75.
  16. Khan, S. A., Hamayun, M., Yoon, H. J., Kim, H. Y., Suh, S. J., Hwang, S. K., Kim, J. M. and Kim, J. G. 2008. Plant growth promotion and Penicillium citrinum. BMC Microbiol. 8, 231. https://doi.org/10.1186/1471-2180-8-231
  17. Khan, S. A., Hamayun, M., Kim, H. Y., Yoon, H. J., Seo, J. C., Choo, Y. S., Lee, I. J., Kim, S. D., Rhee, I. K. S. J. and Kim, J. G. 2009. A new strain of Arthrinium phaeospermum isolated from Carex kobomugi Ohwi is capable of gibberellin production. Biotechnol. Lett. 31, 283-287. https://doi.org/10.1007/s10529-008-9862-7
  18. Kil, Y. J., Eo, J. K. and Eom, A. H. 2009. Molecular identification and diversity of endophytic fungi isolated from Pinus densiflora in Boeun, Korea. Kor. J. Mycol. 37, 130-133. https://doi.org/10.4489/KJM.2009.37.2.130
  19. Kim, H., You, Y. H. Yoon, H., Seo, Y., Kim, Y. E., Choo, Y. S., Lee, I. J., Shin, J. H. and Kim, J. G. 2014. Culturable fungal endophytes isolated from the root of coastal plant inhabiting korean east coast. Mycobiology 42, 100-108. https://doi.org/10.5941/MYCO.2014.42.2.100
  20. Kim, H. G., Jeong, J. Y. and Koo, B. H. 2010. The identification and vegetation structure of several mountainous wetlands in Dan-yang and around area. J. Kor. Env. Res. Tech. 13, 1-13.
  21. Kim, J. W. and Kim, J. H. 2003. Vegetation of moojechi moor in Ulsan: Syntaxonomy and syndynamics. Kor. J. Ecol. 26, 281-287. https://doi.org/10.5141/JEFB.2003.26.5.281
  22. Lugo, A. E., Brown, S. and Brinson, M. M. 1988. Forested wetlands in freshwater and salt-water environments. Limnol. Oceanogr, Inc. 33, 894-909.
  23. Moreno, C. A., Castillo, F., Gonzalez, A., Bernal, D., Jaimes, Y., Chaparro, M., Gonzalez, C., Rodriguez, F., Restrepo, S. and Cotes, A. M. 2009. Biological and molecular characterization of the response of tomato plants treated with Trichoderma koningiopsis. Physol. Plant Pathol. 74, 111-120. https://doi.org/10.1016/j.pmpp.2009.10.001
  24. Park, J. K. and Chang, N. K. 1998. Past vegetation of moojaechi on Mt. Jungjok by pollen analysis. Kor. J. Ecol. 21, 427-433.
  25. Park, S. J., An, B. R., Jang, S. Y. and Park, S. J. 2011. Diversity of moojechineup's flora. Kor. J. PI. Taxon. 41, 376-382.
  26. Quilliam, R. S. and Jones, D. 2010. Fungal root endophytes of the carnivorous plant Drosera rotundifolia. Mycorrhiza 20, 341-348. https://doi.org/10.1007/s00572-009-0288-4
  27. Santana, K. F. A., Garcia, C. B., Matos, K. S. and Hanada, R. E. 2016. First report of anthracnose caused by Colletotrichum spaethianum on Allium fistulosum in Brazil. J. APS. 1, 224.
  28. Schreiner, R. P., Mihara, K. L., McDanie,l H. and Bethlenfalvay, G. J. 1997. Mycorrhizal fungi influence plant and soil functions and interactions. Plant Soil 188, 199-209. https://doi.org/10.1023/A:1004271525014
  29. Smolander, A. and Priha, O. 1999. Nitrogen transformations in soil under Pinus sylvestris, Picea abies and Betula pendula at two forest sites. Kor. J. Ecol. 26, 109-114.
  30. Son, H. J., Kim, Y. S., Kim, S. C., Kim, S. C., Lee, H. B. and Park, W. G. 2015. Plants species diversity and flora of wetlands in the forest of Gangwon province. Kor. J. Plant Res. 28, 419-440. https://doi.org/10.7732/kjpr.2015.28.4.419
  31. Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30, 2725-2729. https://doi.org/10.1093/molbev/mst197
  32. Van der H., Marcel, G. A., John, N. K., Margot, U., Peter, M., Ruth, S., Thomas, B., Andres, W. and Ian, R. S. 1998. Mycorrhizal fungal diversity determines plant biodiversity ecosystem variability and productivity. Nature 396, 69-72. https://doi.org/10.1038/23932
  33. Vazquez, M. M., Cesar, S., Azcon, R. and Barea, J. M. 2000. Interactions between arbuscular myorrhizal fungi and other microbial inoculants (Azospirillum, Pseudomonas, Trichoderma) and their effects on microbial population and enzyme activities in the rhizosphere of maize plants. Appl. Soil Ecol. 15, 261-272. https://doi.org/10.1016/S0929-1393(00)00075-5
  34. Vinale, F., Sivasithamparam, K., Ghisalberti, E. L., Marra, R., Woo, S. L. and Lorito, M. 2008. Trichoderma-plant-pathogen interactions. Soil Biol. Biochem. 40, 1-10. https://doi.org/10.1016/j.soilbio.2007.07.002
  35. Yamada, A., Ogura, T., Degawa, Y. and Ohmasa, M. 2001. Isolation of Tricholoma matsutake and T. Bakamatsutake cultures from field-collected ecotomycorrhizas. Mycoscience 42, 43-50. https://doi.org/10.1007/BF02463974
  36. You, Y. H., Yoon, H, Woo, J. R., Seo, Y, Shin, J. H., Choo, Y. S., Lee, I. J. and Kim, J. G. 2011. Plant growth promotion activitiy of endophytic fungi isolated from the roots of Calystegia soldanella. Kor. J. Microbiol. Biotechnol. 39, 324-329.
  37. You, Y. H., Yoon, H., Woo, J. R., Seo, Y., Kim, M., Lee, G. and Kim, J. G. 2012. Diversity of endophytic fungi from the roots of halophytes growing in go-chang salt marsh. J. Mycology 40, 86-92. https://doi.org/10.4489/KJM.2012.40.2.86
  38. Zabalgogeazcoa, I., Oleaga, A. and Perez-Sanchez, R. 2008. Pathogenicity of endophytic entomopathogenic fungi to Ornithodoros erraticus and Ornithodoros moubata (Acari: Argasidae). Veterinary Parasitol. 158, 336-343. https://doi.org/10.1016/j.vetpar.2008.09.019