Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Effects of Sample Preparation Methods for the Isolation of Foodborne Pathogens from Sprout Seeds

새싹채소 종자의 전처리 방법이 식중독 세균 검출에 미치는 영향

  • Kim, Won-Il (Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Sun Young (Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, In-Seon (Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Han, Sanghyun (Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Se-Ri (Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Yun, Bohyun (Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Ryu, Jae-Gee (Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Hyeon-Ju (Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration)
  • 김원일 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 김선영 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 김인선 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 한상현 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 김세리 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 윤보현 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 류재기 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 김현주 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀)
  • Received : 2016.08.26
  • Accepted : 2016.09.22
  • Published : 2016.12.30
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Abstract

Sample preparation methods were evaluated for effectiveness in detecting foodborne pathogens from sprout seeds. The methods included: Rinse.-Test portions were rinsed with 0.1% peptone water, and the pellet after centrifugation was inoculated into pre-enrichment media; and Sprouting.-Seed samples were sprouted before pre-enrichment and sprouted seeds were inoculated into pre-enrichment media. In rinse method, E. coli was isolated from 13 of 280 sample units. In sprouting method, E. coli was isolated from 12 of 135 sample units. E. coli O157:H7, Salmonella spp., and L. monocytogenes were not detected in any of the samples. In the trials for recovering Salmonella enterica from artificially contaminated alfalfa seeds, the soak, rinse, and sprouting methods were evaluated. The detection rates of S. enterica were statistically different according to the amount of the sample tested and selective medium type (P < 0.05).

본 연구는 전처리 방법이 새싹채소 재배에 사용되는 종자 중 식중독 세균의 검출율에 영향을 미치는지에 대해 검증하기 위해 시중 유통되고 있는 새싹채소 재배용 종자 19종을 수집하여 종자를 세척 방법과 발아 방법으로 전처리한 후 E. coli, E. coli O157:H7, Salmonella spp., L. monocytogenes의 검출율을 비교하였다. 또한 인위적으로 Salmonella enterica를 접종한 알팔파 종자에 대해서 무처리, 세척, 발아 방법으로 전처리한 후 S. enterica의 검출율을 검정하였다. 시중 유통되고 있는 종자를 수집하여 분석한 결과, 세척 방법과 발아 방법 등의 전처리 방법에 따라 E. coli 검출율과 양성시료에 차이가 있었음을 확인하였다. 인위적으로 S. enterica를 접종한 알팔파 종자에 대한 검출율 비교 검정에서도 분석시료량 대비 전처리방법별로 검출율이 차이가 나는 것으로 나타났고, 선택배지 종류에 따라서도 S. enterica의 검출율이 차이가 났다. 결론적으로 새싹채소 재배용 종자의 식중독 세균 분석에 있어서 종자의 전처리 방법, 시료당 분석시료량, 선택배지 종류 등이 검출율에 영향을 미치는 것으로 나타났다.

Keywords

References

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