Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant Compounds and Antioxidant Activities of Methanolic Extracts from Milling Fractions of Oat

귀리 도정 부위별 메탄올 추출물의 항산화 성분 및 항산화 활성

  • Ham, Hyeonmi (Department of Central Area, National Institute of Crop Science, Rural Development Administration) ;
  • Woo, Koan Sik (Department of Central Area, National Institute of Crop Science, Rural Development Administration) ;
  • Park, Ji-Young (Department of Central Area, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Byongwon (Department of Central Area, National Institute of Crop Science, Rural Development Administration) ;
  • Choi, Hye Sun (Department of Central Area, National Institute of Crop Science, Rural Development Administration) ;
  • Choi, Yong-Hwan (Department of Central Area, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Junsoo (Department of Food and Animal Sciences, Chungbuk National University) ;
  • Lee, Yu-Young (Department of Central Area, National Institute of Crop Science, Rural Development Administration)
  • 함현미 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 우관식 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 박지영 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 이병원 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 최혜선 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 최용환 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 이준수 (충북대학교 식품생명.축산과학부) ;
  • 이유영 (농촌진흥청 국립식량과학원 중부작물부)
  • Received : 2016.07.20
  • Accepted : 2016.10.07
  • Published : 2016.11.30
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Abstract

Oats (Avena sativa L.) are well known for having high contents of bioactive compounds and health benefits. The objective of this study was to evaluate the antioxidant compounds and antioxidant activities of methanolic extracts from milling fractions and whole grains of oat. The contents of total polyphenolics and flavonoids were analyzed by spectrophotometric methods. ABTS radical and DPPH radical scavenging activities and reducing power were used to compare relative antioxidant activities of milling fractions from oat. Total polyphenolics and flavonoids were much more abundant in oat bran extract than in extracts from whole grain and endosperm. In addition, high levels of ABTS radical (42.34 mg Trolox equivalent antioxidant capacity (TEAC)/100 g sample) and DPPH radical (24.18 mg TEAC/100 g sample) scavenging activities and reducing power ($A_{700}=0.76$) were detected in oat bran. The results of this study indicate that oat bran has significantly higher antioxidant activities and appears to have significant health benefits.

본 연구에서는 국내에서 개발된 귀리의 도정 부위별 메탄올 추출물의 항산화 성분 및 항산화 활성을 비교 분석하고자 하였다. 항산화 성분으로는 총 폴리페놀 및 플라보노이드 함량을 측정하였으며 항산화 활성은 ABTS 및 DPPH 라디칼 제거능, 환원력을 측정하였다. 실험 결과 귀리의 도정 부위별 메탄올 추출물 중 bran 추출물의 총 폴리페놀 및 플라보노이드 함량이 각각 41.53 mg gallic acid equivalents/100 g sample 및 12.07 mg catechin equivalents/100 g sample로 whole grain 및 endosperm 추출물보다 높은 함량이 함유되어 있는 것을 확인하였다. 또한, 이러한 항산화 성분이 다량 분포하는 bran 추출물의 ABTS 및 DPPH 라디칼 제거능, 환원력이 각각 42.34 mg Trolox equivalent antioxidant capacity(TEAC)/100 g sample, 24.18 mg TEAC/100 g sample, $A_{700}=0.76$으로 나타나 whole grain 및 endosperm 추출물보다 우수한 항산화 활성을 가지는 것으로 나타났다. 본 연구 결과는 귀리의 도정 부위별 항산화 성분 및 항산화 활성 연구에 있어 기초 자료로써 활용될 수 있을 것으로 생각하며, 우수한 항산화 성분 및 활성을 가지는 귀리의 bran층은 기능성 식품의 제조에 있어 좋은 소재로 활용될 수 있을 것으로 생각한다.

Keywords

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