Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Effects of proanthocyanidin, a crosslinking agent, on physical and biological properties of collagen hydrogel scaffold

  • Choi, Yoorina (Department of Conservative Dentistry, Wonkwang University Dental Hospital) ;
  • Kim, Hee-Jin (Department of Conservative Dentistry, School of Dentistry, Chonbuk National University) ;
  • Min, Kyung-San (Department of Conservative Dentistry, School of Dentistry, Chonbuk National University)
  • Received : 2016.07.20
  • Accepted : 2016.08.27
  • Published : 2016.11.30
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Abstract

Objectives: The purpose of the present study was to evaluate the effects of proanthocyanidin (PAC), a crosslinking agent, on the physical properties of a collagen hydrogel and the behavior of human periodontal ligament cells (hPDLCs) cultured in the scaffold. Materials and Methods: Viability of hPDLCs treated with PAC was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The physical properties of PAC treated collagen hydrogel scaffold were evaluated by the measurement of setting time, surface roughness, and differential scanning calorimetry (DSC). The behavior of the hPDLCs in the collagen scaffold was evaluated by cell morphology observation and cell numbers counting. Results: The setting time of the collagen scaffold was shortened in the presence of PAC (p < 0.05). The surface roughness of the PAC-treated collagen was higher compared to the untreated control group (p < 0.05). The thermogram of the crosslinked collagen exhibited a higher endothermic peak compared to the uncrosslinked one. Cells in the PAC-treated collagen were observed to attach in closer proximity to one another with more cytoplasmic extensions compared to cells in the untreated control group. The number of cells cultured in the PAC-treated collagen scaffolds was significantly increased compared to the untreated control (p < 0.05). Conclusions: Our results showed that PAC enhanced the physical properties of the collagen scaffold. Furthermore, the proliferation of hPDLCs cultured in the collagen scaffold crosslinked with PAC was facilitated. Conclusively, the application of PAC to the collagen scaffold may be beneficial for engineering-based periodontal ligament regeneration in delayed replantation.

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References

  1. Krasner P, Rankow HJ. New philosophy for the treatment of avulsed teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1995;79:616-623. https://doi.org/10.1016/S1079-2104(05)80105-2
  2. Hermann NV, Lauridsen E, Ahrensburg SS, Gerds TA, Andreasen JO. Periodontal healing complications following extrusive and lateral luxation in the permanent dentition: a longitudinal cohort study. Dent Traumatol 2012;28:394-402. https://doi.org/10.1111/edt.12000
  3. Andreasen JO, Borum MK, Jacobsen HL, Andreasen FM. Replantation of 400 avulsed permanent incisors. 4. Factors related to periodontal ligament healing. Endod Dent Traumatol 1995;11:76-89. https://doi.org/10.1111/j.1600-9657.1995.tb00464.x
  4. Mattioli-Belmonte M, Teti G, Salvatore V, Focaroli S, Orciani M, Dicarlo M, Fini M, Orsini G, Di Primio R, Falconi M. Stem cell origin differently affects bone tissue engineering strategies. Front Physiol 2015;6:266.
  5. Wang Y, Cheung GS, Xu X, Zhao S, Zhang C. The effect of cultured autologous periodontal ligament cells on the healing of delayed autotransplanted dog's teeth. J Endod 2010;36:264-267. https://doi.org/10.1016/j.joen.2009.09.014
  6. Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, Young M, Robey PG, Wang CY, Shi S. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet 2004;364:149-155. https://doi.org/10.1016/S0140-6736(04)16627-0
  7. Lekic P, Rojas J, Birek C, Tenenbaum H, McCulloch CA. Phenotypic comparison of periodontal ligament cells in vivo and in vitro. J Periodont Res 2001;36:71-79. https://doi.org/10.1034/j.1600-0765.2001.360202.x
  8. Yang ZH, Zhang XJ, Dang NN, Ma ZF, Xu L, Wu JJ, Sun YJ, Duan YZ, Lin Z, Jin Y. Apical tooth germ cellconditioned medium enhances the differentiation of periodontal ligament stem cells into cementum/ periodontal ligament-like tissues. J Periodontal Res 2009;44:199-210. https://doi.org/10.1111/j.1600-0765.2008.01106.x
  9. Huang GT, Gronthos S, Shi S. Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine. J Dent Res 2009;88:792-806. https://doi.org/10.1177/0022034509340867
  10. Caton J, Bostanci N, Remboutsika E, De Bari C, Mitsiadis TA. Future dentistry: cell therapy meets tooth and periodontal repair and regeneration. J Cell Mol Med 2011;15:1054-1065. https://doi.org/10.1111/j.1582-4934.2010.01251.x
  11. Han J, Menicanin D, Gronthos S, Bartold PM. Stem cells, tissue engineering and periodontal regeneration. Aust Dent J 2014;59(Supplement 1):117-130. https://doi.org/10.1111/adj.12100
  12. Zhou Y, Li Y, Mao L, Peng H. Periodontal healing by periodontal ligament cell sheets in a teeth replantation model. Arch Oral Biol 2012;57:169-176. https://doi.org/10.1016/j.archoralbio.2011.08.008
  13. Ivanovski S, Vaquette C, Gronthos S, Hutmacher DW, Bartold PM. Multiphasic scaffolds for periodontal tissue engineering. J Dent Res 2014;93:1212-1221. https://doi.org/10.1177/0022034514544301
  14. Davidenko N, Schuster CF, Bax DV, Raynal N, Farndale RW, Best SM, Cameron RE. Control of crosslinking for tailoring collagen-based scaffolds stability and mechanics. Acta Biomater 2015;25:131-142 https://doi.org/10.1016/j.actbio.2015.07.034
  15. Hutmacher DW, Cool S. Concepts of scaffold-based tissue engineering: the rationale to use solid free-form fabrication techniques. J Cell Mol Med 2007;11:654-669. https://doi.org/10.1111/j.1582-4934.2007.00078.x
  16. Wang Y, Van Manh N, Wang H, Zhong X, Zhang X, Li C. Synergistic intrafibrillar/extrafibrillar mineralization of collagen scaffolds based on a biomimetic strategy to promote the regeneration of bone defects. Int J Nanomedicine 2016;11:2053-2067.
  17. Zhang J, Deng A, Yang Y, Gao L, Xu N, Liu X, Hu L, Chen J, Yang S. HPLC detection of loss rate and cell migration of HUVECs in a proanthocyanidin cross-linked recombinant human collagen-peptide (RHC)-chitosan scaffold. Mater Sci Eng C Mater Biol Appl 2015;56:555-563. https://doi.org/10.1016/j.msec.2015.07.019
  18. Fortunati D, Chau DY, Wang Z, Collighan RJ, Griffin M. Cross-linking of collagen I by tissue transglutaminase provides a promising biomaterial for promoting bone healing. Amino Acids 2014;46:1751-1761. https://doi.org/10.1007/s00726-014-1732-0
  19. Castellan CS, Bedran-Russo AK, Antunes A, Pereira PN. Effect of dentin biomodification using naturally derived collagen cross-linkers: one-year bond strength study. Int J Dent 2013;2013:918010.
  20. Ma L, Gao C, Mao Z, Zhou J, Shen J. Enhanced biological stability of collagen porous scaffolds by using amino acids as novel cross-linking bridges. Biomaterials 2004;25:2997-3004. https://doi.org/10.1016/j.biomaterials.2003.09.092
  21. Bedran-Russo AK, Pauli GF, Chen SN, McAlpine J, Castellan CS, Phansalkar RS, Aguiar TR, Vidal CM, Napotilano JG, Nam JW, Leme AA. Dentin biomodification: strategies, renewable resources and clinical applications. Dent Mater 2014;30:62-76. https://doi.org/10.1016/j.dental.2013.10.012
  22. Bagchi D, Bagchi M, Stohs Sj, Ray SD, Sen CK, Preuss HG. Cellular protection with proanthocyanidins derived from grape seeds. Ann N Y Acad Sci 2002;957:260-270. https://doi.org/10.1111/j.1749-6632.2002.tb02922.x
  23. Ye X, Krohn RL, Liu W, Joshi SS, Kuszynski CA, McGinn TR, Bagchi M, Preuss HG, Stohs SJ, Bagchi D. The cytotoxic effects of a novel IH636 grape seed proanthocyanidin extract on cultured human cancer cells. Mol Cell Biochem 1999;196:99-108. https://doi.org/10.1023/A:1006926414683
  24. Kato A, Miyaji H, Ishizuka R, Tokunaga K, Inoue K, Kosen Y, Yokoyama H, Sugaya T, Tanaka S, Sakagami R, Kawanami M. Combination of root surface modification with BMP-2 and collagen hydrogel scaffold implantation for periodontal healing in beagle dogs. Open Dent J 2015;9:52-59. https://doi.org/10.2174/1874210601509010052
  25. Zhu W, Zhang Q, Zhang Y, Cen L, Wang J. PDL regeneration via cell homing in delayed replantation of avulsed teeth. J Transl Med 2015;13:357. https://doi.org/10.1186/s12967-015-0719-2
  26. Han B, Jaurequi J, Tang BW, NimniME. Proanthocyanidin: a natural crosslinking reagent for stabilizing collagen matrices. J Biomed Mater Res A 2003;65:118-124.
  27. Deters A, Dauer A, Schnetz E, Fartasch M, Hensel A. High molecular compounds (polysaccharides and proanthocyanidins) from Hamamelis virginiana bark: influence on human skin keratinocyte proliferation and differentiation and influence on irritated skin. Phytochemistry 2001;58:949-958. https://doi.org/10.1016/S0031-9422(01)00361-2
  28. Neuwirt H, Arias MC, Puhr M, Hobisch A, Culig Z. Oligomeric proanthocyanidin complexes (OPC) exert anti-proliferative and pro-apoptotic effects on prostate cancer cells. Prostate 2008;68:1647-1654. https://doi.org/10.1002/pros.20829
  29. Xie ZY, Wu BH, Yang ZG, Chen XF, Chen QS. Differentiation of human promyelocytic leukemia HL-60 cells induced by proanthocyanidin and its mechanism. Zhongguo Shi Yan Xue Ye Xue Za Zhi 2013;21:920-925.
  30. Madaghiele M, Calo E, Salvatore L, Bonfrate V, Pedone D, Frigione M, Sannino A. Assessment of collagen crosslinking and denaturation for the design of regenerative scaffolds. J Biomed Mater Res A 2016;104:186-194. https://doi.org/10.1002/jbm.a.35554
  31. Faia-Torres AB, Guimond-Lischer S, Rottmar M, Charnley M, Goren T, Maniura-Weber K, Spencer ND, Reis RL, Textor M, Neves NM. Differential regulation of osteogenic differentiation of stem cells on surface roughness gradients. Biomaterials 2014;35:9023-9032. https://doi.org/10.1016/j.biomaterials.2014.07.015
  32. Kwon YS, Lim ES, Kim HM, Hwang YC, Lee KW, Min KS. Genipin, a cross-linking agent, promotes odontogenic differentiation of human dental pulp cells. J Endod 2015;41:501-507. https://doi.org/10.1016/j.joen.2014.12.002
  33. Lim ES, Lim MJ, Min KS, Kwon YS, Hwang YC, Yu MK, Hong CU, Lee KW. Effects of epicatechin, a crosslinking agent, on human dental pulp cells cultured in collagen scaffolds. J Appl Oral Sci 2016;24:76-84. https://doi.org/10.1590/1678-775720150383

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