Journal of the East Asian Society of Dietary Life (동아시아식생활학회지)

The East Asian Society of Dietary Life (동아시아식생활학회)
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Effect of Allium hookeri Root on Plasma Blood Glucose and Fat Profile Levels in Streptozotocin-Induced Diabetic Rats

당뇨 유발 흰쥐에서 삼채뿌리 첨가 식이가 혈당과 지질 수준에 미치는 영향

  • Kim, Myung-Wha (Dept. of Food and Nutrition, Duksung Women's University)
  • 김명화 (덕성여자대학교 식품영양학과)
  • Received : 2016.09.08
  • Accepted : 2016.10.27
  • Published : 2016.12.30
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Abstract

This study was designed to examine the effects of Allium hookeri (AH) on plasma blood glucose and fat profile levels in diabetic rats. Diabetes mellitus was induced in male Sprague-Dawley rats through injection of streptozotocin (STZ) dissolved in citrate buffer into tail veins at a dose of 45 mg/kg of body weight. Sprague-Dawley rats were then fed for 4weeks, with the experimental groups receiving a modified diet containing 5% or 10% powder derived from AH roots. The experimental groups were divided into four groups, consisting of a control group, STZ-control group, and diabetic fed with AH 5% & 10% treated groups. Rats' body weights, blood glucose, total cholesterol, HDL-cholesterol, triglyceride (TG), and free fatty acid (FFA) values in plasma were measured along with hematocrit (Hct) values and aminotransferase activities. Body weight losses were observed in the STZ-control group, whereas the STZ-AH group of diabetic rats gained weight. There was a significantly decrease in brain weight of the STZ-AH group but no significant differences in kidney and liver weights of the STZ-AH 5% & STZ-AH 10% groups compared to the STZ-control group. Blood glucose was significantly reduced in the STZ-AH 5% & STZ-AH 10% diabetic groups. There were no significant differences in total cholesterol and TG levels among the diabetic groups. HDL-cholesterol significantly increased while FFA significantly decreased in the STZ-AH 5% & STZ-AH 10% diabetic groups. The Hct level of the STZ-AH group was lower than that of the STZ-control group. Aspartate aminotransferase activity was significantly reduced in the STZ-AH 5% & STZ-AH 10% diabetic groups. These results indicate that supplementation with Allium hookeri root may have beneficial effects on diabetic complications as a potential therapeutic candidate.

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References

  1. Alberti KG, Zimmet PZ (1998) Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med 15(7): 539-553. https://doi.org/10.1002/(SICI)1096-9136(199807)15:7<539::AID-DIA668>3.0.CO;2-S
  2. Allain CC, Poon LS, Chan CS, Richmond W, Fu PC (1974) Enzymatic determination of total serum cholesterol. Clin Chem 20(4): 470-475.
  3. Ayam VS (2011) Allium hookeri, Thw. Enum. A lesser known terrestrial perennial herb used as food and its ethnobotanical relevance in Manipur. African J Food, Agriculture, Nutrition and Development 11(6): 5389-5412.
  4. Bae GC, Bae DY (2012) The anti-inflammatory effects of ethanol extract of Allium hookeri cultivated in South Korea. Korean J Herbology 27(6): 55-61.
  5. Bauer JD (1982) Clinical Laboratory Methods. 9th ed. Mosby. St. Louis. pp 188-189.
  6. Borborah K, Dutta B, Borthakur SK (2014) Traditional uses of Allium L. species from north east india with special reference to their pharmacological activities. American J Phytomedicine and Clinical Therapeutics 2(8): 1037-1051.
  7. Choi JW, Sohn KH, Kim SH (1991) The effects of nicotinamide on the serum lipid composition in streptozotocin induced diabetic rats. J Korean Soc Food Nutr 20(4): 306-311.
  8. Dashtban M, Sarir H, Omidi A (2016) The effect of Prosopis farcta beans extract on blood biochemical parameters in streptozotocin-induced diabetic male rats. Adv Biomed Res 5(116): 1-4. https://doi.org/10.4103/2277-9175.174955
  9. Ebaid H, Al-Tamimi J, Metwalli A, Allam A, Zohir K, Ajarem J, Rady A, Alhazza IM, Ibrahim KE (2015) Effect of STZ-induced diabetes on spleen of rats: Improvement by camel whey proteins. Pakistan J Zool 47(4): 1109-1116.
  10. Ebbert JO, Jensen MD (2013) Fat depots, free fatty acids, and dyslipidemia. Nutrients 5(2): 498-508. https://doi.org/10.3390/nu5020498
  11. Finely PR, Schifman RB, Williams RJ, Luchti DA (1978) Cholesterol in high-density lipoprotein: Use of $Mg^{2+}$/dextran sulfate in its measurement. Clin Chem 24(6): 931-933.
  12. Giegel JL, Ham AB, Clema W (1975) Manual and semi-automated procedures for measurements of triglycerides in serum. Clin Chem 21(11): 1575-1581.
  13. Gold G, Manning M, Heldt A, Nowlain R, Pettit JG, Grodsky GM (1981) Diabetic induced with multiple subdiabetogenic doses of streptozotocin. Diabetes 30(8): 634-638 https://doi.org/10.2337/diab.30.8.634
  14. Haraa T, Kashiharaa D, Ichimurac A, Kimuraa I, Tsujimotoa G, Hirasawaa A (2014) Role of free fatty acid receptors in the regulation of energy metabolism. Biochimica et Biophysica Acta 1841(9): 1292-1300. https://doi.org/10.1016/j.bbalip.2014.06.002
  15. He W, Goodkind D, Kowal P (2016) An aging world: 2015, international population reports, U.S. Census Bureau pp 161-165.
  16. Hwang JS, Lee BH, An X, Jeong HR, Kim YE, Lee I, Lee HJ, Kim DO (2015) Total phenolics, total flavonoids, and antioxidant capacity in the leaves, bulbs, and roots of Allium hookeri. Korean J Food Sci Technol 47(2): 261-266. https://doi.org/10.9721/KJFST.2015.47.2.261
  17. Jeong JH, Lee SH, Hue JJ, Lee KN, Nam SY, Yun YW, Jeong SW, LEE YH, Lee BJ (2008) Effect of bitter melon (Momordica charantia) on anti-diabetic activity in C57BL/6J db/db mice. Korean J Vet Res 48(3): 327-336.
  18. Jun HI, Park SY, Jeong DY, Song GS, Kim YS (2014) Quality properties of yogurt added with hot water concentrates from Allium hookeri root. J Korean Soc Food Sci Nutr 43(9): 1415-1422. https://doi.org/10.3746/jkfn.2014.43.9.1415
  19. Kanai I, Kanai M (1983) Compendium of the Clinical Inspection. 29th ed. Komoonsa, Seoul. p 467.
  20. Kim JS, Heo JS, Choi JW, Kim GD, Sohn KH (2015) Obese mouse via regulation of hepatic lipogenesis and glucose metabolism. J Life Science 25(10): 1081-1090. https://doi.org/10.5352/JLS.2015.25.10.1081
  21. Kim JW, Cho HR, Moon SB, Kim KY, Ku SK (2012) Synergic effects of bitter melon and ${\beta}$-glucan composition on STZ induced rat diabetes and its complications. J Microbiol Biotechnol 22(1): 147-155. https://doi.org/10.4014/jmb.1106.06037
  22. Kim KH, Kim HJ, Byun MW, Yook HS (2012) Antioxidant and antimicrobial activities of ethanol extract from six vegetables containing different sulfur compounds. J Korean Soc Food Sci Nutr 41(5): 577-583. https://doi.org/10.3746/jkfn.2012.41.5.577
  23. Kwon HS, Kim WH, Lee DY, Park SY (2014) Current status and need for a Korean diabetes prevention study. Public Health Weekly Report, KCDC 8(32): 746-753.
  24. Lee EB, Kim JH, Yang JH, Kim YS, Jun HI, Ki BH, Lee SH, Kim YS, Han SC, Kim DK (2015) Antioxidant and longevity properties of the root of Allium hookeri in caenorhabditis elegans. Kor J Pharmacogn 46(3): 234-242.
  25. Lee SH, Kim NS, Choi BK, Jang HH, Kim JB, Lee YM, Kim DK, Lee CH, Kim YS, Yang JH, Kim YS, Kim HJ, Lee SH (2015) Effects of Allium hookeri on lipid metabolism in type II diabetic mice. Kor J Pharmacogn 46(2): 148-153.
  26. Mukai M, Ozasa K, Hayashi K Kawai K (2002) Various SGOT/S-GPT ratios in nonviral liver disorders and related physicalconditions and life-style. Dig Dis Sci 47(3): 549-555. https://doi.org/10.1023/A:1017959801493
  27. Nyenwe EA, Kitabchi AE (2016) The evolution of diabetic ketoacidosis: An update of its etiology, pathogenesis and management. Metabolism 65(4): 507-521. https://doi.org/10.1016/j.metabol.2015.12.007
  28. Ojiako OA, Chikezie PC, Ogbujic AC (2016) Blood glucose level and lipid profile of alloxan-induced hyperglycemic rats treated with single and combinatorial herbal formulations. J Tradit Complement Med. 6(2): 184-192. https://doi.org/10.1016/j.jtcme.2014.12.005
  29. Park JY, Yoon KY (2014) Comparison of the nutrient composition and quality of the root of Allium hookeri grown in Korea and Myanmar. Korean J Food Sci Technol 46(5): 544-548. https://doi.org/10.9721/KJFST.2014.46.5.544
  30. Raabo E, Terkildsen TC (1960) On the enzymatic determination of blood glucose. Scandinav J Clin Lab Investigation 12: 402-407. https://doi.org/10.3109/00365516009065404
  31. Reeves PG (1997) Components of the AIN-93 diets as improvements in the AIN-76A diet. J Nutr 127(5): 838-841. https://doi.org/10.1093/jn/127.5.838S
  32. Reitman S, Frankel S (1957) A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28(1): 56-63. https://doi.org/10.1093/ajcp/28.1.56
  33. Rhyu DY, Park SH (2013) Characterization of alkyl thiosulfinate in Allium hookeri root using HPLC-ESI-MS. J Korean Soc Appl Biol Chem 56(4): 457-459. https://doi.org/10.1007/s13765-013-3069-x
  34. Roh SS, Kwon OJ, Yang JH, Kim YS, Lee SH, Jin JS, Jeon YD, Yokozawa T, Kim HJ (2016) Allium hookeri root protects oxidative stress-induced inflammatory responses and ${\beta}$-cell damage in pancreas of streptozotocin-induced diabetic rats. BMC Complement Altern Med 16(1): 63-72. https://doi.org/10.1186/s12906-016-1032-1
  35. Song EY, Pyun CW, Hong GE, Lim KW, Lee CH (2014) Effect of addition of Allium hookeri on the quality of fermented sausage with meat from sulfur fed pigs during ripening. Korean J Food Sci Anim Resour 34(3): 263-272. https://doi.org/10.5851/kosfa.2014.34.3.263
  36. Tahmasebpour N, Dehghan G, Feizi1 MAH, Esmaeili HA (2013) Variation in body weight and some hematological parameters in streptozotocin-induced diabetic rats, treated with teucrium orientale. Archives 3: 32-36.
  37. Taskinen MR, Boren J (2015) New insights into the pathophysiology of dyslipidemia in type 2 diabetes. Atherosclerosis 239(2): 483-495. https://doi.org/10.1016/j.atherosclerosis.2015.01.039
  38. Taylor R (2012) Insulin resistance and type 2 diabetes. Diabetes 61(4): 778-779. https://doi.org/10.2337/db12-0073
  39. Tuomilehto J, Lindstrom J, Eriksson JG, Valle TT, Hamalainen H, Ilanne-Parikka P, Keinanen-Kiukaanniemi S, Laakso M, Alouheranta A, Merja Rastas M, Salminen V, Aunola S, Cepaitis Z, Moltchanov V, Hakumäki M, Mannelin M, Martikkala V, Sundvall J, Uusitupa M (2001) Prevention of type 2 diabetes mellitus by changes in lifestyle among subjects with impaired glucose tolerance. N Engl J Med 344(18): 1343-1350. https://doi.org/10.1056/NEJM200105033441801
  40. Won JY, Yoo YC Kang EJ, Yang H, Kim GH, Seong BJ, Kim SI Han SH, Lee SS, Lee KS (2013) Chemical components, DPPH radical scavenging activity and inhibitory effects on nitric oxide production in Allium hookeri cultivated under open field and greenhouse conditions. J Korean Soc Food Sci Nutr 42(9): 1351-1356. https://doi.org/10.3746/jkfn.2013.42.9.1351
  41. World Health Organization (2016) Global report on diabetes.
  42. Wu L, Parhofer KG (2014) Diabetic dyslipidemia. Metabolism 63(12): 1469-1479. https://doi.org/10.1016/j.metabol.2014.08.010
  43. Yang HS, Choi YJ, Jin HY, Lee SC, Huh CK (2016) Effects of Allium hookeri root water extracts on inhibition of adipogenesis and GLUT-4 expression in 3T3-L1 adipocytes. Food Sci Biotechnol 25(2): 615-621. https://doi.org/10.1007/s10068-016-0086-7
  44. Zafar M, Naqvi SNH (2010) Effects of STZ-induced diabetes on the relative weights of kidney, liver and pancreas in albino rats: a comparative study. Int J Morphol 28(1): 135-142.

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