Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Simultaneous Determination of 11 Marker Compounds in Gumiganghwal-tang by HPLC-DAD and LC-MS

  • Weon, Jin Bae (Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University) ;
  • Jung, Youn Sik (Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University) ;
  • Ryu, Gahee (Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University) ;
  • Yang, Woo Seung (Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University) ;
  • Ma, Choong Je (Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University)
  • Received : 2016.04.06
  • Accepted : 2016.06.04
  • Published : 2016.12.31
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Abstract

Gumiganghwal-tang has been used for the treatment of common cold for a long-time. We developed an accurate and sensitive high performance liquid chromatography-diode array detection (HPLC-DAD) and electrospray ionization mass spectrometry method for the simultaneous determination of ferulic acid, baicalin, bergapten, methyl eugenol, glycyrrhizin, oxypeucedanin, wogonin, nodakenin, atractylenolide III, imperatorin, and atractylenolide I in Gumiganghwal-tang samples. The analytes were separated on a Shiseido C18 column ($5{\mu}m$, $4.6mm\;I.D.{\times}250mm$) with gradient elution with acetonitrile and 0.1% trifluoroacetic acid. Eleven compounds were quantitatively determined by HPLC-DAD and identified by LC-MS data. We also validated this method. The calibration curves of all the compounds showed good linear regression. The limits of detection and the limits of quantification ranged from 0.04 to 0.63 and from 0.12 to $1.92{\mu}g/mL$, respectively. The relative standard deviation values of intra- and inter-days of this method represented less than 2.9%. The recoveries were found to be in the range of 90.06 - 107.66%. The developed method has been successfully applied to the analysis of Gumiganghwaltang samples. The established HPLC method could be used to quality control of Gumiganghwal-tang.

Keywords

References

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