Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Genome Sequence of Bacillus cereus FORC_021, a Food-Borne Pathogen Isolated from a Knife at a Sashimi Restaurant

  • Chung, Han Young (Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University) ;
  • Lee, Kyu-Ho (Department of Life Science, Sogang University) ;
  • Ryu, Sangryeol (Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University) ;
  • Yoon, Hyunjin (Department of Applied Chemistry and Biological Engineering, Ajou University) ;
  • Lee, Ju-Hoon (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Kim, Hyeun Bum (Department of Animal Resources Science, Dankook University) ;
  • Kim, Heebal (Department of Animal Science and Biotechnology, Seoul National University) ;
  • Jeong, Hee Gon (Department of Food Science and Technology, Chungnam National University) ;
  • Choi, Sang Ho (Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University) ;
  • Kim, Bong-Soo (Department of Life Science, Hallym University)
  • Received : 2016.06.29
  • Accepted : 2016.08.15
  • Published : 2016.12.28
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Abstract

Bacillus cereus causes food-borne illness through contaminated foods; therefore, its pathogenicity and genome sequences have been analyzed in several studies. We sequenced and analyzed B. cereus strain FORC_021 isolated from a sashimi restaurant. The genome sequence consists of 5,373,294 bp with 35.36% GC contents, 5,350 predicted CDSs, 42 rRNA genes, and 107 tRNA genes. Based on in silico DNA-DNA hybridization values, B. cereus ATCC $14579^T$ was closest to FORC_021 among the complete genome-sequenced strains. Three major enterotoxins were detected in FORC_021. Comparative genomic analysis of FORC_021 with ATCC $14579^T$ revealed that FORC_021 harbored an additional genomic region encoding virulence factors, such as putative ADP-ribosylating toxin, spore germination protein, internalin, and sortase. Furthermore, in vitro cytotoxicity testing showed that FORC_021 exhibited a high level of cytotoxicity toward INT-407 human epithelial cells. This genomic information of FORC_021 will help us to understand its pathogenesis and assist in managing food contamination.

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References

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