The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Evaluation on Anti-oxidant Activity and Anti-inflammatory Effects for the New Formulation of Gamisoyosan

가미소요산의 새로운 제형에 대한 항산화 활성 및 항염증 효능평가

  • Choi, Hye-Min (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM)) ;
  • Kim, Se-Jin (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM)) ;
  • Kim, In-Su (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM)) ;
  • Lee, Ji-Beom (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM)) ;
  • Kim, Jong-Beom (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM)) ;
  • Moon, Sung-Ok (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM)) ;
  • Lee, Hwa-Dong (Korean Medicine Preparation Team, National Development Institute of Korean Medicine (NIKOM))
  • 최혜민 (한약진흥재단, 한약제제팀) ;
  • 김세진 (한약진흥재단, 한약제제팀) ;
  • 김인수 (한약진흥재단, 한약제제팀) ;
  • 이지범 (한약진흥재단, 한약제제팀) ;
  • 김종범 (한약진흥재단, 한약제제팀) ;
  • 문성옥 (한약진흥재단, 한약제제팀) ;
  • 이화동 (한약진흥재단, 한약제제팀)
  • Received : 2016.10.11
  • Accepted : 2016.11.16
  • Published : 2016.11.30
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Abstract

Objectives : Gamisoyosan (GMS) is a useful prescription for treating insomnia, dysmenorrhea and infertility induced by a stress. Also, GMS has been used traditionally to improve systemic circulation and biological energy production. The purpose of this study was to assess the anti-oxidant activity and anti-inflammatory effects of Gamisoyosan Formulation (Soft extract, GMS-SE). Methods : The biological activities such as anti-oxidant and anti-inflammatory effects were measured through cell line-based in vitro assay. We investigated the anti-oxidant properties of GMS-SE on the 1,1-diphenyl-2-picryhydrazyl (DPPH) radical, contents of total flavonoid and polyphenol. GMS-SE compared to butyl hydroxy anizole (BHA). Furthermore, based on this result the anti-inflammatory effects of GMS-SE have verified by mechanism from LPS- treated Raw264.7 macrophages. Results : The anti-oxidant activities of GMS-SE increased markedly, in a dose-dependent manner. The GMS-SE showed significant scavenging activity (GMS-SE $500{\mu}g/m{\ell}$ : $32.77{\pm}1.65%$, GMS-SE $1000{\mu}g/m{\ell}$ : $45.06{\pm}1.04%$ and BHA $100{\mu}g/m{\ell}$ : $39.25{\pm}2.41%$ for DPPH assay). and, The total phenolic compound and flavonoids contents of GMS-SE were $73.93{\pm}6.87{\mu}g/mg$ and $698.75{\pm}6.78{\mu}g/mg$. GMS-SE which is LPS has diminished in the LPS-induced release of inflammatory mediators (NO, iNOS, COX2 and PGE2) and pro-inflammatory cytokines (TNF-${\alpha}$, IL-6 and IL-$1{\beta}$) from the RAW264.7 macrophages. Moreover, GMS-SE inhibited the activation of phosphorylation of p38 and ERK MAPKs by induced LPS. Conclusion : The present results indicate that GMS-SE has an anti-oxidant and anti-inflammatory properties, therefore may be beneficial in diseases which related to oxidative stress-mediated inflammatory disorders.

Keywords

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