Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Polymorphisms in Heat Shock Proteins A1B and A1L (HOM) as Risk Factors for Oesophageal Carcinoma in Northeast India

  • Saikia, Snigdha (Department of Bioengineering and Technology, Laboratory of Molecular Virology and Oncology, Gauhati University Institute of Science and Technology, Gauhati University) ;
  • Barooah, Prajjalendra (Department of Bioengineering and Technology, Laboratory of Molecular Virology and Oncology, Gauhati University Institute of Science and Technology, Gauhati University) ;
  • Bhattacharyya, Mallika (Department of Gastroenterology, Gauhati Medical College Hospital) ;
  • Deka, Manab (Department of Bioengineering and Technology, Laboratory of Molecular Virology and Oncology, Gauhati University Institute of Science and Technology, Gauhati University) ;
  • Goswami, Bhabadev (Department of Gastroenterology, Gauhati Medical College Hospital) ;
  • Sarma, Manash P (Department of Biotechnology, Assam Down Town University) ;
  • Medhi, Subhash (Department of Bioengineering and Technology, Laboratory of Molecular Virology and Oncology, Gauhati University Institute of Science and Technology, Gauhati University)
  • Published : 2016.01.11
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Abstract

Background: To investigate polymorphisms in heat shock proteins A1B and A1L (HOM) and associated risk of oesophageal carcinoma in Northeast India. Materials and Methods: The study includes oesophageal cancer (ECA) patients attending general outpatient department (OPD) and endoscopic unit of Gauhati Medical College. Patients were diagnosed based on endoscopic and histopathological findings. Genomic DNA was typed for HSPA1B1267 and HSPA1L2437 SNPs using the polymerase chain reaction with restriction fragment length polymorphisms. Results: A total of 78 cases and 100 age-sex matched healthy controls were included in the study with a male: female ratio of 5:3 and a mean age of $61.4{\pm}8.5years$. Clinico-pathological evaluation showed 84% had squamous cell carcinoma and 16% were adenocarcinoma. Dysphagia grades 4 (43.5%) and 5 (37.1%) were observed by endoscopic and hispathological evaluation. The frequency of genomic variation of A1B from wild type A/A to heterozygous A/G and mutant G/G showed a positive association [chi sq=19.9, p=<0.05] and the allelic frequency also showed a significant correlation [chi sq=10.3, with cases vs. controls, OR=0.32, $p{\leq}0.05$]. The genomic variation of A1L from wild T/T to heterozygous T/C and mutant C/C were found positively associated [chi sq=7.02, p<0.05] with development of ECA. While analyzing the allelic frequency, there was no significant association [chi sq=3.19, OR=0.49, p=0.07]. Among all the risk factors, betel quid [OR=9.79, Chi square=35.0, p<0.05], tobacco [OR=2.95, chi square=10.6, p<0.05], smoking [OR=3.23, chi square=10.1, p<0.05] demonstrated significant differences between consumers vs. non consumers regarding EC development. Alcohol did not show any significant association [OR=1.34, chi square=0.69, p=0.4] independently. Conclusions: It can be concluded that the present study provides marked evidence that polymorphisms of HSP70 A1B and HSP70 A1L genes are associated with the development of ECA in a population in Northeast India, A1B having a stronger influence. Betel quid consumption was found to be a highly significant risk factor, followed by smoking and tobacco chewing. Although alcohol was not a potent risk factor independently, alcohol consumption along with tobacco, smoking and betel nut was found to contribute to development of ECA.

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References

  1. Akhtar S, Sheikh AA, Qureshi HU (2012). Chewing areca nut, betel quid, oral snuff, cigarette smoking and the risk of oesophageal squamous-cell carcinoma in South Asians: a multicentre case-control study. Eur J Cancer, 48, 655-61. https://doi.org/10.1016/j.ejca.2011.06.008
  2. Asea A, Kraeft SK, Kurt-Jones EA, et al (2000). HSP70 stimulates cytokine production through a CD14-dependant pathway, demonstrating its dual role as a chaperone and cytokine. Nat Med, 6, 435-42. https://doi.org/10.1038/74697
  3. Boiocchi C, Osera C, Monti MC, et al (2014). Are Hsp70 protein expression and genetic polymorphism implicated in multiple sclerosis inflammation? J Neuroimmunol, 268, 84-8. https://doi.org/10.1016/j.jneuroim.2014.01.004
  4. Chen J, Zhang N, Ling Y, et al (2011). Alcohol consumption as a risk factor for oesophageal adenocarcinoma in North China. Exp Med, 224, 21-7.
  5. Chitra S, Ashok L, Anand L, et al (2004). Risk factors for esophageal cancer in Coimbatore, southern India: a hospitalbased case-control study. Indian J Gastroenterol, 23, 19-21.
  6. Chouchane L, Ahmed SB, Baccouche S, et al (1997). Polymorphism in the tumor necrosis factor-alpha promotor region and in the heat shock protein 70 genes associated with malignant tumors. Cancer, 80, 1489-96. https://doi.org/10.1002/(SICI)1097-0142(19971015)80:8<1489::AID-CNCR17>3.0.CO;2-1
  7. Daugaard M, Rohde M, Jaattela M (2007). The heat shock protein 70 family: Highly homologous proteins with overlapping and distinct functions. FEBS Lett, 581, 3702-10. https://doi.org/10.1016/j.febslet.2007.05.039
  8. Dar NA, Bhat GA, Shah IA, Iqbal B, et al (2012). Hookah smoking, nass chewing, and oesophageal squamous cell carcinoma in Kashmir, India. Br J Cancer. 107, 1618-23. https://doi.org/10.1038/bjc.2012.449
  9. Favatier F, Bornman L, Hightower LE, et al (1997). Variation in hsp gene expression and Hsp polymorphism: do they contribute to differential disease susceptibility and stress tolerance? Cell Stress Chaperones, 2, 141-55. https://doi.org/10.1379/1466-1268(1997)002<0141:VIHGEA>2.3.CO;2
  10. Fekete A, Viklicky O, Hubacek JA, et al (2006). Association between heat shock protein 70s and toll-like receptor polymorphisms with long-term renal allograft survival. Transpl Int. 19, 190-6. https://doi.org/10.1111/j.1432-2277.2006.00265.x
  11. Ferrer-Ferrer M, Malespin-Bendana W, Ramirez V, et al (2013). Polymorphisms in genes coding for HSP-70 are associated with gastric cancer and duodenal ulcer in a population at high risk of gastric cancer in Costa Rica. Arch Med Res, 44, 467-74. https://doi.org/10.1016/j.arcmed.2013.08.008
  12. Ganesh B, Talole SD, Dikshit R (2009). Tobacco, alcohol and tea drinking as riskfactors for esophageal cancer: A case-control study from Mumbai, India. Cancer Epidemiol, 33, 431-4. https://doi.org/10.1016/j.canep.2009.09.002
  13. Giri PA, Singh KK, Phalke DB (2014). Study of sociodemographic determinants ofesophageal cancer at a tertiary care teaching hospital of Western Maharashtra,India. South Asian J Cancer, 3, 54-6. https://doi.org/10.4103/2278-330X.126526
  14. Gopala Krishnappa BR, Vijay CR, Ramesh C, et al (2013). Trends in oesophagus and Stomach cancer incidence in Bangalore, India Gulf J Oncol, 1, 42-50.
  15. Holmes RS, Vaughan TL (2007). Epidemiology and pathogenesis of esophageal cancer. Semin Radiat Oncol, 17, 2-9. https://doi.org/10.1016/j.semradonc.2006.09.003
  16. Hanahan D, Weinberg RA (2011). Hallmarks of cancer: the next generation. Cell, 144, 646-74. https://doi.org/10.1016/j.cell.2011.02.013
  17. Ke L (2002). Mortality and incidence trends from esophagus cancer in selectedgeographic areas of China circa 1970-90. Int J Cancer, 102, 271-4. https://doi.org/10.1002/ijc.10706
  18. Kuang D, Chen W, Song YZ, et al., 2014. Association between the HSPA1B ${\pm}$1267A/G polymorphism and cancer risk: a meta-analysis of 14 case-control studies. Asian Pac J Cancer Prev, 15, 6855-61. https://doi.org/10.7314/APJCP.2014.15.16.6855
  19. Kummee P, Tangkijvanich P, Poovorawan Y, et al (2007). Association of HLA-DRB1*13 and TNF-alpha gene polymorphisms with clearance of chronic hepatitis B infection and risk of hepatocellular carcinoma in Thai population. J Viral Hepat, 14, 841-8.
  20. Layke JC, Lopez PP (2006). Esophageal cancer: a review and update. Am Fam Physician, 73, 2187-94.
  21. Lee CH, Lee JM, Wu DC, et al (2005). Independent and combined effects of alcohol intake, tobacco smoking and betel quid chewing on the risk of esophageal cancer in Taiwan. Int J Cancer, 113, 475-82. https://doi.org/10.1002/ijc.20619
  22. Liu J, Cheng J, Peng J, et al (2007). Effects of polymorphisms of heatshock protein 70 gene on ischemic stroke, and interaction with smoking in China. Clin Chim Acta, 384, 64-8. https://doi.org/10.1016/j.cca.2007.05.021
  23. Lovett MC, Coates JR, Shu Y, et al (2014). Quantitative assessment of hsp70, IL-1${\beta}$ and TNF-$\alpha$ in the spinal cord of dogs with E40K SOD1-associated degenerative myelopathy. Vet J, 200, 312-7. https://doi.org/10.1016/j.tvjl.2014.03.003
  24. Matokanovic M, Rumora L, Popovic-Grle S, et al., (2012). Association of hsp70-2 (+1267A/G), hsp70-hom (+2437T/C), HMOX-1 (number of GT repeats) and TNF-alpha (+489G/A) polymorphisms with COPD in Croatian population. Clin Biochem, 45, 770-4. https://doi.org/10.1016/j.clinbiochem.2012.04.003
  25. Medhi S, Sarma MP, Asim M, et al (2013). Genetic variants of heat shock proteinA1L2437 and A1B1267 as possible risk factors for hepatocellular carcinoma in India. J Viral Hepat, 20, 141-7. https://doi.org/10.1111/j.1365-2893.2012.01639.x
  26. Meimaridou E, Gooljar SB, Chapple JP (2009). From hatching to dispatching: themultiple cellular roles of the Hsp70 molecular chaperone machinery. J Mol Endocrinol, 42, 1-9.
  27. Mestiri S, Bouaouina N, Ahmed SB, Khedhaier, et al (2001). Genetic variation in the tumor necrosis factor-alpha promoter region and in the stress protein hsp70-2: susceptibility and prognostic implications in breast carcinoma. Cancer, 91, 672-8. https://doi.org/10.1002/1097-0142(20010215)91:4<672::AID-CNCR1050>3.0.CO;2-J
  28. Milner RE, Busaan J, Michalak M (1992) Isolation and characterization of different C-terminal fragments of dystrophin expressed in Escherichia coli. Biochem J, 288, 1037-44. https://doi.org/10.1042/bj2881037
  29. Kurkalang S, Banerjee A, Dkhar H, et al (2015). Precocious anaphase and expression of Securin and p53 genes as candidate biomarkers for the early detection in areca nutinduced carcinogenesis. Mutagenesis, 30, 381-9. https://doi.org/10.1093/mutage/geu083
  30. Pandeya N, Williams G, Green AC, et al (2009). Australian Cancer Study. Alcohol consumption and the risks of adenocarcinoma and squamous cell carcinoma of the esophagus. Gastroenterol, 136, 1215-24, https://doi.org/10.1053/j.gastro.2008.12.052
  31. Phukan RK, Ali MS, Chetia CK, et al (2001). Betel nut and tobacco chewing; potential risk factors of cancer of oesophagus in Assam, India. Br J Cancer, 85, 661-7. https://doi.org/10.1054/bjoc.2001.1920
  32. Prabhu A, Obi KO, Rubenstein JH (2013). Systematic review with meta-analysis:race-specific effects of alcohol and tobacco on the risk of oesophageal squamous cell carcinoma. Aliment Pharmacol Ther, 38, 1145-55. https://doi.org/10.1111/apt.12514
  33. Rohde M, Daugaard M, Jensen MH, et al (2005). Members of the heat-shock protein 70 family promote cancer cell growth by distinct mechanisms. Genes Dev, 19, 570-82. https://doi.org/10.1101/gad.305405
  34. Sambrook J, Fritsch EF, Maniatis T (1990). Molecular cloning: a laboratory manual, 2nd edn. cold spring harbor, NY, USA: Cold Spring Harbor Laboratory Press, 73.
  35. Szondy K, Rusai K, Szabo AJ, et al (2012). Tumor cell expression of heat shock protein (HSP) 72 is influenced by HSP72 [HSPA1B A(1267)G] polymorphism and predicts survival in small Cell lung cancer (SCLC) patients. Cancer Invest, 30, 317-22. https://doi.org/10.3109/07357907.2012.657815
  36. Vabulas RM, Ahmad-Nejad P, Ghose S, et al (2002). HSP70 as endogenous stimulus of the Toll/interleukin-1 receptor signal pathway. J Biol Chem, 277, 15107-12. https://doi.org/10.1074/jbc.M111204200
  37. Vargas-Alarcon G, Londono JD, Hernandez-Pacheco G, et al (2002). Heat shock protein 70 gene polymorphisms in Mexican patients with spondyloarthropathies. Ann Rheum Dis, 61, 48-51. https://doi.org/10.1136/ard.61.1.48
  38. Waters JS, Tait D, Cunningham D, et al (2002). A multicentre phase II trial of primary chemotherapy with cisplatin and protracted venous infusion 5-fluorouracil followed by chemoradiation in patients with carcinoma of the oesophagus. Ann Oncol, 13, 1763-70. https://doi.org/10.1093/annonc/mdf301
  39. Wang J, Qiu M, Xu Y, et al (2015). Long noncoding RNA CCAT2 correlates with smoking in esophageal squamous cell carcinoma. Tumour Biol, 36, 5523-8. https://doi.org/10.1007/s13277-015-3220-x
  40. Wang XP, Qiu FR, Liu GZ, et al (2005). Correlation between clinicopathology andexpression of heat shock protein 70 and glucose-regulated protein 94 in humancolonic adenocarcinoma. World J Gastroenterol, 11, 1056-9. https://doi.org/10.3748/wjg.v11.i7.1056
  41. Wu YR, Wang CK, Chen CM, et al (2004). Analysis of heatshock protein 70 gene polymorphisms and the risk of Parkinson's disease. Hum Genet, 114, 236-41. https://doi.org/10.1007/s00439-003-1050-1
  42. Yang SJ, Yokoyama A, Yokoyama T, et al (2010). Relationship between genetic polymorphisms of ALDH2 andADH1B and esophageal cancer risk: a meta-analysis. World J Gastroenterol, 16, 4210-20. https://doi.org/10.3748/wjg.v16.i33.4210
  43. Yang CX, Wang HY, Wang ZM, et al (2005). Risk factors for esophageal cancer: a case-control study in South-western China. Asian Pac J Cancer Prev, 6, 48-53.
  44. Zhang HY, Spechler SJ, Souza RF (2009). Esophageal adenocarcinoma arising in Barrett esophagus. Cancer Lett, 275, 170-7. https://doi.org/10.1016/j.canlet.2008.07.006
  45. Matokanovic M, Rumora L, Popovic-Grle S, et al (2012). Association of hsp70-2 (+1267A/G), hsp70-hom (+2437T/C), HMOX-1 (number of GT repeats) and TNF-alpha (+489G/A) polymorphisms with COPD in Croatian population. Clin Biochem, 45, 770-4. https://doi.org/10.1016/j.clinbiochem.2012.04.003