한국축산시설환경학회지 (Journal of Animal Environmental Science)

한국축산환경학회 (The Korean Society of Animal Environmental Science & Technology)
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Next Generation Sequencing을 통한 미생물 군집 분석의 축산분야 활용

Application of Next Generation Sequencing to Investigate Microbiome in the Livestock Sector

  • 김민석 (농촌진흥청 국립축산과학원) ;
  • 백열창 (농촌진흥청 국립축산과학원) ;
  • 오영균 (농촌진흥청 국립축산과학원)
  • Kim, Minseok (Animal Nutrition and Physiology Team, National Institute of Animal Science) ;
  • Baek, Youlchang (Animal Nutrition and Physiology Team, National Institute of Animal Science) ;
  • Oh, Young Kyoon (Animal Nutrition and Physiology Team, National Institute of Animal Science)
  • 투고 : 2015.07.09
  • 심사 : 2015.08.12
  • 발행 : 2015.09.30
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초록

The objective of this study was to review application of next-generation sequencing (NGS) to investigate microbiome in the livestock sector. Since the 16S rRNA gene is used as a phylogenetic marker, unculturable members of microbiome in nature or managed environments have been investigated using the NGS technique based on 16S rRNA genes. However, few NGS studies have been conducted to investigate microbiome in the livestock sector. The 16S rRNA gene sequences obtained from NGS are classified to microbial taxa against the 16S rRNA gene reference database such as RDP, Greengenes and Silva databases. The sequences also are clustered into species-level OTUs at 97% sequence similarity. Microbiome similarity among treatment groups is visualized using principal coordinates analysis, while microbiome shared among treatment groups is visualized using a venn diagram. The use of the NGS technique will contribute to elucidating roles of microbiome in the livestock sector.

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참고문헌

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