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Analysis of phenotypic characterization of segregation population developed by crossing in Flammulina velutipes

팽이버섯(Flammulina velutipes) 교잡분리집단의 형태학적 특성 분석

  • Woo, Sung-I (Department of Applied Biology Graduate School, Kangwon National University) ;
  • Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Eun-Seou (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Jang, Kab yeul (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Shin, Pyung-Gyun (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Oh, Youn-Lee (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Oh, Min ji (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Nam, Youn-keol (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Kyung-Soo (Department of Applied Biology Graduate School, Kangwon National University)
  • 우성이 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 공원식 (강원대 응용생물학과) ;
  • 김은선 (강원대 응용생물학과) ;
  • 장갑열 (강원대 응용생물학과) ;
  • 신평균 (강원대 응용생물학과) ;
  • 오연이 (강원대 응용생물학과) ;
  • 오민지 (강원대 응용생물학과) ;
  • 남윤걸 (강원대 응용생물학과) ;
  • 김경수 (농촌진흥청 국립원예특작과학원 버섯과)
  • Received : 2015.09.07
  • Accepted : 2015.09.20
  • Published : 2015.09.30

Abstract

This study was conducted to obtain a growth correlation of basal information from the development of disease resistant Flammulina velutipes cultivars through back-crossing between the strains of wild-type brown monokaryon 4019-20 and the derivative of commercial quality white monokaryons 3. The two strains were selected to back-cross for further enhancing their latent attributes and growth characteristics. The parents of 4019-$20{\times}M3$ back-crossed to reproduce $F_1$, M3-Sn. Using $F_1$, M3-Sn procured and isolated into 94 monokaryon strains. Further examination of growth characteristics carried out by back-crossing between M3 and $BC_1F_1$ from M3-n dikaryon. Monokaryon exhibited an irregular growth pattern and demonstrated to be sluggish development in the sawdust medium. However $BC_1F_1$(M3-n) dikaryon strains confirmed mostly regular growth pattern and demonstrated ordinary growth in the sawdust medium. The fruitbody of $BC_1F_1$ confirmed as light-brown colour to be the dominant gene. The colour distributions of fruitbody, $BC_1F_1$, resulted as follows; 7% of dark brown, 25% of brown, 27% of light brown, 16% of ivory and 25% of white. The ratio of the other color to white showed 3 to 1 which suggested two major genes were related to fruitbody color.

팽이버섯의 야생 갈색 균주에서 유래한 단핵 계통 ASI 4019-20과 상용 품질에서 유래한 백색 단핵균주 M3를 교잡하여 분리집단을 만들고 그 특성을 조사하여 육종의 기초 자료를 작성하였다. 4019-$20{\times}M3$ 교잡으로 육성된 $F_1$인 M3-Sn 계통으로부터 단핵균주 94개를 분리하고 다시 교배 모본의 하나인 M3와 교잡하여 $BC_1F_1$인 M3-n 집단을 육성하여 생장특성을 조사하였다. 분리된 단핵균주는 형태상으로는 균사의 생장 형태가 불규칙한 것이 있었으며 톱밥 생장이 늦은 것이 많았다. 그 반면 이핵균주인 $BC_1F_1$(M3-n) 계통의 균사는 생장형태가 대부분 균일하며 톱밥에서 생장이 양호하였다. 갈색 야생 균주와 백색 상용 품종 유래의 단핵균주간 교잡으로 육성된 $F_1$의 자실체 갓색은 연갈색으로 나타나 유색이 백색에 우성이었으며, 백색 회보친을 교잡하여 육성한 $BC_1F_1$ 분리집단에서 색깔이 분포는 진갈색 7%, 갈색 25%, 연한갈색 27%, 아이보리 16%, 흰색 25%로 나타나 전체 유색에 백색의 비율은 3:1이었다.

Keywords

References

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