Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Physiological activitive of Grifola frondosa by log cultivation and bottle cultivation

원목재배와 병재배 잎새버섯(Grifola frondosa)의 생리활성

  • Kim, Jong-Bong (Department of medical life Science, Catholic University of Daegu) ;
  • Seo, Hye-Young (Department of medical life Science, Catholic University of Daegu)
  • 김종봉 (대구가톨릭대학교 의생명과학과) ;
  • 서혜영 (대구가톨릭대학교 의생명과학과)
  • Received : 2015.08.10
  • Accepted : 2015.09.05
  • Published : 2015.09.30
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Abstract

This research was carried out to determine the differences of physiological activites between Grifola frondosa of log cultivation(LC) and Grifola frondosa of bottle cultivation(BC). Total flavonoids content, total phenolics content, electron donating ability(EDA), nitrite-scavenging ability(NSA), SOD-like activity and inhibitory effect of Xanthine oxidase were examined. The highest value of total flavonoid content is $5.96{\pm}0.81mg/g$ in water extract from Grifola frondosa of log cultivation at $40^{\circ}C$ (LC-W40) but, one of total phenolics compound is $44.53{\pm}0.89mg/g$ in water extract from Grifola frondosa of bottle cultivation at $40^{\circ}{\cdots}$ (BC-W40). The EDA using DPPH of BC-W40 extract showed the highest value of $97.14{\pm}0.71%$. Nitrite-scavenging ability was $62.55^{\circ}{\ae}0.36%$ in extract from Grifola frondosa of BC-W40 at pH 1.2. The value was SOD-like activity showed the highest value of $18.95^{\circ}{\ae}1.39%$ in extract from LC-W40. Xanthine oxidase inhibitory activity was the highest value of $54.31{\pm}0.40%$ in extract from Grifola frondosa BC-W40, and dependent on concentrations. These results showed that a the antioxidant effects of Grifola frondosa is excellent. However, physiological activities of Grifola frondosa were not depend on caltivation method regulary, and were different according to kind of solvents, concentraitions and physialogical factors examined such as EDA, SOD-like activity and NSA.

본 연구에서는 잎새버섯에서 병재배와 원목재배에 따른 생리활성의 차이가 있는지를 밝히기 위하여 수행하였다. 총 플라보노이드 함량, 총 페놀 함량, 전자공여능, 아질산염소거능, SOD 유사활성, Xanthine oxidase 저해 등을 분석하였다. 총 플라보노이드함량은 $40^{\circ}C$ 물에서 추출한 원목재배 잎새버섯의 $5.96{\pm}0.81mg/g$이 가장 높았고 총 페놀함량은 $40^{\circ}C$ 물에서 추출한 병재배버섯의 $44.53{\pm}0.89$mg/g이 가장 높았다. DPPH를 이용하여 측정한 전자공여능은 BC-W40 잎새버섯 추출물의 $97.14{\pm}0.71%$가 가장 높았다. 아질산염소거능은 pH 1.2에서 BC-W40 잎새버섯 추출한 것의 $62.55{\pm}0.36%$가 가장 높았다. 가장 높은 SOD 유사활성은 LC-W40로부터 추출한 추출물의 $18.95{\pm}1.39%$였다. 잎새버섯의 Xanthine 저해 효과는 BC-W40 추출물 $54.13{\pm}0.40%$가 가장 높았고 그 효과는 농도에 비례하였다. 이상의 결과들은 잎새버섯이 우수한 항산화 기능이었음을 보여주었다. 그러나 잎새버섯의 생리활성들은 재배 방법에 따라 일정하지는 않았고 용매의 종류, 농도 및 전자공여능, SOD 유사활성도, 아질산소거능 등과 같은 생리작용 요인들에 따라 달랐다.

Keywords

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