Journal of Acupuncture Research

  • 제32권3호
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  • Pages.41-51
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  • 2015
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  • 2586-288X(pISSN)
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  • 2586-2898(eISSN)
대한침구의학회 (Korean Acupuncture & Moxibustion Medicine Society)
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태충·삼음교의 침 자극이 Streptozotocin으로 유발된 당뇨쥐의 신장 손상에 미치는 영향

The Effects of LR3 and SP6 Acupuncture on Renal Damage in Streptozotocin-induced Diabetic Mice

  • 이초인 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이현종 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이윤규 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 임성철 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 김재수 (대구한의대학교 한의과대학 침구경혈학교실)
  • Lee, Cho In (Department of Acupuncture & Moxibustion, Meridian & Acupoint, College of Oriental Medicine, Daegu Haany University) ;
  • Lee, Hyun Jong (Department of Acupuncture & Moxibustion, Meridian & Acupoint, College of Oriental Medicine, Daegu Haany University) ;
  • Lee, Yun Kyu (Department of Acupuncture & Moxibustion, Meridian & Acupoint, College of Oriental Medicine, Daegu Haany University) ;
  • Lim, Seong Chul (Department of Acupuncture & Moxibustion, Meridian & Acupoint, College of Oriental Medicine, Daegu Haany University) ;
  • Kim, Jae Soo (Department of Acupuncture & Moxibustion, Meridian & Acupoint, College of Oriental Medicine, Daegu Haany University)
  • 투고 : 2015.07.31
  • 심사 : 2015.09.09
  • 발행 : 2015.09.20
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초록

Objectives : This study was performed to investigate the effects of $LR_3$ and $SP_6$ acupuncture on renal damage in streptozotocin(STZ)-induced diabetic mice. Methods : ICR male mice were stabilized for a week and divided into four groups: a normal mice group(N), no-acupuncture diabetic mice group(Control), $LR_3$ acupuncture diabetic mice group($LR_3$), and $SP_6$ acupuncture diabetic mice group($SP_6$). Diabetes was experimentally induced by intraperitoneal injection of STZ(150 mg/kg) in citrate buffer(pH 4.5). For two weeks, $LR_3$ and $SP_6$ acupunctures were administered bilaterally at each point once a day. After two weeks, the animals' weight was measured and they underwent a laparotomy. Serum glucose and blood urea nitrogen(BUN) were measured from the blood taken from the heart. We measured glucose, reactive oxygen species(ROS), peroxynitrite($ONOO^-$) and thiobarbituric acid reactive substances(TBARS) in the kidney and compared expression levels of superoxide dismutases(SOD), glutathione peroxidase(GPx), nuclear factor-kappa B(NF-${\kappa}B$), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase(iNOS) and Interleukin-1 beta(IL-$1{\beta}$). Results : BUN significantly decreased in $LR_3$, $SP_6$ compared to the control group. $LR_3$ showed significantly decreased glucose compared to the control group. $LR_3$, $SP_6$ significantly decreased in ROS and $ONOO^-$ compared to the control group. $LR_3$ significantly decreased in TBARS compared to the control group. $SP_6$ significantly increased in expressions of SOD-1, catalase, and GPx compared to the control group. $LR_3$, $SP_6$ significantly decreased in COX-2 compared to the control group. $SP_6$ significantly decreased in IL-$1{\beta}$ compared to the control group. Conclusions : This study suggests that $LR_3$ acupuncture may be effective in controlling glucose and lipid peroxidation and that $SP_6$ acupuncture may have anti-oxidative and anti-inflammatory effects on renal damage in STZ-induced diabetic mice.

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