Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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The Effect of Pyrrosiae Herba Herbal-acupuncture at KI10 on Lipopolysaccharide Induced Nephritis in Rats

음곡에 시술한 석위약침이 Lipopolysaccharide로 유도된 흰쥐의 신장염에 미치는 영향

  • Jang, Seung Hoon (Department of Meridian & Acupoint, College of Korean Medicine, Daejeon University) ;
  • Kim, Jea Hong (Department of Meridian & Acupoint, College of Korean Medicine, Daejeon University) ;
  • Yim, Yun Kyoung (Department of Meridian & Acupoint, College of Korean Medicine, Daejeon University)
  • 장승훈 (대전대학교 한의과대학 경락경혈학교실) ;
  • 김재홍 (대전대학교 한의과대학 경락경혈학교실) ;
  • 임윤경 (대전대학교 한의과대학 경락경혈학교실)
  • Received : 2015.06.01
  • Accepted : 2015.08.25
  • Published : 2015.09.20
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Abstract

Objectives : The purpose of this study is to investigate the effects of Pyrrosiae Herba herbal-acupuncture(PH-HA) at $KI_{10}$(Umgok) on nephritis induced by lipopolysaccharide(LPS) in rats. Methods : Rats were assigned to four groups: normal, LPS, saline and PH-HA. Rats in the saline and PH-HA groups were treated with saline injection and PH-HA respectively at $KI_{10}$, three times over the period of one week. All animals, except those in the normal group, were injected intra-peritoneally with LPS to induce nephritis. WBC, in blood, tumor necrosis factor alpha(TNF-${\alpha}$), cytokine-induced neutrophil chemoattractant 1(CINC-1), blood urea nitrogen(BUN), creatinine in serum, urinal volume, total protein creatinine in urine, and renal myeloperoxidase (MPO) were analyzed. Results : 1. PH-HA group showed significantly reduced levels of serum BUN, serum creatinine, TNF-${\alpha}$, and CINC-1 compared to the LPS group. Furthermore, a significant increase in urine output and more significant decreases in total protein in urine and MPO in renal tissue were observed in the PH-HA group when compared to the LPS group. 2. The PH-HA group showed significantly reduced levels of serum creatinine and renal MPO, and a more significant increase in urine output compared to the saline group. Conclusions : According to these results, it is postulated that PH-HA at $KI_{10}$ has anti-inflammatory and renal-protective effects on LPS-induced nephritis in rats, and both acupoint $KI_{10}$ and the herb Pyrrosiae Herba made contributions to these effects. Further studies on the interaction between acupoint $KI_{10}$ and the herb Pyrrosiae Herba may be needed.

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