대한방사성의약품학회지 (Journal of Radiopharmaceuticals and Molecular Probes)
- 제1권2호
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- Pages.118-122
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- 2015
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- 2384-1583(pISSN)
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- 2508-3848(eISSN)
DOI QR Code
Suicidal gene therapy with rabbit cytochrome P450 4B1/2-aminoanthracene or 4-ipomeanol system in human colon cancer cell
- Jang, Su Jin (Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS)) ;
- Kang, Joo Hyun (Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS)) ;
- Moon, Byung Seok (Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seoul National University College of Medicine) ;
- Lee, Yong Jin (Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS)) ;
- Kim, Kwang Il (Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS)) ;
- Lee, Tae Sup (Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS)) ;
- Choe, Jae Gol (Department of Nuclear Medicine, Korea University Anam Hospital, Korea University College of Medicine) ;
- Lim, Sang Moo (Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS))
- 투고 : 2015.11.11
- 심사 : 2015.12.19
- 발행 : 2015.12.30
초록
Suicidal gene therapy is based on the transduction of tumor cells with "suicide" genes encoding for prodrug-activating enzymes that render target cells susceptible to prodrug treatment. Suicidal gene therapy results in the death of tumor with the expression of gene encoding enzyme that converts non-toxic prodrug into cytotoxic product. Cytochrome P450 4B1 (CYP4B1) activates 4-ipomeanol (4-IPO) or 2-aminoanthracene (2-AA) to cytotoxic furane epoxide and unsaturated dialdehyde intermediate.In this study, therapeutic effects of suicidal gene therapy with rabbit CYP4B1/2-AA or 4-IPO system were evaluated in HT-29 (human colon cancer cell). pcDNA-CYP4B1 vector was transfected into HT-29 by lipofection and stable transfectant was selected by treatment of hygromycin (