Asian Pacific Journal of Cancer Prevention

  • 제16권16호
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  • Pages.7261-7266
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  • 2015
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Shikonin Induced Necroptosis via Reactive Oxygen Species in the T-47D Breast Cancer Cell Line

  • Shahsavari, Zahra (Cancer Research Laboratory, Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University) ;
  • Karami-Tehrani, Fatemeh (Cancer Research Laboratory, Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University) ;
  • Salami, Siamak (Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences)
  • 발행 : 2015.11.04
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초록

Breast cancer, the most common cancer in the women, is the leading cause of death. Necrotic signaling pathways will enable targeted therapeutic agents to eliminate apoptosis-resistant cancer cells. In the present study, the effect of shikonin on the induction of cell necroptosis or apoptosis was evaluated using the T-47D breast cancer cell line. The cell death modes, caspase-3 and 8 activities and the levels of reactive oxygen species (ROS) were assessed. Cell death mainly occurred through necroptosis. In the presence of Nec-1, caspase-3 mediated apoptosis was apparent in the shikonin treated cells. Shikonin stimulates ROS generation in the mitochondria of T-47D cells, which causes necroptosis or apoptosis. Induction of necroptosis, as a backup-programmed cell death pathway via ROS stimulation, offers a new strategy for the treatment of breast cancer.

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참고문헌

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  3. The cytotoxic effects of propolis on breast cancer cells involve PI3K/Akt and ERK1/2 pathways, mitochondrial membrane potential, and reactive oxygen species generation pp.1568-5608, 2018, https://doi.org/10.1007/s10787-018-0492-y
  4. Shikonin inhibits cancer cell cycling by targeting Cdc25s vol.19, pp.1, 2019, https://doi.org/10.1186/s12885-018-5220-x