Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Tobacco Use Increases Oxidative DNA Damage in Sperm - Possible Etiology of Childhood Cancer

  • Kumar, Shiv Basant (Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Sciences) ;
  • Chawla, Bhavna (Ocular Oncology & Pediatric Ophthalmology Service, Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences) ;
  • Bisht, Shilpa (Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Sciences) ;
  • Yadav, Raj Kumar (Integral Health Clinics, Department of Physiology, All India Institute of Medical Sciences) ;
  • Dada, Rima (Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Sciences)
  • Published : 2015.11.04
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Abstract

Background: Cigarette smoking and tobacco chewing are common modes of consuming tobacco all over the world. Parents need to be aware that germ cell integrity is vital for birth of healthy offspring as biological parenting begins much before birth of a child and even before conception. The present study was conducted to determine the etiology of non-familial sporadic heritable retinoblastoma (NFSHRb), by evaluating oxidative sperm DNA damage in fathers due to use of tobacco (smoking and chewing). Materials and Methods: We recruited 145 fathers of NFSHRb children and 53 fathers of healthy children (controls) in the study. Tobacco history was obtained by personal interview. Seminal reactive oxygen species (ROS) in semen, sperm DNA fragmentation index (DFI) and 8 hydroxy 2' deoxyguanosine (8-OHdG) levels in sperm were evaluated. The RB1 gene was screened in genomic blood DNA of parents of children with NFSHRb and controls. Odds ratios (ORs) derived from conditional logistic regression models. Results: There was significant difference in the levels of ROS (p<0.05), DFI (p<0.05) and 8-OHdG (p<0.05) between tobacco users and non-users. The OR of NFSHRb for smokers was 7.29 (95%CI 2.9-34.5, p<0.01), for tobacco chewers 4.75 (2.07-10.9, p<0.05) and for both 9.11 (3.79-39.2; p<0.01). Conclusions: This study emphasizes the adverse effect of tobacco on the paternal genome and how accumulation of oxidative damage in sperm DNA may contribute to the etiology of NFSHRb. In an ongoing parallel study in our laboratory, 11 of fathers who smoked underwent. Meditation and yoga interventions, showed significant decline in levels of highly mutagenic oxidised DNA adducts after 6 months. Thus our lifestyle and social habits impact sperm DNA integrity and simple interventions like yoga and meditation are therapeutic for oxidative damage to sperm DNA.

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References

  1. Aitken RJ, Smith TB, Jobling MS, et al (2014). Oxidative stress and male reproductive health. Asian J Androl, 16, 31-8. https://doi.org/10.4103/1008-682X.122203
  2. Attia SM, Ahmad SF, Okash RM, et al (2014). Aroclor 1254-induced genotoxicity in male gonads through oxidatively damaged DNA and inhibition of DNA repair gene expression. Mutagenesis, 29, 379-84. https://doi.org/10.1093/mutage/geu029
  3. de Paula WB, Lucas CH, Agip AN, et al (2013). Energy, ageing, fidelity and sex: oocyte mitochondrial DNA as a protected genetic template. Philos Trans R Soc Lond B Biol Sci, 368, 20120263. https://doi.org/10.1098/rstb.2012.0263
  4. Dinham B and Malik S (2003). Pesticide and human rights. Into J Occupy Environ Health, 9, 40-52. https://doi.org/10.1179/oeh.2003.9.1.40
  5. Evenson DP and Wixon R (2005). Environmental toxicants cause sperm DNA fragmentation as detected by the sperm chromatin structure assay (SCSA). Toxicol Appl Pharmacol, 207, 532-37. https://doi.org/10.1016/j.taap.2005.03.021
  6. Evenson DP and Wixon R (2006). Clinical aspects of sperm DNA fragmentation detection and male infertility. Theriogenol, 65, 979-91. https://doi.org/10.1016/j.theriogenology.2005.09.011
  7. Feinberg JI, Bakulski KM, Jaffe AE, et al (2015). Paternal sperm DNA methylation associated with early signs of autism risk in an autism-enriched cohort. Int J Epidemiol, 0, 1-12.
  8. GATS India report (2014). Ministry of Health and Family Welfare. URL: http://www.mohfw.nic.in/WriteReadData/l892s/1455618937GATS%20 India.
  9. Gray LE, Ostby J, Furr J, et al (2001). Effects of environmental antiandrogens on reproductive development in experimental animals. Human Reproduction Update, 2, 248-64.
  10. Hammond D, Costello MJ, Fong GT, et al (2006). Exposure to tobacco marketing and support for tobacco control policies. Am J Health Behav, 30, 700-9. https://doi.org/10.5993/AJHB.30.6.15
  11. Haoyang L, Xinzhou L, Yulin D, et al (2013). DNA methylation, a hand behind neurodegenerative diseases. Front Aging Neurosci, 5, 85.
  12. Hazarika M, Krishnatreya M, Bhuyan C, et al (2014). Overview of childhood cancers at a regional cancer centre in North-East India. Asian Pac J Cancer Prev, 15, 7817-9. https://doi.org/10.7314/APJCP.2014.15.18.7817
  13. Jeng HA (2014). Exposure to endocrine disrupting chemicals and male reproductive health. Front Public Health, 2, 55.
  14. Khan MM (2005). Suicide prevention and developing countries. J R Soc Med, 98, 459-3. https://doi.org/10.1258/jrsm.98.10.459
  15. Kumar K, Thilagavathi J, Deka D, et al (2012). Unexplained early pregnancy loss:role of paternal DNA. Indian J Med Res, 136, 296-98.
  16. Kumar SB, Chawla B, Seth R, et al (2014). Loss of paternal sperm DNA integrity in non- familial retinoblastoma. IOVS, 55, 6440.
  17. Kumar SB, Gautam S, Tolahunase M, et al (2015). Improvement in sperm DNA quality following simple life style intervention: a study in fathers of children with non-familial sporadic heritable retinoblastoma. J Clin Case Rep, 5, 509.
  18. Kumar SB, Yadav R, Yadav RK, et al (2015). Telomerase activity and cellular aging might be positively modified by a yoga-based lifestyle intervention. J Altern Complement Med, 21, 370-2. https://doi.org/10.1089/acm.2014.0298
  19. Meel R, Radhakrishnan V, Bakhshi S (2012). Current therapy and recent advances in the management of retinoblastoma. Indian J Med Paediatr Onco, 33, 80-8. https://doi.org/10.4103/0971-5851.99731
  20. Mishra SS, Kumar R, Malhotra N, et al.,(2013) Expression of PARP1 in primary infertility patientsandcorrelation with DNA fragmentation index a pilot study. J Anat Soc India, 62, 98-104. https://doi.org/10.1016/j.jasi.2013.12.005
  21. Niu ZH, Liu JB, Shi TY, et al (2010). Impact of cigarette smoking on human sperm DNA integrity. Zhonghua Nan Ke Xue, 16, 300-4.
  22. Pluth JM, Ramsey MJ, Tucker JD (2000). Role of maternal exposures and newborn genotypes on newborn chromosome aberration frequencies. Mutat Res, 465, 101-11. https://doi.org/10.1016/S1383-5718(99)00217-X
  23. Rignell-Hydbom A, Rylander L, Giwercman A, et al (2005). Exposure to PCBs and p, p’-DDE and human sperm chromatin integrity. Environ Health Perspect, 113, 175-9.
  24. Sambrook J and Russell DW (2006). Isolation of Highmolecular-weight DNA from mammalian cells using proteinase k and phenol. CSH Protoc, 1, 2006
  25. Saremi L, Imani S, Rostaminia M, et al (2014). Parental agerelated risk of retinoblastoma in Iranian children. Asian Pac J Cancer Prev, 15, 2847-50 https://doi.org/10.7314/APJCP.2014.15.6.2847
  26. Skinner MK, Guerrero-Bosagna C, Haque M, et al (2013). Environmentally induced transgenerational epigenetic reprogramming of primordial germ cells and the subsequent germ line. PLoS One, 8, 1-7.
  27. Sobus SL and Warren GW (2014). The biologic effects of cigarette smoke on cancer cells. Cancer, 120, 3617- 26. https://doi.org/10.1002/cncr.28904
  28. Tabb MM and Blumberg B (2006). New modes of action for endocrine-disrupting chemicals. Mol Endocrinol, 20, 475-82. https://doi.org/10.1210/me.2004-0513
  29. Tabrizi MM and Bidgoli SA (2015). Increased risk of childhood acute lymphoblastic leukemia (ALL) by prenatal and postnatal exposure to high voltage power lines : a case control study in Isfahan, Iran. Asian Pac J Cancer Prev, 16, 2347-50 https://doi.org/10.7314/APJCP.2015.16.6.2347
  30. Thilagavathi J, Mishra SS, Kumar M, et al (2013). Analysis of telomere length in couples experiencing idiopathic recurrent pregnancy loss. J Assist Reprod Genet, 30, 793-98. https://doi.org/10.1007/s10815-013-9993-1
  31. Toppari J, Larsen JC, Christiansen P, et al (1996). Male reproductive health and environmental xenoestrogens. Environ Health Perspect, 104, 741-803. https://doi.org/10.1289/ehp.96104s4741
  32. WHO laboratory manual (1999). Cambridge university press, cambridge.

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