Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of a c-MYC Gene Polymorphism (g.3350G>C) on Meat Quality Traits in Berkshire

  • Oh, J.D. (Department of Animal Biotechnology, Chonbuk National University) ;
  • Kim, E.S. (Department of Animal Science, Iowa State University) ;
  • Lee, H.K. (Department of Animal Biotechnology, Chonbuk National University) ;
  • Song, K.D. (Department of Animal Biotechnology, Chonbuk National University)
  • Received : 2015.05.14
  • Accepted : 2015.08.14
  • Published : 2015.11.01
Download PDF
⟨ Previous Next ⟩

Abstract

c-MYC (v-myelocytomatosis viral oncogene homologue) is a transcription factor that plays important role in many biological process including cell growth and differentiation, such as myogenesis and adipogenesis. In this study, we aimed to detect MYC gene polymorphisms, their genotype frequencies and to determine associations between these polymorphisms and meat quality traits in Berkshire pigs. We identified a single nucleotide polymorphism (SNP) in intron 2 of MYC gene by Sanger sequencing, i.e., g.3350G>C (rs321898326), that is only found in Berkshire pigs, but not in other breeds including Duroc, Landrace, and Yorkshire pigs that were used in this study. Genotypes of total 378 Berkshire pigs (138 sows and 240 boars) were determined using Hha I restriction enzyme digestion after polymerase chain reaction. Observed allele frequencies of GG, GC, and CC genotypes were 0.399, 0.508, and 0.093 respectively. Statistical analysis indicated that the g.3350G>C polymorphism was significantly associated with $pH_{45min}$ and cooking loss (p<0.05), suggesting that g.3350G>C SNP can be used for pre-selection of $pH_{45min}$ and cooking loss traits in Berkshire pigs.

Keywords

References

  1. Andersson, L., C. S. Haley, H. Ellegren, S. A. Knott, M. Johansson, K. Andersson, L. Andersson-Eklund, I. Edfors-Lilja, M. Fredholm, I. Hansson, J. Hakansson, and K. Lundstrom. 1994. Genetic mapping of quantitative trait loci for growth and fatness in pigs. Science 263:1771-1774. https://doi.org/10.1126/science.8134840
  2. Barge, M. T., G. Destefanis, G. P. Toscano, and A. Brugiapaglia. 1991. Two reading techniques of the filter paper press method for measuring meat water-holding capacity. Meat Sci. 29:183-199. https://doi.org/10.1016/0309-1740(91)90065-X
  3. Cameron, N. D. 1990. Genetic and phenotypic parameters for carcass traits, meat and eating quality traits in pigs. Livest. Prod. Sci. 26:119-135. https://doi.org/10.1016/0301-6226(90)90061-A
  4. Cepica, S., A. Stratil, M. Kopecny, P. Blazkova, J. Jr. Schroffel, R. Davoli, L. Fontanesi, G. Reiner, H. Bartenshlager, and G. Moser. 2003. Linkage and QTL mapping for Sus scrofa chromosome 4. J. Anim. Breed. Genet. 120:28-37. https://doi.org/10.1046/j.0931-2668.2003.00421.x
  5. Cho, S. H., J. H. Kim, P. N. Seong, Y. M. Cho,W. T. Chung, B. Y. Park, M. O. Chung, D. H. Kim, J. M. Lee, and C. N. Ahn. 2008. Physico-chemical meat quality properties and nutritional composition of hanwoo steer beef with 1++ quality grade. Korean J. Food Sci. An. 28:422-430. https://doi.org/10.5851/kosfa.2008.28.4.422
  6. Cliplef, R. L. and R. M. McKay. 1993. Carcass quality characteristics of swine selected for reduced backfat thickness and increased growth rate. Can. J. Anim. Sci. 73:483-494. https://doi.org/10.4141/cjas93-053
  7. Collier, J. J., T. T. Doan, M. C. Daniels, J. R. Schurr, J. K. Kolls, and D. K. Scott. 2003. c-Myc is required for the glucose-mediated induction of metabolic enzyme genes. J . Biol. Chem. 278: 6588-6595. https://doi.org/10.1074/jbc.M208011200
  8. Crawford, S. M., S. J. Moeller, H. M. Zerby, K. M. Irvin, P. S. Kuber, S. G. Velleman, and T. D. Leeds. 2010. Effects of cooked temperature on pork tenderness and relationships among muscle physiology and pork quality traits in loins from Landrace and Berkshire swine. Meat Sci. 84:607-612. https://doi.org/10.1016/j.meatsci.2009.10.019
  9. Do, C. H., B. W. Cho, and D. H. Lee. 2012. Study on the prolactin receptor 3 (PRL3) gene and the retinol-binding protein 4 (RBP4) gene as candidate genes for production traits in Berkshire pigs. Asian Australas. J. Anim. Sci. 25:183-188.
  10. de Koning, D. J., A. P. Rattink, B. Harlizius, M. A. M. Groenen, E. W. Brascamp, and J. A. M. van Arendonk. 2001. Detection and characterization of quantitative trait loci for growth and reproduction traits in pigs. Livest. Prod. Sci. 72:185-198. https://doi.org/10.1016/S0301-6226(01)00226-3
  11. Edwards, D. B., C. W. Ernst, R. J. Tempelman, G. J. M. Rosa, N. E. Raney, M. D. Hoge, and R. O. Bates. 2008. Quantitative trait loci mapping in an F2 Duroc$\times$Pietrain resource population: I. Growth traits. J. Anim. Sci. 86:241-253. https://doi.org/10.2527/jas.2006-625
  12. Freytag, S. O. and T. J. Geddes. 1992. Reciprocal regulation of adipogenesis by Myc and C/EBP alpha. Science 256:379-382. https://doi.org/10.1126/science.256.5055.379
  13. Huff-Lonergan E., T. J. Baas, M. Malek, J. C. Dekkers, K. Prusa, and M. F. Rothschild. 2002. Correlations among selected pork quality traits. J. Anim. Sci. 80:617-27. https://doi.org/10.2527/2002.803617x
  14. Jeong, D. W., Y. M. Choi, S. H. Lee, J. H. Choe, K. C. Hong, H. C. Park, and B. C. Kim. 2010. Correlations of trained panel sensory values of cooked pork with fatty acid composition, muscle fiber type, and pork quality characteristics in Berkshire pigs. Meat Sci. 86:607-615. https://doi.org/10.1016/j.meatsci.2010.04.011
  15. Jung, J. H., C. W. Kim, B. Y. Park, J. S. Choi, and H. C.Park. 2011. Genetic parameter estimates for meat quality traits in berkshire pigs. J. Anim. Sci. Technol. 53:289-296. https://doi.org/10.5187/JAST.2011.53.4.289
  16. Kang, Y. K., Y. M. Choi, S. H. Lee, J. H. Choe, K. C. Hong, and B. C. Kim. 2011. Effects of myosin heavy chain isoforms on meat quality, fatty acid composition, and sensory evaluation in Berkshire pigs. Meat Sci. 89:384-389. https://doi.org/10.1016/j.meatsci.2011.04.019
  17. Kim, C. J. and E. S. Lee. 2003. Effects of quality grade on the chemical, physical and sensory characteristics of Hanwoo (Korean native cattle) beef. Meat Sci. 63:397-405. https://doi.org/10.1016/S0309-1740(02)00099-2
  18. Kipshidze, N., P. Iversen, E. Keane, D. Stein, P. Chawla, V. Skrinska, L. R. Shankar, R. Mehran, V. Chekanov, G. Dangas, R. Komorowski, C. Haudenschild, A. Khanna, M. Leon, M. H. Keelan, and J. Mose. 2002. Complete vascular healing and sustained suppression of neointimal thickening after local delivery of advanced c-myc antisense at six months follow-up in a rabbit balloon injury model. Cardiovasc. Radiat. Med. 3:26-30. https://doi.org/10.1016/S1522-1865(02)00149-X
  19. Lee, E. A., J. M. Kim, K. S. Lim, Y. C. Ryu, W. M. Jeon, and K. C. Hong. 2012. Effects of variation in porcine MYOD1 gene on muscle fiber characteristics, lean meat production, and meat quality traits. Meat Sci. 92:36-43. https://doi.org/10.1016/j.meatsci.2012.03.018
  20. Lee, S. H., Y. M. Choi, J. H. Choe, J. M. Kim, K. C. Hong, H. C. Park, and B. C. Kim. 2010. Association between polymorphisms of the heart fatty acid binding protein gene and intramuscular fat content, fatty acid composition, and meat quality in Berkshire breed. Meat Sci. 86:794-800. https://doi.org/10.1016/j.meatsci.2010.06.024
  21. Levens, D., R. C. Duncan, T. Tomonaga, G. A. Michelotti, I. Collins, T. Davis-Smyth, T. Zheng, and E. F. Michelotti. 1997. DNA conformation, topology, and the regulation of c-myc expression. Curr. Top. Microbiol. Immunol. 224:33-46.
  22. Liu, G., D. G. Jennen, E. Tholen, H. Juengst, T. Kleinwachter, M. Holker, D. Tesfaye, G. Un, H. J. Schreinemachers, E. Murani, S. Ponsuksili, J. J. Kim, K. Schellander, and K. Wimmers. 2007. A genome scan reveals QTL for growth, fatness, leanness and meat quality in a Duroc-Pietrain resource population. Anim. Genet. 38:241-252. https://doi.org/10.1111/j.1365-2052.2007.01592.x
  23. MacDougald, O. A. and M. D. Lane. 1995. Transcriptional regulation of gene expression during adipocyte differentiation. Annu. Rev. Biochem. 64:345-373. https://doi.org/10.1146/annurev.bi.64.070195.002021
  24. Marklund, L., P. E. Nystrom, S. Stern, L. Andersson-Eklund, and L. Andersson. 1999. Confirmed quantitative trait loci for fatness and growth on pig chromosome 4. Heredity 82:134-141. https://doi.org/10.1038/sj.hdy.6884630
  25. Miner, J. H. and B. J. Wold. 1991. c-myc inhibition of MyoD and myogenin-initiated myogenic differentiation. Mol. Cell. Biol. 11:2842-2851. https://doi.org/10.1128/MCB.11.5.2842
  26. Ninomiya-Tsuji, J., F. M. Torti, and G. M. Ringold. 1993. Tumor necrosis factor-induced c-myc expression in the absence of mitogenesis is associated with inhibition of adipocyte differentiation. Proc. Natl. Acad. Sci. USA. 90:9611-9615. https://doi.org/10.1073/pnas.90.20.9611
  27. Oh, J. D., K. D. Song, J. H. Seo, D. K. Kim, S. H. Kim, K. S. Seo, H. T. Lim, J. B. Lee, H. C. Park, Y. C. Ryu, M. S. Kang, S. Cho, E. S. Kim, H. S. Choe, H. S. Kong, and H. K. Lee. 2014. Genetic traceability of black pig meats using microsatellite markers. Asian Australas. J. Anim. Sci. 27:926-931. https://doi.org/10.5713/ajas.2013.13829
  28. Park, B. Y., S. H. Cho, J. H. Kim, P. N. Seong, G. H. Kang, D. W. Jeong, C. Kim, H. C. Park, J. H. Jeong, J. S. Choi, and D. H. Kim. 2010. Comparison of pork quality by different berkshire line. Korean J. Food Sci. Ani. Resour. 30:867-871. https://doi.org/10.5851/kosfa.2010.30.5.867
  29. Potter, M. and K. B. Marcu.1997. The c-myc story: Where we've been, where we seem to be going. Curr. Top. Microbiol. Immunol. 224:1-17.
  30. Reiner, G., W. Hecht, T. Leeb, B. Brenig, A. Robic, and V. Dzapo. 1999. Isolation and characterization of the porcine c-myc proto-oncogene and chromosomal assignment to SSC 4p13. Anim. Genet. 30:204-206. https://doi.org/10.1046/j.1365-2052.1999.00447.x
  31. Schmidt, T. B., M. W. Schilling, J. M. Behrends, V. Battula, V. Jackson, R. K. Sekhon, and T. E. Lawrence. 2010. Use of cluster analysis and preference mapping to evaluate consumer acceptability of choice and select bovine M. longissimus lumborum steaks cooked to various end-point temperatures. Meat Sci. 84:46-53. https://doi.org/10.1016/j.meatsci.2009.08.016
  32. Stalder, K. J., J. Maya, L. L. Christian, S. J. Moeller, and K. J. Prus. 1998. Effects of preslaughter management on the quality of carcasses from porcine stress syndrome heterozygous market hogs. J. Anim. Sci. 76:2435-2443. https://doi.org/10.2527/1998.7692435x
  33. Whitelaw, P. F. and J. E. Hesketh. 1992. Expression of c-myc and c-fos in rat skeletal muscle. Evidence for increased levels of c-myc mRNA during hypertrophy. Biochem. J. 281( Pt 1):143-147. https://doi.org/10.1042/bj2810143

Cited by

  1. RNA-seq of muscle from pigs divergent in feed efficiency and product quality identifies differences in immune response, growth, and macronutrient and connective tissue metabolism vol.19, pp.1, 2018, https://doi.org/10.1186/s12864-018-5175-y
  2. Combining multi-OMICs information to identify key-regulator genes for pleiotropic effect on fertility and production traits in beef cattle vol.13, pp.10, 2018, https://doi.org/10.1371/journal.pone.0205295
  3. Gene regulation could be attributed to TCF3 and other key transcription factors in the muscle of pubertal heifers vol.6, pp.4, 2015, https://doi.org/10.1002/vms3.278