Biomolecules & Therapeutics

  • 제23권6호
  • /
  • Pages.582-589
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  • 2015
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  • 1976-9148(pISSN)
  • /
  • 2005-4483(eISSN)
한국응용약물학회 (The Korean Society of Applied Pharmacology)
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Lifespan Extending and Stress Resistant Properties of Vitexin from Vigna angularis in Caenorhabditis elegans

  • Lee, Eun Byeol (College of Pharmacy, Woosuk University) ;
  • Kim, Jun Hyeong (College of Pharmacy, Woosuk University) ;
  • Cha, Youn-Soo (Department of Food Science and Human Nutrition, Chonbuk National University) ;
  • Kim, Mina (Department of Food Science and Human Nutrition, Chonbuk National University) ;
  • Song, Seuk Bo (Department of Functional Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Cha, Dong Seok (College of Pharmacy, Woosuk University) ;
  • Jeon, Hoon (College of Pharmacy, Woosuk University) ;
  • Eun, Jae Soon (College of Pharmacy, Woosuk University) ;
  • Han, Sooncheon (College of Pharmacy, Woosuk University) ;
  • Kim, Dae Keun (College of Pharmacy, Woosuk University)
  • 투고 : 2015.08.13
  • 심사 : 2015.09.30
  • 발행 : 2015.11.01
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초록

Several theories emphasize that aging is closely related to oxidative stress and disease. The formation of excess ROS can lead to DNA damage and the acceleration of aging. Vigna angularis is one of the important medicinal plants in Korea. We isolated vitexin from V. angularis and elucidated the lifespan-extending effect of vitexin using the Caenorhabditis elegans model system. Vitexin showed potent lifespan extensive activity and it elevated the survival rates of nematodes against the stressful environments including heat and oxidative conditions. In addition, our results showed that vitexin was able to elevate antioxidant enzyme activities of worms and reduce intracellular ROS accumulation in a dose-dependent manner. These studies demonstrated that the increased stress tolerance of vitexin-mediated nematode could be attributed to increased expressions of stress resistance proteins such as superoxide dismutase (SOD-3) and heat shock protein (HSP-16.2). In this work, we also studied whether vitexin-mediated longevity activity was associated with aging-related factors such as progeny, food intake, growth and movement. The data revealed that these factors were not affected by vitexin treatment except movement. Vitexin treatment improved the body movement of aged nematode, suggesting vitexin affects healthspan as well as lifespan of nematode. These results suggest that vitexin might be a probable candidate which could extend the human lifespan.

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