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Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Measurement of Leukocyte Arylsulfatase A Activity Using a Natural Substrate

  • Han, Minje (Department of Laboratory Medicine, Seoul National University College of Medicine) ;
  • Jun, Sun-Hee (Department of Laboratory Medicine, Seoul National University Bundang Hospital) ;
  • Song, Sang Hoon (Department of Laboratory Medicine, Seoul National University College of Medicine) ;
  • Park, Hyung-Doo (Department of Laboratory Medicine & Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine) ;
  • Park, Kyoung Un (Department of Laboratory Medicine, Seoul National University College of Medicine) ;
  • Song, Junghan (Department of Laboratory Medicine, Seoul National University College of Medicine)
  • Received : 2014.01.21
  • Accepted : 2014.06.10
  • Published : 2015.01.02

Abstract

Keywords

Acknowledgement

Supported by : Ministry of Health and Welfare

References

  1. Baum H, Dodgson KS, Spencer B. The assay of arylsulphatases A and B in human urine. Clin Chim Acta 1959;4:453-5. https://doi.org/10.1016/0009-8981(59)90119-6
  2. Harinath BC and Robins E. Arylsulphatases in human brain: assay, some properties, and distribution. J Neurochem 1971;18:237-44. https://doi.org/10.1111/j.1471-4159.1971.tb00562.x
  3. Lee-Vaupel M and Conzelmann E. A simple chromogenic assay for arylsulfatase A. Clin Chim Acta 1987;164:171-80. https://doi.org/10.1016/0009-8981(87)90068-4
  4. Rip JW and Gordon BA. A simple spectrophotometric enzyme assay with absolute specificity for arylsulfatase A. Clin Biochem 1998;31:29-31. https://doi.org/10.1016/S0009-9120(97)00142-2
  5. Spacil Z, Tatipaka H, Barcenas M, Scott CR, Turecek F, Gelb MH. High-throughput assay of 9 lysosomal enzymes for newborn screening. Clin Chem 2013;59:502-11. https://doi.org/10.1373/clinchem.2012.189936
  6. Han M, Jun SH, Song SH, Park KU, Kim JQ, Song J. Use of tandem mass spectrometry for newborn screening of 6 lysosomal storage disorders in a Korean population. Korean J Lab Med 2011;31:250-6. https://doi.org/10.3343/kjlm.2011.31.4.250
  7. Norris AJ, Whitelegge JP, Yaghoubian A, Alattia JR, Prive GG, Toyokuni T, et al. A novel mass spectrometric assay for the cerebroside sulfate activator protein (saposin B) and arylsulfatase A. J Lipid Res 2005;46:2254-64. https://doi.org/10.1194/jlr.M500188-JLR200

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  2. Metachromatic Leukodystrophy: Diagnosis, Modeling, and Treatment Approaches vol.7, pp.None, 2020, https://doi.org/10.3389/fmed.2020.576221
  3. Leukocyte and Dried Blood Spot Arylsulfatase A Assay by Tandem Mass Spectrometry vol.92, pp.9, 2020, https://doi.org/10.1021/acs.analchem.9b05274
  4. Substrate reduction therapy for Krabbe disease and metachromatic leukodystrophy using a novel ceramide galactosyltransferase inhibitor vol.11, pp.1, 2015, https://doi.org/10.1038/s41598-021-93601-1