Annals of Laboratory Medicine

Korean Society for Laboratory Medicine (대한진단검사의학회)
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Inflammatory Cytokines and Their Prognostic Ability in Cases of Major Burn Injury

  • Hur, Jun (Department of Burn Surgery, Hallym University Hangang Sacred Heart Hospital, Hallym University College of Medicine) ;
  • Yang, Hyeong Tae (Department of Burn Surgery, Hallym University Hangang Sacred Heart Hospital, Hallym University College of Medicine) ;
  • Chun, Wook (Department of Burn Surgery, Hallym University Hangang Sacred Heart Hospital, Hallym University College of Medicine) ;
  • Kim, Jong-Hyun (Department of Burn Surgery, Hallym University Hangang Sacred Heart Hospital, Hallym University College of Medicine) ;
  • Shin, Seon-Hee (Department of Pediatrics, Hallym University Dongtan Sacred Heart Hospital, Hallym University College of Medicine) ;
  • Kang, Hee Jung (Department of Laboratory Medicine, Hallym University Sacred Heart Hospital, Hallym University College of Medicine) ;
  • Kim, Hyun Soo (Department of Laboratory Medicine, Hallym University Dongtan Sacred Heart Hospital, Hallym University College of Medicine)
  • Received : 2014.06.11
  • Accepted : 2014.11.21
  • Published : 2015.01.02
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Abstract

Background: Major burn injuries induce inflammatory responses and changes in the levels of various cytokines. This study was conducted to assess early changes in the serum levels of inflammatory cytokines after burn injury, identify cytokines associated with mortality, and characterize correlations among cytokines. Methods: Blood samples of 67 burn patients were collected on days 1 and 3 after burn injury, and the concentrations of 27 cytokines were measured using the Bio-Plex Suspension Array System (Bio-Rad Laboratories, USA). Blood samples of 25 healthy subjects were used as controls. We analyzed statistical differences in the concentrations of each cytokine between the control and patient groups, between day 1 and day 3, and between survival and nonsurvival groups. Correlations among 27 cytokines were analyzed. Results: Median concentrations of granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GM-CSF), interleukin 1 receptor antagonist (IL-1RA), interleukin 6 (IL-6), interleukin 8 (IL-8), interleukin 10 (IL-10), interleukin 15 (IL-15), monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein $1{\beta}$ ($MIP-1{\beta}$), and vascular endothelial growth factor (VEGF) were significantly higher in burn patients than in controls. IL-1RA, IL-6, and MCP-1 levels were significantly higher in the nonsurvival group than in the survival group on day 1 after burn injury. Correlation analysis of 27 cytokines showed different relationships with one another. Stronger correlations among interferon ${\gamma}$ ($IFN-{\gamma}$), IL-2, IL-4, IL-7, IL-12p70, and IL-17 were found. Conclusions: IL-1RA, IL-6, and MCP-1 may be used as prognostic indicators of mortality in burn patients and the increase in cytokine concentrations is induced by interactions within a complex network of cytokine-related pathways.

Keywords

Acknowledgement

Supported by : Hallym University Medical Center

References

  1. Finnerty CC, Herndon DN, Przkora R, Pereira CT, Oliveira HM, Queiroz DM, et al. Cytokine expression profile over time in severely burned pediatric patients. Shock 2006;26:13-9.
  2. Elias JA, Freundlich B, Kern JA, Rosenbloom J. Cytokine networks in the regulation of inflammation and fibrosis in the lung. Chest 1990;97:1439-45. https://doi.org/10.1378/chest.97.6.1439
  3. Ozaki K and Leonard WJ. Cytokine and cytokine receptor pleiotropy and redundancy. J Biol Chem 2002;277:29355-8. https://doi.org/10.1074/jbc.R200003200
  4. Mikhal’chik EV, Piterskaya JA, Budkevich LY, Pen’kov LY, Facchiano A, De Luca C, et al. Comparative study of cytokine content in the plasma and wound exudate from children with severe burns. Bull Exp Biol Med 2009;148:771-5. https://doi.org/10.1007/s10517-010-0813-7
  5. Jeschke MG, Gauglitz GG, Kulp GA, Finnerty CC, Williams FN, Kraft R, et al. Long-term persistence of the pathophysiologic response to severe burn injury. PLoS ONE 2011;6:e21245. https://doi.org/10.1371/journal.pone.0021245
  6. Davis CS, Janus SE, Mosier MJ, Carter SR, Gibbs JT, Ramirez L, et al. Inhalation injury severity and systemic immune perturbations in burned adults. Ann Surg 2013;257:1137-46. https://doi.org/10.1097/SLA.0b013e318275f424
  7. Gauglitz GG, Finnerty CC, Herndon DN, Mlcak RP, Jeschke MG. Are serum cytokines early predictors for the outcome of burn patients with inhalation injuries who do not survive? Crit Care 2008;12:R81. https://doi.org/10.1186/cc6932
  8. Csontos C, Foldi V, Palenkas L, Bogar L, Roth E, Weber G, et al. Time course of pro- and anti-inflammatory cytokine levels in patients with burns-prognostic value of interleukin-10. Burns 2010;36:483-94. https://doi.org/10.1016/j.burns.2009.10.009
  9. Kim HS, Kim JH, Yim H, Kim D. Changes in the levels of interleukins 6, 8, and 10, tumor necrosis factor alpha, and granulocyte-colony stimulating factor in Korean burn patients: relation to burn size and postburntime. Ann Lab Med 2012;32:339-44. https://doi.org/10.3343/alm.2012.32.5.339
  10. Finnerty CC, Jeschke MG, Herndon DN, Gamelli R, Gibran N, Klein M, et al. Temporal cytokine profiles in severely burned patients: a comparison of adults and children. Mol Med 2008;14:553-60.
  11. Lantos J, Foldi V, Roth E, Weber G, Bogar L, Csontos C. Burn trauma induces early HMGB1 release in patients: its correlation with cytokines. Shock 2010;33:562-7. https://doi.org/10.1097/SHK.0b013e3181cd8c88

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