The Korean journal of internal medicine

  • 제30권1호
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  • Pages.62-72
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  • 2015
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  • 1226-3303(pISSN)
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  • 2005-6648(eISSN)
대한내과학회 (The Korean Association of Internal Medicine)
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DOI QR Code

Proteomic differences with and without ozone-exposure in a smoking-induced emphysema lung model

  • Uh, Soo-Taek (Division of Allergy and Respiratory Medicine, Department of Internal Medicine, Soonchunhyang University Seoul Hospital) ;
  • Koo, So-My (Division of Allergy and Respiratory Medicine, Department of Internal Medicine, Soonchunhyang University Seoul Hospital) ;
  • Jang, An Soo (Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital) ;
  • Park, Sung Woo (Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital) ;
  • Choi, Jae Sung (Division of Respiratory Medicine, Department of Internal Medicine, Soonchunhyang University Cheonan Hospital) ;
  • Kim, Yong-Hoon (Division of Respiratory Medicine, Department of Internal Medicine, Soonchunhyang University Cheonan Hospital) ;
  • Park, Choon Sik (Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital)
  • 투고 : 2014.05.13
  • 심사 : 2014.09.25
  • 발행 : 2015.01.01
⟨ 이전 논문 다음 논문 ⟩

초록

Background/Aims: Acute exacerbations in chronic obstructive pulmonary disease may be related to air pollution, of which ozone is an important constituent. In this study, we investigated the protein profiles associated with ozone-induced exacerbations in a smoking-induced emphysema model. Methods: Mice were divided into the following groups: group I, no smoking and no ozone (NS + NO); group II, no smoking and ozone (NS + O); group III, smoking and no ozone (S + NO); and group IV, smoking and ozone (S + O). Bronchoalveolar lavage, the mean linear intercept (MLI) on hematoxylin and eosin staining, nano-liquid chromatography-tandem mass spectrometry (LC-MS/MS), and Western blotting analyses were performed. Results: The MLIs of groups III (S + NO) and IV (S + O) ($45{\pm}2$ and $44{\pm}3{\mu}m$, respectively) were significantly higher than those of groups I (NS + NO) and II (NS + O) ($26{\pm}2$ and$23{\pm}2{\mu}m$, respectively; p < 0.05). Fourteen spots that showed significantly different intensities on image analyses of two-dimensional (2D) protein electrophoresis in group I (NS + NO) were identified by LC-MS/MS. The levels of six proteins were higher in group IV (S + O). The levels of vimentin, lactate dehydrogenase A, and triose phosphate isomerase were decreased by both smoking and ozone treatment in Western blotting and proteomic analyses. In contrast, TBC1 domain family 5 (TBC1D5) and lamin A were increased by both smoking and ozone treatment. Conclusions: TBC1D5 could be a biomarker of ozone-induced lung injury in emphysema.

키워드

과제정보

연구 과제 주관 기관 : Ministry of Health

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