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First Report and Characterization of Pestalotiopsis ellipsospora Causing Canker on Acanthopanax divaricatus

  • Received : 2015.07.01
  • Accepted : 2015.09.06
  • Published : 2015.09.30

Abstract

Acanthopanax divaricatus, a member of the Araliaceae family, has been used as an invigorant in traditional Korean medicine. During disease monitoring, a stem with small, irregular, brown lesions was sampled at a farm in Cheonan in 2011. The symptoms seen were sunken cankers and reddish-brown needles on the infected twig. The isolated fungal colonies were whitish, having crenated edges and aerial mycelium on the surface, and with black gregarious fruiting bodies. The reverse plate was creamy white. Conidia were $17{\sim}22{\times}3.5{\sim}4.2{\mu}m$, fusiform, 4-septate, and straight to slightly curved. The nucleotide sequence of the partial translation elongation factor 1 alpha gene of the fungal isolate, shares 99% sequence identity with that of known Pestalotiopsis ellipsospora. Based on the results of the morphological and molecular analyses, the fungal isolate was identified as P. ellipsospora. In Korea, this is the first report of canker on A. divaricatus.

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References

  1. Ohwi J. Flora of Japan. Tokyo: Shibundo; 1972.
  2. Li XY. Immunomodulating Chinese herbal medicines. Mem Inst Oswaldo Cruz 1991;86 Suppl 2:159-64. https://doi.org/10.1590/S0074-02761991000600036
  3. Jung BS, Shin MK. Hyang Yak Dae Sa Jeon. 3rd ed. Seoul: Young Lim Sa Publisher; 2003.
  4. Hong SS, Hwang JS, Lee SA, Hwang BY, Ha KW, Ze KR, Seung RS, Ro JS, Lee KS. Isolation and quantitative analysis of acanthoside D from Acanthopanacis cortex. Korean J Pharmacogn 2001;32:316-21.
  5. Lee S, Son D, Ryu J, Lee YS, Jung SH, Kang J, Lee SY, Kim HS, Shin KH. Anti-oxidant activities of Acanthopanax senticosus stems and their lignan components. Arch Pham Res 2004;27:106-10. https://doi.org/10.1007/BF02980055
  6. Nhiem NX, Kiem PV, Minh CV, Tai BH, Quang TH, Soung KS, Koo JE, Koh YS, Kim YH. Anti-inflammatory activity on LPS-stimulated dendritic cells of lupane-type triterpenoids from the leaves of Acanthopanax koreanum. Arch Pharm Res 2011;34:1593-8. https://doi.org/10.1007/s12272-011-1002-6
  7. Bate-Smith EC, Metcalfe CR. Leuco-anthocyanins. 3. The nature and systematic distribution of tannins in dicotyledonous plants. J Linn Soc Lond Bot 1957;55:669-705. https://doi.org/10.1111/j.1095-8339.1957.tb00030.x
  8. Yasuda F, Kobayashi T, Watanabe H, Izawa H. Addition of Pestalotiopsis spp. to leaf spot pathogens of Japanese persimmon. J Gen Plant Pathol 2003;69:29-32. https://doi.org/10.1007/s10327-002-0011-1
  9. Das R, Chutia M, Das K, Jha DK. Factors affecting sporulation of Pestalotiopsis disseminata causing grey blight disease of Persea bombycina Kost., the primary food plant of muga silkworm. Crop Prot 2010;29:963-8. https://doi.org/10.1016/j.cropro.2010.05.012
  10. Maharachchikumbura SS, Guo LD, Chukeatirote E, Bahkali AH, Hyde KD. Pestalotiopsis: morphology, phylogeny, biochemistry and diversity. Fungal Divers 2011;50:167-87. https://doi.org/10.1007/s13225-011-0125-x
  11. Korean Society of Plant Pathology. List of plant disease in Korea. 5th ed. Seoul: Korean Society of Plant Pathology; 2009.
  12. Maharachchikumbura SS, Guo LD, Cai L, Chukeatirote E, Wu WP, Sun X, Crous PW, Bhat DJ, McKenzie EH, Bahkali AH, et al. A multi-locus backbone tree for Pestalotiopsis, with a polyphasic characterization of 14 new species. Fungal Divers 2012;56:95-129. https://doi.org/10.1007/s13225-012-0198-1
  13. Korea Crop Protection Association. Agrochemical use guide book. Seoul: Korea Crop Protection Association; 2007.
  14. Kim SH, Uzunovic A, Breuil C. Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR. Appl Environ Microbiol 1999;65:287-90.
  15. Carbone I, Kohn LM. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 1999;91:553-6. https://doi.org/10.2307/3761358
  16. Samuels GJ, Dodd SL, Gams W, Castlebury LA, Petrini O. Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus. Mycologia 2002;94:146-70. https://doi.org/10.2307/3761854
  17. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-9. https://doi.org/10.1093/molbev/msr121