Mycobiology

The Korean Society of Mycology (한국균학회)
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Methyl 9-Oxo-(10E,12E)-octadecadienoate Isolated from Fomes fomentarius Attenuates Lipopolysaccharide-Induced Inflammatory Response by Blocking Phosphorylation of STAT3 in Murine Macrophages

  • Choe, Ji-Hyun (Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental & Bioresources, Chonbuk National University) ;
  • Yi, Young-Joo (Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental & Bioresources, Chonbuk National University) ;
  • Lee, Myeong-Seok (Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental & Bioresources, Chonbuk National University) ;
  • Seo, Dong-Won (Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental & Bioresources, Chonbuk National University) ;
  • Yun, Bong-Sik (Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental & Bioresources, Chonbuk National University) ;
  • Lee, Sang-Myeong (Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental & Bioresources, Chonbuk National University)
  • Received : 2015.08.13
  • Accepted : 2015.09.08
  • Published : 2015.09.30
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Abstract

Fomes fomentarius is a fungus of the Polyporaceae family and is used in traditional oriental therapies. Although the anti-inflammatory activities of this species have been previously reported, the identity of the bioactive compounds responsible for this activity remains unknown. Here, we investigated whether methyl 9-oxo-(10E,12E)-octadecadienoate (FF-8) purified from F. fomentarius exerts anti-inflammatory activity in murine macrophages stimulated with lipopolysaccharide (LPS). FF-8 suppressed secretion of nitric oxide (NO) and prostaglandin $E_2$ through downregulation of inducible NO synthase and cyclooxygenase-2 expression induced by LPS. In addition, pretreatment of cells with FF-8 led to a reduction in levels of secreted inflammatory cytokines such as tumor necrosis factor-${\alpha}$ and interleukin-6 in macrophages stimulated with LPS. Conversely, FF-8 did not affect nuclear factor ${\kappa}B$, p38, c-Jun NH2-terminal kinase, and extracellular signal-regulated kinase pathways. Instead, FF-8 specifically interfered with signal transducer and activator of transcription 3 (STAT3) phosphorylation induced by LPS. Collectively, this study demonstrated that FF-8 purified from F. fomentarius suppresses inflammatory responses in macrophages stimulated with LPS by inhibiting STAT3 activation. Further studies will be required to elucidate the anti-inflammatory effect of FF-8 in vivo.

Keywords

References

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