대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)

  • 제29권3호
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  • Pages.54-64
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  • 2015
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  • 1226-8038(pISSN)
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  • 2287-9463(eISSN)
대한한방소아과학회 (The Association of Korean Oriental Pediatrics)
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금은화 추출물의 항산화, 항염증 효과가 Dextran Sulfate Sodium으로 유도된 생쥐의 궤양성 대장염에 미치는 영향

The Anti-oxidative and Anti-inflammatory Effect of Lonicera Japonica on Ulcerative Colitis Induced by Dextran Sulfate Sodium in Mice

  • 차호열 (부산대학교한방병원 한방소아과) ;
  • 정아람 (부산대학교한방병원 한방소아과) ;
  • 천진홍 (부산대학교한방병원 한방소아과) ;
  • 안상현 (동국대학교 한의과대학 해부학교실) ;
  • 박선영 (세명대학교 한의과대학 생리학교실) ;
  • 김기봉 (부산대학교한방병원 한방소아과)
  • Cha, Ho Yeol (Department of Pediatrics of Korean Medicine, Pusan National University Hospital) ;
  • Jeong, A Ram (Department of Pediatrics of Korean Medicine, Pusan National University Hospital) ;
  • Cheon, Jin Hong (Department of Pediatrics of Korean Medicine, Pusan National University Hospital) ;
  • Ahn, Sang Hyun (Department of Anatomy, College of Korean Medicine, Dongguk University) ;
  • Park, Sun Young (Department of Physiology, College of Korean Medicine, Semyung University) ;
  • Kim, Ki Bong (Department of Pediatrics of Korean Medicine, Pusan National University Hospital)
  • 투고 : 2015.07.30
  • 심사 : 2015.08.14
  • 발행 : 2015.08.31
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초록

Objectives : This study was to investigate the anti-oxidative and anti-inflammatory effect of Lonicera japonica water extracts (LE) on Ulcerative Colitis Induced by DSS (Dextran Sulfate Sodium) in Mice. Methods : Colitis was induced by DSS in Balb/c mice. The sample group was divided into three. The mice in control group were not inflammation-induced. The pathological group was composed of untreated colitis elicited mice. The experimental group was administered Lonicera japonica water extracts (LE) after colitis elicitation. The effects on ulcerative colitis were evaluated the anti-oxidant effect, inhibition of COX-2 mRNA expression, the morphological change of colonic mucosa, decrease effect of HSP 70 and COX-2 in mucosa. Results : The SOD ability of LE was dose-dependently increased and the LPS-induced COX-2 mRNA expression of LE was dose-dependently decreased. LE showed the protective effects on DSS-induced experimental colitis. LE inhibited shortening of colon length, the hemorrhagic erosion in colonic mucosa. LE also showed the decrease effect for HSP70 and COX-2 in mucosa. Conclusions : The current results demonstrate the clinical utility of LE in traditional medicine and indicate the possible treatments for ulcerative colitis from natural products. Further investigations for exact mechanisms will be needed.

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