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An Efficient PEG/CaCl2-Mediated Transformation Approach for the Medicinal Fungus Wolfiporia cocos

  • Sun, Qiao (College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University) ;
  • Wei, Wei (Institute for Interdisciplinary Research, Jianghan University) ;
  • Zhao, Juan (College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University) ;
  • Song, Jia (College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University) ;
  • Peng, Fang (College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University) ;
  • Zhang, Shaopeng (College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University) ;
  • Zheng, Yonglian (College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University) ;
  • Chen, Ping (College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University) ;
  • Zhu, Wenjun (College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University)
  • Received : 2015.01.20
  • Accepted : 2015.05.19
  • Published : 2015.09.28

Abstract

Sclerotia of Wolfiporia cocos are of medicinal and culinary value. The genes and molecular mechanisms involved in W. cocos sclerotial formation are poorly investigated because of the lack of a suitable and reproducible transformation system for W. cocos. In this study, a PEG/CaCl2-mediated genetic transformation system for W. cocos was developed. The promoter Pgpd from Ganoderma lucidum effectively drove expression of the hygromycin B phosphotransferase gene in W. cocos, and approximately 30 transformants were obtained per 10 μg DNA when the protoplast suspension density was 106 protoplasts/ml. However, no transformants were obtained under the regulation of the PtrpC promoter from Aspergillus nidulans.

Keywords

References

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