Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A Phenylpropanoid Glycoside as a Calcineurin Inhibitor Isolated from Magnolia obovata Thunb.

  • Lee, Won Jeong (Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology) ;
  • Moon, Jae Sun (Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Sung In (Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology) ;
  • Bahn, Yong-Sun (Department of Biotechnology, Yonsei University) ;
  • Lee, Hanna (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Kang, Tae Hoon (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Shin, Heung Mook (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Kim, Sung Uk (Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology)
  • Received : 2015.06.12
  • Accepted : 2015.06.24
  • Published : 2015.09.28
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Abstract

To identify plant-derived cell signaling inhibitors with antifungal properties, a twocomponent screening system using both wild-type Cryptococcus neoformans and a calcineurin mutant was employed owing to their counter-regulatory actions on the Hog1 mitogenactivated protein kinase and calcineurin pathways. Of the 2,000 plant extracts evaluated, a single bioactive compound from M. obovata Thunb. was found to act specifically on the calcineurin pathway of C. neoformans. This compound was identified as magnoloside A, and had potent antifungal activities against various Cryptococcus strains with minimum inhibitory concentration values ranging from 1.0 to 4.0 μg/ml.

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References

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