Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Visual detection of porcine circovirus 2 by loop-mediated isothermal amplification (LAMP) with hydroxynaphthol blue dye

육안 판독 등온증폭법을 이용한 돼지 써코바이러스 2형 신속 진단법

  • Kong, Ho-Chul (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Kim, Eun-Mi (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Jeon, Hyo-Sung (M monitor Incorporation) ;
  • Kim, Ji-Jung (M monitor Incorporation) ;
  • Kim, Hee-Jung (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Park, Yu-Ri (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Kang, Dae-Young (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Kim, Young-Hwa (National Institute of Animal Science, RDA) ;
  • Park, Jun-Cheol (National Institute of Animal Science, RDA) ;
  • Lee, Chang-hee (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Yeo, Sang-Geon (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Park, Choi-Kyu (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University)
  • 공호철 (경북대학교 수의과대학 & 수의전염병제어센터) ;
  • 김은미 (경북대학교 수의과대학 & 수의전염병제어센터) ;
  • 전효성 (엠모니터) ;
  • 김지정 (엠모니터) ;
  • 김희정 (경북대학교 수의과대학 & 수의전염병제어센터) ;
  • 박유리 (경북대학교 수의과대학 & 수의전염병제어센터) ;
  • 강대영 (경북대학교 수의과대학 & 수의전염병제어센터) ;
  • 김영화 (국립축산과학원) ;
  • 박준철 (국립축산과학원) ;
  • 이창희 (경북대학교 자연과학대학) ;
  • 여상건 (경북대학교 수의과대학 & 수의전염병제어센터) ;
  • 박최규 (경북대학교 수의과대학 & 수의전염병제어센터)
  • Received : 2015.08.05
  • Accepted : 2015.09.02
  • Published : 2015.09.30
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Abstract

In this study, we developed a loop-mediated isothermal amplification (LAMP) with hydroxynaphtol blue dye (HNB) for rapid and direct visual detection of porcine circovirus 2 DNA with high sensitivity and specificity. The LAMP was completed in 40 min at $63^{\circ}C$, and the results of the LAMP can be confirmed by naked eye without any detection process. The sensitivity of the LAMP was 10-fold higher than that of the commercial PCR (cPCR) and real time PCR (rPCR) previously reported. In clinical application, the PCV2 detection rate of the LAMP was the same on porcine tissue samples (75.0%, 36/48) between porcine blood samples (75.0%, 39/52). The PCV2 detection rate (75.0%) of LAMP was higher than those of the cPCR and rPCR (67.3%, 35/52) in blood samples. In conclusion, the LAMP developed in the study could be an useful alternative method for the detection of PCV2 in the swine disease diagnostic laboratories.

Keywords

References

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