Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Identification of Copper and Cadmium Induced Genes in Alfalfa Leaves through Annealing Control Primer Based Approach

  • Lee, Ki-Won (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Rahman, Md. Atikur (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Zada, Muhammad (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Dong-Gi (Division of Life Sciences, Korea Basic Science Institute) ;
  • Kim, Ki-Yong (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Hwang, Tae Young (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Ji, Hee Jung (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Sang-Hoon (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
  • Received : 2015.09.05
  • Accepted : 2015.09.14
  • Published : 2015.10.01
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Abstract

The present research investigated copper and cadmium stress-induced differentially expressed genes (DEGs) using annealing control primers (ACP) with the differential display reverse transcription polymerase chain reaction technique in alfalfa (Medicago sativa L. cv. Vernal) leaves. Alfalfa leaves were subjected to $250{\mu}M$ of copper and cadmium treatment for a period of 6 h. A total of 120 ACPs was used. During copper and cadmium treatment, 6 DEGs were found to be up or down regulated. During copper stress treatment, 1 DEG was up-regulated, and 3 novel genes were discovered. Similarly, during cadmium stress treatment, 1 DEG was up-regulated and 5 novel genes were identified. Among all 6 DEGs, DEG-4 was identified as the gene for trans-2,3-enoyl-CoA reductase, DEG-5 was identified as the gene for senescence-associated protein DIN1 and DEG-6 was identified for caffeic acid O-methyltransferase. All the up-regulated genes may play a role in copper and cadmium stress tolerance in alfalfa.

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References

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