DOI QR코드

DOI QR Code

Prediction of Chemical Composition and Fermentation Parameters in Forage Sorghum and Sudangrass Silage using Near Infrared Spectroscopy

  • Park, Hyung-Soo (Grassland & Forages Division, National Institute of Animal Science) ;
  • Lee, Sang-Hoon (Grassland & Forages Division, National Institute of Animal Science) ;
  • Choi, Ki-Choon (Grassland & Forages Division, National Institute of Animal Science) ;
  • Kim, Ji-Hye (Grassland & Forages Division, National Institute of Animal Science) ;
  • So, Min-Jeong (Grassland & Forages Division, National Institute of Animal Science) ;
  • Kim, Hyeon-Seop (Grassland & Forages Division, National Institute of Animal Science)
  • 투고 : 2015.09.05
  • 심사 : 2015.09.07
  • 발행 : 2015.10.01

초록

This study was conducted to assess the potential of using NIRS to accurately determine the chemical composition and fermentation parameters in fresh coarse sorghum and sudangrass silage. Near Infrared Spectroscopy (NIRS) has been increasingly used as a rapid and accurate method to analyze the quality of cereals and dried animal forage. However, silage analysis by NIRS has a limitation in analyzing dried and ground samples in farm-scale applications because the fermentative products are lost during the drying process. Fresh coarse silage samples were scanned at 1 nm intervals over the wavelength range of 680~2500 nm, and the optical data were obtained as log 1/Reflectance (log 1/R). The spectral data were regressed, using partial least squares (PLS) multivariate analysis in conjunction with first and second order derivatization, with a scatter correction procedure (standard normal variate and detrend (SNV&D)) to reduce the effect of extraneous noise. The optimum calibrations were selected on the basis of minimizing the standard error of cross validation (SECV). The results of this study showed that NIRS predicted the chemical constituents with a high degree of accuracy (i.e. the correlation coefficient of cross validation ($R^2{_{cv}}$) ranged from 0.86~0.96), except for crude ash which had an $R^2{_{cv}}$ of 0.68. Comparison of the mathematical treatments for raw spectra showed that the second-order derivatization procedure produced the best result for all the treatments, except for neutral detergent fiber (NDF). The best mathematical treatment for moisture, acid detergent fiber (ADF), crude protein (CP) and pH was 2,16,16 respectively while the best mathematical treatment for crude ash, lactic acid and total acid was 2,8,8 respectively. The calibrations of fermentation products produced poorer calibrations (RPD < 2.5) with acetic and butyric acid. The pH, lactic acid and total acids were predicted with considerable accuracy at $R^2{_{cv}}$ 0.72~0.77. This study indicated that NIRS calibrations based on fresh coarse sorghum and sudangrass silage spectra have the capability of assessing the forage quality control

키워드

참고문헌

  1. AOAC, 1990. Association of Official Analytical Chemists, Official Methods of Analysis. 15th Edition. Washington, DC.
  2. Barber, D.D., Givens, D.I., Kridis, M.S., Offer N.W. and Murray I., 1990. Prediction of the organic matter digestibility of grass silage. Animal Feed Science and Technology. 28:115-128. https://doi.org/10.1016/0377-8401(90)90074-I
  3. Cozzolino, D., Fassio, A. and Gimenez, A. 2000. The use of near-infrared reflectance spectroscopy to predict the composition of whole maize plants. Journal of Science Food Agricultural. 81:142-146.
  4. Heise, H.M. and Winzen, R. 2002. Fundamental chemometric methods. In: Siesler, H.W., Ozaki, Y., Kawata, S., Heise, H.M. (Eds.), Near-Infrared Spectroscopy: Principles, Instruments, Applications. Wiley-VCH, Weinheim, pp. 125-162.
  5. Liu, X., Han, L. Yang, Z. and Xu, Ch. 2008. Prediction of silage digestibility by near infrared refelectance spectroscopy. Journal of Animal and Feed Science. 17:631-639. https://doi.org/10.22358/jafs/66691/2008
  6. Martens, H. and Martens, M. 2001. Multivariate Analysis of Quality: An Introduction. John Wiley & Sons Ltd, Chichester, UK.
  7. MAFRA. 2014. The complementary measure for increased production of forage. pp. 10-11.
  8. Park, R.S., Agnew, R.E., Gordon, F.J. and Steen, R.W.J. 1998. The use of near infrared reflectance spectroscopy on undried samples of grass silage to predict chemical composition and digestibility parameters. Animal Feed Science and Technology. 72:155-167. https://doi.org/10.1016/S0377-8401(97)00175-2
  9. Porter, M.G., 1992. Comparison of five methods for the determination of lactic acid in silage. Br. Grass. Soc., 3rd Res. Conf., pp.123-124.
  10. Reddersen, B., Fricke, T. and Michae, W.l. 2014. Effects of sample preparation and measurement standardization on the NIRS calibration quality of nitrogen, ash and NDFom content in extensive experimental grassland biomass. Animal Feed Science and Technology. 183:77-85.
  11. Reeves, III J.B. and Blosser, T.H. 1989. Near infrared reflectance spectroscopy for analyzing undried silages. Journal of Dairy Science. 72:79-88. https://doi.org/10.3168/jds.S0022-0302(89)79082-2
  12. Richter, F., Fricke, T. and Wachendorf, M. 2010. Utilization of semi-natural grassland through integrated generation of solid fuel and biogas from biomass, III. Effects of hydrothermal conditioning and mechanical dehydration on solid fuel properties and on energy and greenhouse gas balances. Grassland and Forage Science. 65:185-199. https://doi.org/10.1111/j.1365-2494.2010.00737.x
  13. Roggo, Y., Chalus, P., Maurer, L., Lema-Martinez, C., Edmond, A., and Jent, N. 2007. A review of near infrared spectroscopy and chemometrics in pharmaceutical technologies. Journal of Pharmaceutical and Biomedical Analysis. 44(3):683-700. https://doi.org/10.1016/j.jpba.2007.03.023
  14. Shenk, J.S. and Westerhaus, M.O. 1994. The application of near infrared reflectance spectroscopy (NIRS) to forage analysis. In Forage Quality Evaluation and Utilization. pp. 406. Am. Soc. Agron., Madison, WI.
  15. Siesler, H.W., Ozaki, Y., Kawata, S. and Heise, H.M. 2002. Eds. Near-Infrared Spectroscopy: Principles, Instruments, Applications; Wiley-VCH: New York.
  16. Sinnaeve, G., Dardenne, P., Agneessens, R. and Biston, R. 1994. The use of near infrared spectroscopy for the analysis of fresh grass silage. Journal of Near infrared Spectroscopy. 2:79-84. https://doi.org/10.1255/jnirs.34
  17. Snyman, L.D. and Joubert, H.W. 1992. Near-infrared reflectance analysis of the fermentation characteristics of silage prepared by chemical treatment to prevent volatilization of fermentation end products. Animal Feed Science and Technology. 37:47-58. https://doi.org/10.1016/0377-8401(92)90119-Q
  18. Stuth, J., Jama, A. and Tolleson, D. 2003. Direct and indirect means of predicting forage quality through near infrared reflectance spectroscopy. Field Crops Research. 84:45-56. https://doi.org/10.1016/S0378-4290(03)00140-0
  19. Sweeney, R.A. 1988. Generic combustion method for determination of crude protein in feeds: collaborative study. Journal-Association of Official Analytical Chemists. 72(5):770-774.
  20. Williams, P.C. 2001. Implementation of near-infrared technology. In: Near-infrared Technology in the Agricultural and Food Industries, 2nd ed. Eds Williams, P. & Norris, K., St. Paul, USA: American Association of Cereal Chemists. pp. 145-169.

피인용 문헌

  1. Mathematical Transformation Influencing Accuracy of Near Infrared Spectroscopy (NIRS) Calibrations for the Prediction of Chemical Composition and Fermentation Parameters in Corn Silage vol.36, pp.1, 2016, https://doi.org/10.5333/KGFS.2016.36.1.50