보존과학회지 (Journal of Conservation Science)

  • 제31권3호
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  • Pages.227-241
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  • 2015
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  • 1225-5459(pISSN)
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  • 2287-9781(eISSN)
한국문화재보존과학회 (The Korean Society Of Conservation Science For Cultural Heritage)
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공주 송산리 6호분에서 분리한 진균의 생화학적 특성 분석 및 생장제어 연구

Biochemical Characteristics and Growth Control for fungi isolated from mural painting of Tomb No.6 at Songsan-ri, Gongju

  • 이민영 (한국전통문화대학교 문화재예방보존연구소) ;
  • 박희문 (충남대학교 생명시스템과학대학 미생물분자생명과학과) ;
  • 정용재 (한국전통문화대학교 문화유산융합대학원 수리복원학과)
  • Lee, Min Young (Institute of Preventive Conservation for Cultural Property, Korea National University of Cultural Heritage) ;
  • Park, Hee Moon (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Graduate School, Chungnam National University) ;
  • Chung, Yong Jae (Department of Heritage Conservation & Restoration, Graduate School of Convergence Cultural Heritage, Korea National University of Cultural Heritage)
  • 투고 : 2015.07.21
  • 심사 : 2015.08.26
  • 발행 : 2015.09.20
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초록

공주 송산리 6호분에서 분리한 미생물의 배양조건에 따른 생화학적 특성을 확인하고 천연물 유효성분인 anethole과 eugenol의 항진균력을 평가하여 고분 또는 벽화에 대한 적용 가능성을 확인하였다. 송산리 6호분 내부 공기중 및 벽체에서 포집된 26종의 곰팡이는 자낭균류 15종, 접합균류 2종, 담자균류 1종으로 동정되었다. 분리된 곰팡이의 전분과 젤라틴에 대한 분해도 평가를 실시한 결과 SY-18, SY-23, SY-25 등에서 전분에 대한 분해능이 높게 나타났으며, SY-18, SY-21, SY-23 등에서 높은 젤라틴 분해능을 보였다. 각 기질의 분해능이 우수한 균주 3종을 액체배양하여 생화학적 특성을 확인하였다. 천연물 유효성분인 anethole과 eugenol의 항진균력 평가 결과, anethole과 eugenol의 혼합액(1:2)에서 곰팡이의 생장 억제력이 가장 크게 나타났다. 국내외에 동굴 또는 고분 벽화에 발생 가능한 곰팡이에 대해 강력한 항진균력을 보이는 천연물 유효성분을 이용하여 미생물의 발생을 억제할 수 있을 것으로 기대된다.

Fungi were isolated from mural painting in tomb no.6 at songsan-ri, Gong-ju. Antifungal susceptibility of essential oils extracted from natural medicine was tested and it confirmed applicability for mural painting in tombs. 26 species of fungi collected from air-borne and wall surfaces were identified to 15 species of Ascomycetes, 2 species of Zygomycetes, 1 of Basidiomycetes. Wheat starch and gelatin degradability were evaluated as isolated fungi. SY-18, SY-23, SY-25 showed high degradability of wheat starch. SY-18, SY-21, SY-23 were decomposed into gelatin. Biochemical characteristics of decomposing fungi to wheat starch glue and cowhide glue were analyzed by using ${\alpha}-amylase$ and gelatinase activity. An Antifungal test was conducted in Anethole and Eugenol. Anethole and Eugenol mixture(1:2) showed high antifungal susceptibility. Natural adhesives help microbial growth and can cause structural damage in mural painting. The expectation of this study is the possibility to control microbial growth in wall painting using natural essential oils. It can be used as a data for conservation method to control microbial damages.

키워드

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