DOI QR코드

DOI QR Code

T-786C, G894T, and Intron 4 VNTR (4a/b) Polymorphisms of the Endothelial Nitric Oxide Synthase Gene in Bladder Cancer Cases

  • Polat, Fikriye (Department of Primary Education, Elementary Sciences Education, Faculty of Education, Kocaeli University) ;
  • Diler, Songul Budak (Department of Biology, Faculty of Science and Letters, University of Nigde) ;
  • Azazi, Irfan (Luleburgaz State Hospital) ;
  • Oden, Artun (Nigde State Hospital, Nigde, Department of Urology Clinic)
  • 발행 : 2015.04.03

초록

The aim of the present study was to determine whether endothelial nitric oxide synthase (eNOS) gene polymorphisms play a role in development of bladder cancer in the Turkish population. The study was performed on 75 patients (64 men, 11 women) with bladder cancer and 143 healthy individuals (107 men, 36 women) with any kind of cancer history. Three eNOS gene polymorphisms (T-786C promoter region, G894T and intron 4 VNTR 4a/b) were determined with polymerase chain reaction and restriction fragment lenght polymorphism methods. In our study, GT and TT genotypes for eNOS G894T polymorphism were found to significantly vary among patients with bladder cancer and control group (OR: 0.185, CI: 0.078-0.439, p=0.0001 and OR: 0.324, CI: 0.106-0.990, p=0.026). Also, the frequency of the 894T allele was significantly higher in patients with bladder cancer (51%). No association was identified for eNOS T-786C and intron 4 VNTR 4a/b polymorphisms between patients with bladder cancer and control groups in our Turkish population.

키워드

참고문헌

  1. Akar N, Akar E, Cin S, et al (1999). Endothelial nitric oxide synthase intron 4, 27 bp repeat polymorphism in Turkish patients with deep vein thrombosis and cerebrovascular accidents. Thromb Res, 94, 63-4. https://doi.org/10.1016/S0049-3848(98)00193-5
  2. Akca H, Tokgun O, (2012). Genetic alteration in bladder carcinoma. Uroonkoloji Bulteni, 11, 10-3.
  3. Amasyali AK, Kucukgergin C, Erdem S, et al (2012). Nitric oxide synthase (eNOS4a/b) gene polymorphism is associated with tumor recurrence and progression in superficial bladder cancer cases. J Urology, 188, 2398-403. https://doi.org/10.1016/j.juro.2012.07.096
  4. Buyru N, Tigli H, Ozcan F, Dalay N, (2003). Ras oncogene mutations in urine sediments of patients with bladder cancer. J Biochem Mol Biol, 36, 399-402. https://doi.org/10.5483/BMBRep.2003.36.4.399
  5. Colombel, M, Picard A, (2008). Prevention of bacillus calmette-guerin immunotherapy complications. Prog Urol, 18, 105-10. https://doi.org/10.1016/S1166-7087(08)72486-2
  6. Crawford JM, (2008). The origins of bladder cancer. Laboratory Investigation, 88, 686-93. https://doi.org/10.1038/labinvest.2008.48
  7. Marcus PM, Hayes RB, Vineis P, et al (2000). Cigarette smoking, Nacetyltransferase 2 acetylation status, and bladder cancer risk: a caseseries meta-analysis of a gene-environment interaction. Cancer Epidemiol Biomarkers Prev, 9, 461-7.
  8. Glueck CJ, Munjal J, Khan A, Umar M, Wang P, (2010). Endothelial nitric oxide synthase T-786C mutation, a reversible etiology of Prinzmetal's angina oectoris. Am J Cardiol, 105, 792-6. https://doi.org/10.1016/j.amjcard.2009.10.062
  9. Majumdar V, Nagaraja D, Karthik N, Christopher R, (2010). Association of endothelial nitric oxide synthase gene polymorphisms with early-onset ischemic stroke in south Indians. J Atheroscler Thromb, 17, 45-53. https://doi.org/10.5551/jat.1560
  10. Ryk C, Wiklund NP, Nyberg T, de Verdie PJ, (2011). Polymorphisms in nitric-oxide synthase 3 may influence the risk of urinary-bladder cancer. Nitric Oxide, 25, 338-43. https://doi.org/10.1016/j.niox.2011.06.003
  11. Sinici I, Guven EO, Serefoglu E, Hayran M (2010). T-786C Polymorphism in promoter of eNOS gene as genetic risk factor in patients with erectile dysfunction in Turkish population. Urology, 75, 955-60. https://doi.org/10.1016/j.urology.2009.06.063
  12. Soussi T, Lozano G (2005). p53 mutation heterogeneity in cancer. Biochem Biophys Res Commun, 331, 834-42. https://doi.org/10.1016/j.bbrc.2005.03.190
  13. Lin Z, Chen S, Ye C, Zhu S, (2003). Nitric oxide synthase expression in human bladder cancer and its relation to angiogenesis. Urol Res, 31, 232-5. https://doi.org/10.1007/s00240-003-0302-9
  14. Liu S, Gu T, Fu J, et al (2014). Quantum dots-hyperbranched polyether hybrid nanospheres towards delivery and real-time detection of nitric oxide. Materials Science Engineering C, 45, 37-44. https://doi.org/10.1016/j.msec.2014.08.070
  15. Olcay A, Ekmekci CG, Ozbek U, et al (2006). Negative association of endothelial nitric oxide gene polymorphism with hypertension in Turkish patients: effect of ecNOS polymorphism on left ventricular hypertrophy. Cardiovasc Ultrasound, 4, 33. https://doi.org/10.1186/1476-7120-4-33
  16. Safarinejad MR, Safarinejad S, Shafiei N, Safarinejad S, (2013). Effects of the T-786C, G894T, and intron 4 VNTR (4a/b) polymorphisms of the endothelial nitric oxide synthase gene on the risk of prostate cancer. Urol Oncol, 31, 1132-40. https://doi.org/10.1016/j.urolonc.2012.01.002
  17. Thameem F, Puppala S, Arar NH, Stern MP, Blangero J, Duggirala R, Abboud HE, (2008). Endothelial nitric oxide synthase (eNOS) gene polymorphisms and their association with type 2 diabetes-related traits in Mexican Americans. Diab Vasc Dis Res, 5, 109-13. https://doi.org/10.3132/dvdr.2008.018
  18. Verim L, Toptas B, Ozkan NE, et al (2013). Possible relation between the NOS3 gene GLU298ASP polymorphism and bladder cancer in Turkey. Asian Pac J Cancer Prev, 14, 665-8. https://doi.org/10.7314/APJCP.2013.14.2.665
  19. Wu X, Wang Z F, Xu Y, et al (2014). Association between three eNOS polymorphisms and cancer risk: a meta-analysis. Asian Pac J Cancer Prev, 15, 5317-24. https://doi.org/10.7314/APJCP.2014.15.13.5317
  20. Wang XL, Sim AS, Wang MX, et al (2000). Genotype dependent and cigarette specific effects on endothelial nitric oxide synthase gene expression and enzyme activity. FEBS Lett, 471, 45-50. https://doi.org/10.1016/S0014-5793(00)01356-9

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