Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of pH on the Formation of Lysosome-Alginate Beads for Antimicrobial Activity

  • Park, Hyun Jung (Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University) ;
  • Min, Jiho (Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University) ;
  • Ahn, Joo-Myung (Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University) ;
  • Cho, Sung-Jin (Department of Biology, Chungbuk National University) ;
  • Ahn, Ji-Young (Department of Microbiology, Chungbuk National University) ;
  • Kim, Yang-Hoon (Department of Microbiology, Chungbuk National University)
  • Received : 2014.10.13
  • Accepted : 2014.11.19
  • Published : 2015.02.28
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Abstract

In this study, we developed lysosome-alginate beads for application as an oral drug delivery system (ODDS). The beads harboring lysosomes, which have antimicrobial activity, and various concentrations of alginate were characterized and optimized. For application as an ODDS, pH-dependent lysosome-alginate beads were generated, and the level of lysosome release was investigated by using antimicrobial tests. At low pH, lysosomes were not released from the lysosome-alginate beads; however, at neutral pH, similar to the pH in the intestine, lysosome release was confirmed, as determined by a high antimicrobial activity. This study shows the potential of such an ODDS for the in vivo treatment of infection with pathogens.

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References

  1. Alipour S, Montaseri H, Tafaghodi M. 2010. Preparation and characterization of biodegradable paclitaxel loaded alginate microparticles for pulmonary delivery. Colloids Surf. B Biointerfaces 81: 521-529. https://doi.org/10.1016/j.colsurfb.2010.07.050
  2. Das MK, Senapati P. 2008. Furosemide-loaded alginate microspheres prepared by ionic cross-linking technique: morphology and release characteristics. Indian J. Pharm. Sci. 70.
  3. Gurin-Dubiard C, Pasco M, Hietanen A, Quiros del Bosque A, Nau F, Croguennec T. 2005. Hen egg white fractionation by ion-exchange chromatography. J. Chromatogr. A 1090: 58-67. https://doi.org/10.1016/j.chroma.2005.06.083
  4. Jeong SC, Yang BK, Jeong YT, Rao KS, Song CH. 2007. Isolation and characterization of biopolymers extracted from the bark of Acanthopanax sessiliflorus and their anticomplement activity. J. Microbiol. Biotechnol. 17: 21-28.
  5. Nwagu TN, Okolo BN, Aoyagi H. 2012. Stabilization of a raw-starch-digesting amylase by multipoint covalent attachment on glutaraldehyde-activated amberlite beads. J. Microbiol. Biotechnol. 22: 628-636. https://doi.org/10.4014/jmb.1108.08070
  6. Smrdel P, Bogataj M, Mrhar A. 2008. The influence of selected parameters on the size and shape of alginate beads prepared by ionotropic gelation. Sci. Pharm. 76: 77. https://doi.org/10.3797/scipharm.0611-07
  7. Srinivasan S, Jayasree R, Chennazhi K, Nair S, Jayakumar R. 2012. Biocompatible alginate/nano bioactive glass ceramic composite scaffolds for periodontal tissue regeneration. Carbohydr. Polym. 87: 274-283. https://doi.org/10.1016/j.carbpol.2011.07.058
  8. Tønnesen HH, Karlsen J. 2002. Alginate in drug delivery systems. Drug Dev. Ind. Pharm. 28: 621-630. https://doi.org/10.1081/DDC-120003853
  9. Trombetta ES, Ebersold M, Garrett W, Pypaert M, Mellman I. 2003. Activation of lysosom al function during dendritic cell maturation. Science 299: 1400-1403. https://doi.org/10.1126/science.1080106
  10. Wei L, Cai C, Lin J, Wang L, Zhang X. 2011. Degradation of controllable biomaterials constructed from lysozyme-loaded Ca-alginate microparticle/chitosan composites. Polymer 52: 5139-5148. https://doi.org/10.1016/j.polymer.2011.09.006
  11. Wells L, Sheardown H. 2007. Extended release of high p< i> I</i> proteins from alginate microspheres via a novel encapsulation technique. Eur. J. Pharm. Biopharm. 65: 329-335. https://doi.org/10.1016/j.ejpb.2006.10.018
  12. Yoon J, Park JM, Jung SK, Kim KY, Kim YH, Min J. 2009. Characterization of antimicrobial activity of the lysosomes isolated from Saccharomyces cerevisiae. Curr. Microbiol. 59: 48-52. https://doi.org/10.1007/s00284-009-9392-0

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