Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Myxobolus aeglefini (Myxozoa: Myxobolidae) infection in muscles of porous-head eelpout (Bothrocara hollandi)

청자갈치(Bothrocara hollandi)의 근육에 기생하는 점액포자충Myxobolus aeglefini (Myxozoa: Myxobolidae)

  • Jeon, Chan-Hyeok (Department of Marine Bioscience, Gangneung-Wonju National University) ;
  • Kim, Jeong-Ho (Research Institute of East Sea Life Sciences, Gangneung-Wonju National University)
  • 전찬혁 (강릉원주대학교 해양자원육성학과) ;
  • 김정호 (강릉원주대학교 동해안생명과학연구소)
  • Received : 2015.04.08
  • Accepted : 2015.08.10
  • Published : 2015.08.30
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Abstract

A specimen of porous-head eelpout Bothrocara hollandi (Zoarcidae: Perciformes) caught from the East Sea was found to harbour a myxosporean parasite. Numerous whitish pseudocysts were scattered throughout the body musculature of this individual specimen. Fresh myxosporean spores were found from the squashed pseudocysts under light microscopy. They were subspherical in frontal view with a length of $11.9(11.0{\sim}13.5){\mu}m$, width of $11.6(10.7{\sim}13.6){\mu}m$, and thickness of $7.8(6.9{\sim}8.8){\mu}m$. Two polar capsules were almost equally pyriform with a length of $4.4(3.2{\sim}5.3){\mu}m$ and width of $3.3(2.4{\sim}4.2){\mu}m$. Morphometric and host ecology analysis revealed that this myxosporean parasite could be identified as Myxobolus aeglefini Auerbach 1906. Phylogenetic analysis based on 18S rDNA sequences also revealed that M. aeglefini was clustered with M. albi and M. groenlandicus in the same branch, sharing 97.7% and 96.9% sequence similarities with M. albi and M. groenlandicus, respectively.

동해안에서 어획된 청자갈치 (Bothrocara hollandi)의 체측 근육에 유백색의 불투명한 시스트를 형성하고 있는 점액포자충이 발견되었다. 시스트를 마쇄하여 광학현미경으로 관찰해본 결과, 원형에 가까운 점액포자충의 성숙 포자가 관찰되었다. 성숙 포자의 평균 길이는 $11.9(11.0{\sim}13.5){\mu}m$, 평균 폭은 $11.6(10.7{\sim}13.6){\mu}m$, 평균 두께는 $7.8(6.9{\sim}8.8){\mu}m$이었다. 극낭의 평균 길이는 $4.4(3.2{\sim}5.3){\mu}m$이었으며, 평균 폭은 평균 $3.3(2.4{\sim}4.2){\mu}m$이었다. 감염숙주와 성숙포자의 형태학적 특징, 각 부위의 측정값으로부터 본 연구에서 발견된 점액포자충은 Myxobolus aeglefini Auerbach 1906으로 동정하였다. 또한 18S rDNA sequences를 이용한 계통분석 결과 M. albi와 M. groenlandicus와 같은 분지에 속하는 것이 확인되었으며 각각 97.7%와 96.9%의 상동성을 나타내었다.

Keywords

References

  1. Anderson, M.E.: Systematics and osteology of the Zoarcidae (Teleostei: Perciformes). Ichthyol. Bull. J.L.B. Smith Inst. Ichthyol. 60: 120, 1994.
  2. Anderson, M.E. and Fedorov, V.V.: Family Zoarcidae Swainson 1839. Eelpouts. Calif. Acad. Sci. Annotated Checklists of Fishes 34: 58, 2004
  3. Auerbach, M.: Ein Myxobolus im Kopfe von Gadus aeglefinus. Zool. Anz. Leip., 30: 568-570, 1906.
  4. Andree, K.B., Szekely, C., Molnar, K., Gresoviac, S. J. and Hedrick, R.P.: Relationships among members of the genus Myxobolus (Myxozoa: Bivalvulidae) based on small subunit ribosomal DNA sequences. J. Parasitol., 85: 68-74, 1999. https://doi.org/10.2307/3285702
  5. Buchmann, K., Skovgaard, A. and Kania, P.W.: Myxobolus groenlandicus n. sp. (Myxozoa) distorting skeletal structures and musculature of Greenland halibut Reinhardtius hippoglossoides (Teleostei: Pleuronectidae). Dis. Aquat. Org., 98: 133-141, 2012. https://doi.org/10.3354/dao02437
  6. Cavin, J.M., Donahoe, S.L., Frasca, S. Jr., Innis, C.J., Kinsel, M.J., Kurobe, T., Naples, L.M., Nyaoke, A., Poll, C.P. and Weber, E.P.III.: Myxobolus albi infection in cartilage of captive lumpfish (Cyclopterus lumpus). J. Vet. Diagn. Invest., 24: 516-524, 2012. https://doi.org/10.1177/1040638712440990
  7. Choi, J.H., Hong, B.K., Jun, Y.Y., Kim, J.N., Choi, Y.M. and Yoo, O.H.: Feeding comparison of three deepsea fish, Lumpenella longirostris, Malacocottus gibber and Bothrocara hollandi, in the East Sea. J. Kor. Fish. Soc., 42: 151-156, 2009.
  8. Diamant, A., Whipps, C.M. and Kent, M.L.: A new species of Sphaeromyxa (Myxosporea: Sphaeromyxina: Sphaeromyxidae) in devil firefish, Pterois miles (Scorpaenidae), from the northern Red Sea: Morphology, ultrastructure, and phylogeny. J. Parasitol., 90: 1434-1442, 2004. https://doi.org/10.1645/GE-336R
  9. Eiras, J.C., Molnar, K. and Lu, Y.S.: Synopsis of the species of Myxobolus Butschli, 1882 (Myxozoa: Myxosporea: Myxobolidae). Syst. Parasitol., 61: 1-46, 2005. https://doi.org/10.1007/s11230-004-6343-9
  10. Eiras, J.C., Zhang, J. and Molnar, K.: Synopsis of the species of Myxobolus Butschli, 1882 (Myxozoa: Myxosporea, Myxobolidae) described between 2005 and 2013. Syst. Parasitol., 88: 11-36, 2014. https://doi.org/10.1007/s11230-014-9484-5
  11. Felsenstein, J.: PHYLIP (Phylogeny Inference Package) version 3.67. Dept of Genetics, Univ of Washington, Seattle, 2007.
  12. Hallett, S.L. and Diamant, A.: Ultrastructure and smallsubunit ribosomal DNA sequence of Henneguya lesteri n sp. (Myxosporea), a parasite of sand whiting Sillago analis (Sillaginidae) from the coast of Queensland, Australia. Dis. Aquat. Org.. 46: 197-212, 2001. https://doi.org/10.3354/dao046197
  13. Kabata, Z.: Notes on a new host of Myxobolus aeglefini. Parasitology., 27: 165-168, 1956.
  14. Lom, J. and Arthur, J.R.: A guideline for the preparation of species descriptions in Myxosporea. J. Fish Dis., 12: 151-156, 1989. https://doi.org/10.1111/j.1365-2761.1989.tb00287.x
  15. Lom, J. and Dykova, I.: Protozoan parasites of fishes, Developments in Aquaculture and Fisheries Science, 26, Amsterdan, The Netherlands, pp. 204-205, 1992.
  16. Lom, J. and Dykova, I.: Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasitol., 53: 1-36, 2006. https://doi.org/10.14411/fp.2006.001
  17. Molnar K, Eszterbauer E, Szekely C, Dan A. and Harrach B.: Morphological and molecular biological studies on intramuscular Myxobolus spp. of cyprinid fish. J. Fish Dis., 25: 643-652, 2002. https://doi.org/10.1046/j.1365-2761.2002.00409.x
  18. Mackenzie, K. and Kalavati, C.: Mxysporean parasites of marine fishes: their distribution in the world's oceans. Parasitology., 141: 1709-1717, 2014. https://doi.org/10.1017/S0031182014001425
  19. Nielsen, C.V., Koie, M., Szekely, C. and Buchmann, K.: Comparative analysis of 18S rRNA genes from Myxobolus aeglefini Auerbach, 1906 isolated from cod (Gadus morhua), Plaice (Pleuronectes platessa) and dab (Limanda limanda), using PCR-RFLP. Bull. Eur. Ass. Fish. Pathol., 22: 201-205, 2002.
  20. Picon-Camacho, S.M., Holzer, A.S., Freeman, M.A., Morris, D.J. and Shinn, A.P.: Myxobolus albi n. sp. (Myxozoa) from the Gills of the Common Goby Pomatoschistus microps Kroyer (Teleostei: Gobiidae). J. Eukaryot. Microbiol., 56: 421-427, 2009. https://doi.org/10.1111/j.1550-7408.2009.00419.x
  21. Wasko, A.P., Martins, C., Oliverira, C. and Foresti, F.: Non-destructive genetic sampling in fish. An improved method for DNA extraction from fish fins and scales. Hereditas., 138: 161-165, 2003. https://doi.org/10.1034/j.1601-5223.2003.01503.x
  22. Yokoyama, H. and Wakabayashi, S.: Myxobolus aeglefini found in the skeletal muscle of porous head eelpout Allolepis hollandi from the Sea of Japan. Fish. Sci., 66: 963-966, 2000. https://doi.org/10.1046/j.1444-2906.2000.00153.x

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