Journal of Fisheries and Marine Sciences Education (수산해양교육연구)

The Korean Society for Fisheries and Marine Sciences Education (한국수산해양교육학회)
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Characterization of Asymptomatic Megalocytivirus Infection in farmed Rock Fish (Sebastes schlegeli) in Korea

양식 조피볼락 (Sebastes schlegeli)에서 megalocytivirus의 무증상적 감염과 특성 분석

  • Received : 2015.07.16
  • Accepted : 2015.08.14
  • Published : 2015.08.31
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Abstract

Monitoring for megalocytivirus infection was conducted for ten months from March to December in 2013 in 15 aquatic farms culturing, red sea bream, rock bream, rock fish and black sea bream around Tongyoung coastal area in Korea, to assess spatial and temporal variability of detection prevalence, and to explore possible links with seawater temperature. In nested-PCR targeted major capsid protein (MCP) gene, asymptomatic megalocytivirus infection was detected in the externally healthy farmed fish with a significant prevalence in range from 0 to 58.3% for ten months. Higher prevalence of megalocytivirus (46.7% - 57.1%) was observed in high water temperature season from September to November than that in other months with lower prevalence of 0.0% to 20.0%. Even though an acute infection of megalocytivirus was occurred in rock bream (positive in the first PCR) with high mortality in one of fifteen farms, there was no expansion or transmission of the disease to the rock fish and red sea bream culturing in net cage just proximal to the rock bream cage in which disease outbreaked. Nucleotide sequence analysis of the cloned MCP gene isolated asymptomatically infected rock fish revealed that the megalocytivirus in this study was clustered together with the rock bream iridovirus (RBIV) under the subgroup II of the genus megalocytivirus (Iridoviridae), which is known to be the major megalocytivirus strain in Korea. The typical histopathological signs were not found in the spleen of rock fish asymptomatically infected by megalocytivirus. Experimental infection of rock bream with the spleen homogenate of the rock fish infected asymptomatically did not induce any mortality unlike the homogenate of infected rock bream with hih mortlity. However, these results may suggest that the asymptomatic infection of megalocytivirus in other fish species can be a potential risk threatening aquaculture industries as a transmission factor of megalocytivirus to susceptible fish species, especially rock bream.

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References

  1. Chinchar, V. G..Essbauer, S..He, J. G..Hyatt, A. D..Miyazaki, T..Seligy, V. & Williams, T. (2005). Virus taxonomy: classification and nomenclature of viruses : Eighth report of the International Committee on the taxonomy of viruses. 8th edn. Amsterdam. Netherlands, 145-162
  2. Choi, Hye-Sung.Jee, Bo-Young.Cho, Mi-Young and Park, Myoung-Ae(2010). Monitoring of pathogens on the cultured Korean rockfish Sebastes schlegeli in the marine cages farms of south sea area from 2006 to 2008. J. Fish Pathol., 23(1) : 27-35.
  3. He, J. G..Zeng, K..Weng, S. P. & Chan, S. M. (2002). Experimental transmission pathogenicity and physical-chemical properties of infectious spleen and kidney necrosis virus (ISKNV). Aquaculture 204, 11-24. https://doi.org/10.1016/S0044-8486(01)00639-1
  4. Imajoh, M..Ikawa, T. & Oshima S.(2007). Characterization of a new fibroblast cell line from a tail fin of red sea bream. Pagrus major, and phylogenetic relationships of a recent RSIV isolate in Japan. Virus Research. Volume 126, 45-52. https://doi.org/10.1016/j.virusres.2006.12.020
  5. Inouye, K..Yamano, K..Maeno, Y..Nakajima, K..Matsuoka, M..Wada, Y. & Sorimachi, M. (1992). Iridovirus infection of cultured Red Sea Bream Pagrus major. Gyobyo Kenkyu, 27(1), 19-27. https://doi.org/10.3147/jsfp.27.19
  6. Jeong, Hyun-Do & Jeong, Joon-Bum(2010). Presence of low level infected iridovirus in sea perch Lateolabrax sp. imported to Korea. J. Fish Pathol., 23(1), 17-25.
  7. Jeong, Joon-Bum & Jeong, Hyun-Do(2008). Pathogenicity of Iridovirus against Marine Fish and Its Detection in Culturing Seawater. J. Kor. Fish. Soc. 41(1), 20-25.
  8. Jeong, Joon-Bum.Jun, Lyu-Jin.Park, Kyung- Hyun.Kim Ki-Hong.Chung, Joon-Ki.Komisar, J. L..Jeong, Hyun-Do(2006). Asymptomatic iridovirus infection in various marine fishes detected by a 2-step PCR method. Aquaculture 255, 30-38. https://doi.org/10.1016/j.aquaculture.2005.12.019
  9. Joh, Seong-Joon.Kim, Doo-Won.Kim, Jeong-Ho & Heo, Gang-Joon(2000). Dtection of Marine Birnavirus (MBV) from Rockfish Sebastes schlegeli Using Reverse Transcription and Nested PCR. J. Microbiology, 260-264.
  10. Jung, Sung-Ju & Oh, Myung-Joo(2000). Iridovirus-like infection associated with high mortalities of striped beakperch, Oplegnathus fasciatus (Temminck et Schlegel) in southern coastal areas of the Korean peninsula. J. Fish Dis., 23, 223-226. https://doi.org/10.1046/j.1365-2761.2000.00212.x
  11. Jung, Sung-Ju.Miyazaki, T..Miyata, M..Danayadol, Y. & Tanaka, S.(1991). Pathogenicity of Iridovirus from Japan and Thailand for the red sea bream Pagrus major in Japan, and histopathology of experimentally infected fish. Fish. Sci., 63(5) : 735-740.
  12. Kawakami, H. & Nakajima, K.(2002). Cultured fish species affected by red sea bream iridoviral disease from 1996 to 2000. J. Fish Pathol. 37, 45-47. https://doi.org/10.3147/jsfp.37.45
  13. Kim, Jin-Woo.Cho, Mi-Young.Jin Ji-Woong, Kim, Ki-Hong.Jeong, Hyun-Do & Kim, Kwang-Il (2011). Detection of Megalocytivirus in shellfish using PCR with various DNA extraction methods. J Fish Pathol 24, 65-73. https://doi.org/10.7847/jfp.2011.24.2.065
  14. Kim, Seok-Ryel.Jeong, Byeong-Mum.Jung SSung-Ju.Kitamura, S. I..Kim, Du-Woon.Kim, Do-Hyung & Oh, Myung. Joo.(2008). Cumulative Mortality in Striped Beakperch Oplegnathus fasciatus Infected with Red Sea Bream Iridovirus (RSIV) at Different Water Temperature and Identification of Heat Shock Protein 70. J. Fish Pathol. 21(1), 13-20
  15. Kim, Young-Jin.Jung, Sung-Ju.Choi, Tae-Jin.Kim, Hyeong-Rak.Rajendran, K.V.& Oh, Myung-Joo (2002). PCR amplification and sequence analysis of irido-like virus infecting fish in Korea. J. Fish Dis., 25, 121-124. https://doi.org/10.1046/j.1365-2761.2002.00345.x
  16. KOSTAT(2013). Statistical yearbook of maritime affairs & fisheries. Fishery production by fishery sector fishery type and province. 83-84.
  17. Matsuoka, S..Inouye, K. & Nakajima, K.(1996). Cultured fish species affected by red sea bream iridoviral disease from 1991 to 1995. J. Fish Pathol. 31, 233-234. https://doi.org/10.3147/jsfp.31.233
  18. Office International des Epizooties(2009). Manual of diagnostic tests for aquatic animals
  19. Sohn, Sang-Gyu.Park, Myoung-Ae.Oh, Myung-Joo & Chun, Seh-Kyu(1998). A fish nodavirus isolated from cultured sevenband grouper, Epinephelus septemfasciatus. Journal of Fish Pathol., 11, 97-104.
  20. Wang, Y. Q..Lu, L..Weng, S. P..Huang, J. N..Chan, S. M. and He, J. G.(2007). Molecular epidemiology and phylogenetic analysis of a marine fish infectious spleen and kidney necrosis virus-like (ISKNV-like) virus. Arch Virol 152, 763-773. https://doi.org/10.1007/s00705-006-0870-4

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