Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Sedative-Hypnotic and Receptor Binding Studies of Fermented Marine Organisms

  • Joung, Hye-Young (Department of Physiology, College of Medicine, The Catholic University of Korea) ;
  • Kang, Young Mi (Marine Bioprocess Co., Ltd.) ;
  • Lee, Bae-Jin (Marine Bioprocess Co., Ltd.) ;
  • Chung, Sun Yong (Department of Oriental Neuropsychiatry, Kyung Hee University Korean Medicine Hospital at Gangdong) ;
  • Kim, Kyung-Soo (Department of Family Medicine, College of Medicine, The Catholic University of Korea) ;
  • Shim, Insop (Department of Science in Korean Medicine, Graduate School, College of Korean Medicine, Kyung Hee University)
  • Received : 2014.10.23
  • Accepted : 2015.07.03
  • Published : 2015.09.01
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Abstract

This study was performed to investigate the sedative-hypnotic activity of ${\gamma}$-aminobutyric acid (GABA)-enriched fermented marine organisms (FMO), including sea tangle (FST) and oyster (FO) by Lactobacillus brevis BJ20 (L. brevis BJ20). FST and FO were tested for their binding activity of the $GABA_A$-benzodiazepine and 5-$HT_{2C}$ receptors, which are well-known molecular targets for sleep aids. We also measured the sleep latency and sleep duration during pentobarbital-induced sleep in mice after oral administration of FST and FO. In $GABA_A$ and 5-$HT_{2C}$ receptor binding assays, FST displayed an effective concentration-dependent binding affinity to $GABA_A$ receptor, similar to the binding affinity to 5-$HT_{2C}$ receptor. FO exhibited higher affinity to 5-$HT_{2C}$ receptor, compared with the $GABA_A$ receptor. The oral administration of FST and FO produced a dose-dependent decrease in sleep latency and increase in sleep duration in pentobarbital-induced hypnosis. The data demonstrate that FST and FO possess sedativehypnotic activity possibly by modulating $GABA_A$ and 5-$HT_{2C}$ receptors. We propose that FST and FO might be effective agents for treatment of insomnia.

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References

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