Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Gastroprotective Activities of Sennoside A and Sennoside B via the Up-Regulation of Prostaglandin E2 and the Inhibition of H+/K+-ATPase

  • Received : 2015.05.04
  • Accepted : 2015.05.28
  • Published : 2015.09.01
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Abstract

Sennoside A (erythro) and sennoside B (threo) are dianthrone glycosides and diastereomers. We investigated their abilities to prevent the gastric lesions associated with diseases, such as, gastritis and gastric ulcer. To elucidate their gastroprotective effects, the inhibitions of $HCl{\cdot}tOH$-induced gastritis and indomethacin-induced gastric ulcers were assessed in rats. It was observed that both sennoside A and sennoside B increased prostaglandin $E_2$ ($PGE_2$) levels and inhibited $H^+/K^+$-ATPase (proton pump). In a rat model, both compounds reduced gastric juice, total acidity and increased pH, indicating that proton pump inhibition reduces gastric acid secretion. Furthermore, sennoside A and B increased $PGE_2$ in a concentration-dependent manner. In a gastric emptying and intestinal transporting rate experiment, both sennoside A and sennoside B accelerated motility. Our results thus suggest that sennoside A and sennoside B possess significant gastroprotective activities and they might be useful for the treatment of gastric disease.

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References

  1. Araki, H., Ukawa, H., Sugawa, Y., Yagi, K., Suzuki, K. and Takeuchi, K. (2000) The roles of prostaglandin E receptor subtypes in the cytoprotective action of prostaglandin $E_2$ in rat stomach. Aliment. Pharmacol. Ther. 14, 116-124 . https://doi.org/10.1046/j.1365-2036.2000.014s1116.x
  2. Ashley, S. W., Sonnenschein, L. A. and Cheung, L. Y. (1985) Focal gastric mucosal blood flow at the ite of aspirin-induced ulceration. Am. J. Surg. 149, 53-59. https://doi.org/10.1016/S0002-9610(85)80009-X
  3. Bandyopadhyay, U1, Biswas, K., Chatterjee, R., Bandyopadhyay, D., Chattopadhyay, I., Ganguly, C. K., Chakraborty, T., Bhattacharya, K. and Banerjee, R. K. (2002) Gastroprotective effect of Neem (Azadirachta indica) bark extract: Possible involvement of $H^+$/$K^+$-ATPase inhibition and scavenging of hydroxyl radical. Life Sci. 71, 2845-2865. https://doi.org/10.1016/S0024-3205(02)02143-4
  4. Basson, M. D., Modlin, I. M., Flynn, S. D., Jena, B. P. and Madri, J. A. (1992) Independent modulation of enterocyte migration and proliferation by growth factors, matrix proteins and pharmacologic agents in an in vitro model of mucosal healing. Surgery 112, 299-307.
  5. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of crogram quantities of protein utilizing the principle of proteindye binding. Anal. Biochem.72, 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  6. Chen, Y. C., Chang, C. N., Hsu, H. C., Chiou, S. J., Lee, L. T., Hseu, T. H. (2009) Sennoside B inhibits PDGF receptor signaling and cell proliferation induced by PDGF-BB in human osteosarcoma cells. Life Sci. 84, 915-922. https://doi.org/10.1016/j.lfs.2009.04.003
  7. Karmeli, F., Eliakim, R., Okon, E. and Rachmilewitz, D. (1995) Ketotifen and nitroxides decrease capsaicin-augmented ethanol-induced gastric damage in rats. Dig. Dis. Sci. 40, 1140-1146. https://doi.org/10.1007/BF02064212
  8. Kasuya, Y., Urushidani, T. and Okabe, S. (1979) Effects of various drugs and vagotomy on indomethacin-induced gastric ulcers in the rat. Jpn. J. Pharmacol. 29, 670-673. https://doi.org/10.1254/jjp.29.670
  9. Kato, S., Morie, T. and Yoshida, N. (1995) Synthesis and biological activities of metabolites of mosapride, a new gastroprokinetic agent. Chem. Pharm. Bull. 43, 699-702. https://doi.org/10.1248/cpb.43.699
  10. Kim, B. J., Jung, H. J., Hwang, J. N., Kang, S. H., Oh, S. J. and Seo, Y. R. (2004) Overview on molecular toxicological aspects of Helicobacter pylori virulence factor, cytotoxin-associated antigen A (CagA). J. Toxicol. Pub. Health 20, 179-185.
  11. Kimmey, M. B. (1992) NSAID, ulcers and prostaglandins. J. Rheumatol. Suppl. 36, 68-73.
  12. Kobayashi, M., Yamaguchi, T., Odaka, T., Nakamura, T., Tsuchiya, S., Yokosuka, O., Yano S. (2007) Regionally differential effects of sennoside a on spontaneous contractions of colon in mice. Basic Clin. Pharmacol. Toxicol. 101, 121-126. https://doi.org/10.1111/j.1742-7843.2007.00088.x
  13. Konturek, P. C., Brzozowski, T., Duda, A., Kwiecien, S., Lober, S., Dembinski, A., Hahn, E. G. and Konturek, S. J. (2001) Epidermal growth factor and prostaglandin $E_2$ accelerate mucosal recovery from stress-induced gastric lesions via inhibition of apoptosis. J. Physiol. Paris 95, 361-367. https://doi.org/10.1016/S0928-4257(01)00049-3
  14. Konturek, S. J., Kwiecien, N., Obtulowicz, W., Polanski, M., Kopp, B. and Oleksy, J. (1983) Comparison of prostaglandin $E_2$ and ranitidine in prevention of gastric bleeding by aspirin in man, Gut 24, 89-93. https://doi.org/10.1136/gut.24.2.89
  15. Lee, J. S., Oh, T. Y., Kim, Y. K., Baik, J. H. and So, S. (2005) Protective effects of green tea polyphenol extracts against ethanol-induced gastric mucosal damages in rats: Stress-responsive transcription factors and MAP kinases as potential targets. Mutat. Res. 579, 214-224. https://doi.org/10.1016/j.mrfmmm.2005.03.027
  16. Mcquaid, K. R. and Isenberg, J. I. (1992) Medical therapy of peptic ulcer disease. Surg. Clin. North Am. 72, 285-316 .
  17. Mizui, T. and Dodeuchi, M. (1983) Effect of polyamines on acidified ethanol induced gastric lesion in rats. Jpn. J. Pharmacol. 33, 939-945. https://doi.org/10.1254/jjp.33.939
  18. Nandi, J., Das, P. K., Zinkievich, J. M., Baltodano, J. D. and Levine, R. A. (2009) Cyclo-oxygenase-1 inhibition increases acid secretion by modulating $H^+$/$K^+$-ATPase expression and activation in rabbit parietal cells. Clin. Exp. Pharmacol. Physiol. 36, 127-134. https://doi.org/10.1111/j.1440-1681.2008.05032.x
  19. Odaka, T., Suzuki, T., Seza, A., Yamaguchi, T. and Saisho, H. (2006) Serotonin 5-$HT_4$ receptor agonist (mosapride citrate). Nippon Rinsho 64, 1491-1494 .
  20. Okabe, S., Takeuchi, K., Nakamura, K. and Takagi, K. (1974) Pathogenesis of gastric lesions induced by aspirin in the pylorus-lygated rat. Jpn. J. Pharmacol. 24, 363-371. https://doi.org/10.1254/jjp.24.363
  21. Raghunath, A. S., O'Morain, C. and Mcloughlin, R. C. (2005) The longterm use of proton-pump inhibitors. Aliment. Pharmacol. 22, 55-63 . https://doi.org/10.1111/j.1365-2036.2005.02611.x
  22. Rao, C. V., Ojha, S. K., Radhakrishnan, K., Govindarajan, R., Rastogi, S., Mehrotra, S. and Pushpangadan, P. (2004) Antiulcer activity of Utleria salicifolia rhizome extract. J. Ethnopharmacol. 91, 243-249. https://doi.org/10.1016/j.jep.2003.12.020
  23. Rovert, A. (1979) Cytoprotection by prostaglandins. Gastroenterology 77, 761-767.
  24. Rovert, A., Nezaims, J. E., Lancaster, C. and Hanchar, A. J. (1979) Cytoprotection by prostaglandins in rats; Prevention of gastric necrosis produced by alcohol, HCl, NaOH, hypertonic NaCl and thermal injury. Gastroenterology 77, 433-443.
  25. Ruppin, H., Person, B., Rober, A. and Domschke, W. (1981) Gastric cytoprotection in man by prostaglandin $E_2$. Scand. J. Gastroenterol. 16, 647-652 . https://doi.org/10.3109/00365528109182025
  26. Saccomani, G., Barcellona, M. L. and Sachs, G. (1981) Reactivity of gastric ($H^+$/$K^+$)-ATPase to N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline. J. Biol. Chem. 256, 12405-12410.
  27. Scarpignato, C., Capovilla, T. and Bertaccini, G. (1980) Action of caerulein on gastric emptying of the conscious rat. Arch. Int. Pharmacodyn. Ther. 246, 286-294.
  28. Seiki, M., Ueki, S., Tanaka, Y., Soeda, M., Hori, Y., Aita, H., Yoneta, T., Morita, H., Tagashira, E. and Okabe, S. (1990) Studies on antiulcer effects of a new compound, zinc L-carnosine (Z-103). Nihon Yakurigaku Zasshi 95, 257-269. https://doi.org/10.1254/fpj.95.5_257
  29. Sekiguchi, F., Saito, S., Takaoka, K., Hayashi, H., Nagataki, M., Nagasawa, K., Nishikawa, H., Matsui, H. and Kawabata, A. (2007) Mechanisms for prostaglandin $E_2$ formation caused by proteinaseactivated receptor-1 activation in rat gastric mucosal epithelial cells. Biochem. Pharmacol. 73, 103-114. https://doi.org/10.1016/j.bcp.2006.09.016
  30. Shay, H., Komarov, S. A., Fels, S. S. and Meranze, D. (1945) A simple method for the uniform production of gastric ulceration in the rat. Gastroenterology 5, 43-61.
  31. Takemori, A. E., Kupferberg, H. T. and Miller, J. W. (1969) Quantitative studies of antagonism of morphine by nalorphine and naloxone. J. Pharmacol. Exp. Ther. 169, 39-45.
  32. Walker, J., Hell, J., Liszt, K. I., Dresel, M., Pignitter, M., Hofmann, T. and Somoza, V. (2012) Identification of beer bitter acids regulating mechanisms of gastric acid secretion. J. Agric. Food Chem. 60, 1405-1412. https://doi.org/10.1021/jf204306z
  33. Wallace, J. L. and Granger, D. N. (1992) Pathogenesis of NSAID gastropathy: are neutrophils the culprits? Trends Pharmacol. Sci. 13, 129-131. https://doi.org/10.1016/0165-6147(92)90046-9
  34. Watson, C., Zhu, L., Guan, S., Machen, T. E. and Forte, J. G. (2013) Reaction of proton pump inhibitors with model peptides results in novel products. J. Pharmacol. Sci. 122, 213-222. https://doi.org/10.1254/jphs.13058FP
  35. Zhang, Y., Chen, H. and Yang L. (2010) Toll-like receptor 4 participates in gastric mucosal protection through Cox-2 and PG$E_2$. Dig. Liver Dis. 42, 472-476. https://doi.org/10.1016/j.dld.2009.10.007

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