한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제23권2호
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  • Pages.138-143
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  • 2015
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
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마우스 수컷 생식세포에서 비스페놀 A에 대한 인삼 에탄올 추출물의 보호 효과

Protective Effect of Panax ginseng Ethanol Extracts Against Bisphenol A (BPA) in Mouse Male Germ Cells

  • 김형돈 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 손상현 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김진성 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이희정 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 박춘근 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 안영섭 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이상원 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영옥 (농촌진흥청 국립원예특작과학원 인삼특작부)
  • 투고 : 2014.10.01
  • 심사 : 2015.04.06
  • 발행 : 2015.04.28
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초록

This study was carried out to evaluate the preventive effect of three forms of Korean ginseng roots (fresh, white and red) against bisphenol A (BPA) toxicity in mouse male germ cells (GC-2spd, TM3, TM4). ROS (reactive oxygen species) generation were measured by DCF-DA (2',7'-dichlorohydrofluorescein diacetate) assay. Also, semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was performed to quantify the mRNA expression levels of apoptosis-related genes, Bax (pro-apoptotic gene) and Bcl2 (anti-apoptotic gene). ROS generation was increased by $50{\mu}M$ BPA, but definitely decreased by treatment with Korean ginseng extracts (fresh, white and red) in mouse male germ cells. In especial, Korean fresh ginseng extract reduced significantly ROS production to normal control. In addition, Korean fresh and white ginseng extracts suppressed the apoptosis of mouse male germ cells by fine-tuning mRNA levels of apoptotic genes changed by BPA. In general, Korean fresh ginseng extract was more effective than white ginseng extract for reducing BPA-induced oxidative stress and apoptosis in mouse male germ cells. Therefore, Korean fresh and white ginseng may help to alleviate biphenol A toxicity in mouse male germ cells.

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참고문헌

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