대한한의학회지 (The Journal of Korean Medicine)

  • 제36권2호
  • /
  • Pages.21-35
  • /
  • 2015
  • /
  • 1010-0695(pISSN)
  • /
  • 2288-3339(eISSN)
대한한의학회 (The Society of Korean Medicine)
권호 표지
DOI QR코드

DOI QR Code

Effect of Picrorrhizae Rhizoma Aqueous Extracts on Paw Chronic Inflammation In Mice

  • Leem, Ji-Young (Department of Herbology, College of Oriental Medicine, Daegu Hanny University) ;
  • Ku, Sae-Kwang (Department of Anatomy and Histoloty, College of Oriental Medicine, Daegu Hanny University) ;
  • Seo, Bu-Il (Department of Herbology, College of Oriental Medicine, Daegu Hanny University) ;
  • Jeon, Mi-Hye (Department of Herbology, College of Oriental Medicine, Daegu Hanny University) ;
  • Park, Ji-Ha (Department of Herbology, College of Oriental Medicine, Daegu Hanny University)
  • 투고 : 2015.05.25
  • 심사 : 2015.06.19
  • 발행 : 2015.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives: The purpose of this study was to examine the efficacy of Picrorrhizae Rhizoma (PR) aqueous extracts on the formalin-induced paw chronic inflammation in mice. Methods: PR extracts (500, 250 and 125mg/kg) or distilled water (DW) were orally administered once a day for 10 days to formalin-injected chronic inflammatory mice. The paw thicknesses and volumes were measured daily and the paw wet-weight and histological profiles were conducted at termination with paw tumor necrosis factor (TNF)-${\alpha}$ contents measurement. The anti-inflammatory effects of PR extracts were compared with dexamethasone. Results: In DW treated control group, the paw thickness, paw wet-weights and paw TNF-${\alpha}$ contents were markedly increased. Severe chronic inflammation signs such as severe fibrosis, the formation of necrotic debris, and infiltration of inflammatory cells were detected in histopathological observations. However, these formalin-induced changes were dramatically decreased by treatment of dexamethasone and all three different dosages of PR extracts. The anti-inflammatory effects of PR at highest dose were slighter than that of dexamethasone, but it did not show any harmful effects on the body weight contrary to dexamethasone. Conclusion: These results suggest that PR extracts have safe and favorable effects on formalin-induced chronic inflammation.

키워드

참고문헌

  1. Habashy RR, Abdel-Naim AB, Khalifa AE, Al-Azizi MM. Anti-inflammatory effects of jojoba liquid wax in experimental models. Pharmacol Res. 2005;51:95-105. https://doi.org/10.1016/j.phrs.2004.04.011
  2. Kim HD, Cho HR, Moon SB, Shin HD, Yang KJ, Park BR, et al. Effect of exopolymers from Aureobasidum pullulans on formalin-induced chronic paw inflammation in mice. J MicrobiolBiotechnol. 2006;16:1954-1960.
  3. Kim HD, Cho HR, Moon SB, Shin HD, Yang KJ, Park BR, et al. Effects of ${\beta}$-glucan from Aureobasidum pullulans on acute inflammation in mice. Arch Pharm Res. 2007;30:323-328. https://doi.org/10.1007/BF02977613
  4. Hosseinzadeh H, Younesi HM. Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice. BMC Pharmacol. 2002;2:7-12.
  5. Pillai AD, Pathod PD, Franklin PX, Patel M, Nivsarkar M, Vasu KK, et al. Novel drug designing approach for dual inhibitors as anti-inflammatory agents: implication of pyridine template. BiochemBiophys Res Commun. 2003; 301:183-186. https://doi.org/10.1016/S0006-291X(02)02996-0
  6. Abdel-Salam OM, Baiuomy AR, El-batran S, Arbid MS. Evaluation of the anti-inflammatory, anti-nociceptive and gastric effects of Ginkgo biloba in the rat. Pharmacol Res. 2004;49:133-142. https://doi.org/10.1016/j.phrs.2003.08.004
  7. Akindele AJ, Adeyemi OO. Anti-inflammatory activity of the aqueous leaf extract of Byrsocarpus coccineus. Fitoterapia. 2007;78: 25-28. https://doi.org/10.1016/j.fitote.2006.09.002
  8. Schafers M, Lee DH, Brors D, Yaksh TL, Sorkin LS. Increased sensitivity of injured and adjacent uninjured rat primary sensory neurons to exogenous tumor necrosis factor-alpha after spinal nerve ligation. J Neurosci. 2003;23:3028-3038. https://doi.org/10.1523/JNEUROSCI.23-07-03028.2003
  9. Sorkin LS, Xiao WH, Wagner R, Myers RR. Tumour necrosis factor-alpha induces ectopic activity in nociceptive primary afferent fibres. Neuroscience. 1997;81:255-262. https://doi.org/10.1016/S0306-4522(97)00147-4
  10. Watkins LR, Maier SF. Beyond neurons: evidence that immune and glial cells contribute to pathological pain states. Physiol Rev. 2002 ;82:981-1011. https://doi.org/10.1152/physrev.00011.2002
  11. Bianchi M, Martucci C, Biella G, Ferrario P, Sacerdote P. Increased substance P and tumor necrosis factor-alpha level in the paws following formalin injection in rat tail. Brain Res. 2004;1019:255-258. https://doi.org/10.1016/j.brainres.2004.06.007
  12. Wang C, Schuller Levis GB, Lee EB, Levis WR, Lee DW, Kim BS, et al. Platycodin D and D3 isolated from the root of Platycodon grandiflorum modulate the production of nitric oxide and secretion of TNF-alpha in activated RAW 264.7 cells. IntImmunopharmacol. 2004;4: 1039-1049.
  13. Gupta AK, Chow M. Prednicarbate (Dermatop): profile of a corticosteroid. J Cutan Med Surg. 2004;8:244-247. https://doi.org/10.1007/s10227-004-0120-x
  14. Chi YM, Nakamura M, Zhao AY, Yoshizawa T, Yan WM, Hashimoto F, et al. Anti-inflammatory activity of 4, 4'-dihydroxy-alpha-truxillic acid. Biol Pharm Bull. 2006;29:489-493. https://doi.org/10.1248/bpb.29.489
  15. Jagetia GC, Baliga MS. The evaluation of nitric oxide scavenging activity of certain Indian medicinal plants in vitro: a preliminary study. J Med Food. 2004;7:343-348. https://doi.org/10.1089/jmf.2004.7.343
  16. Senthil Kumar SH, Anandan R, Devaki T, Santhosh Kumar M. Cardioprotective effects of Picrorrhiza kurroa against isoproterenol-induced myocardial stress in rats. Fitoterapia. 2001;72: 402-405. https://doi.org/10.1016/S0367-326X(01)00264-7
  17. Jeena KJ, Joy KL, Kuttan R. Effect of Emblica officinalis, Phyllanthus amarus and Picrorrhiza kurroa on N-nitrosodiethylamine induced hepatocarcinogenesis. Cancer Lett. 1999;136: 11-16. https://doi.org/10.1016/S0304-3835(98)00294-8
  18. Joy KL, Rajeshkumar NV, Kuttan G, Kuttan R. Effect of Picrorrhiza kurroa extract on transplanted tumours and chemical carcinogenesis in mice.J Ethnopharmacol. 2000;71:261-266. https://doi.org/10.1016/S0378-8741(00)00168-9
  19. Joy KL, Kuttan R. Anti-diabetic activity of Picrorrhiza kurroa extract. J Ethnopharmacol. 1999;67:143-148. https://doi.org/10.1016/S0378-8741(98)00243-8
  20. Mehrotra R, Rawat S, Kulshreshtha DK, Patnaik GK, Dhawan BN. In vitro studies on the effect of certain natural products against hepatitis B virus. Indian J Med Res. 1990;92:133-138.
  21. Atal CK, Sharma ML, Kaul A, Khajuria A. Immunomodulating agents of plant origin. I: Preliminary screening. J Ethnopharmacol. 1986; 18:133-141. https://doi.org/10.1016/0378-8741(86)90025-5
  22. Puri A, Saxena RP, Guru PY, Kulshreshtha DK, Saxena KC, Dhawan BN. Immunostimulant activity of Picroliv, the iridoid glycoside fraction of Picrorhiza kurroa, and its protective action against Leishmania donovani infection in hamsters1. Planta Med. 1992;58:528-532. https://doi.org/10.1055/s-2006-961542
  23. Sharma ML, Rao CS, Duda PL. Immunostimulatory activity of Picrorhiza kurroa leaf extract.J Ethnopharmacol. 1994;41:185-192. https://doi.org/10.1016/0378-8741(94)90031-0
  24. Lee HS, Ahn HC, Ku SK. Hypolipemic effect of water extracts of Picrorrhizae Rhizoma in PX-407 induced hyperlipemic ICR mouse model with hepatoprotective effects: A prevention study. J Ethnopharmacol. 2006a;105:380-386. https://doi.org/10.1016/j.jep.2005.11.010
  25. Lee HS, Yoo CB, Ku SK. Hypolipemic effect of water extracts of Picrorrhizae Rhizoma in high fat diet treated mouse. Fitoterapia. 2006c; 77:579-584. https://doi.org/10.1016/j.fitote.2006.09.004
  26. Lee HS, Woo SJ, Ku SK. Hypolipemic and hepatoprotective effects of Picrorrhizae Rhizoma in high fat diet supplied mice. A prevention study. Biomolecules & Therapeutics. 2008;16: 46-53. https://doi.org/10.4062/biomolther.2008.16.1.046
  27. Lee HS, Ku SK. Effects of Picrorrhizae Rhizoma on acute inflammation in mice. Biomolecules & Therapeutics. 2008;16:137-140. https://doi.org/10.4062/biomolther.2008.16.2.137
  28. Park JH, Lee SN, Ku SK. Effects of Picrorrhizae Rhizoma on dinitroflurobenzene-induced contact dermatitis (Type I allergy). Biomolecules & Therapeutics. 2008;16:237-242. https://doi.org/10.4062/biomolther.2008.16.3.237
  29. Lee HS, Lee IK, Ku SK. Single oral dose toxicity study of Water Extracts of Picrorrhizae Rhizoma in ICR mice. J Toxicol Pub Health. 2006b;22:117-126.
  30. Di Renzo L, Yefenof E, Klein E. The function of human NK cells is enhanced by beta-glucan, a ligand of CR3 (CD11b/CD18). Eur J Immunol. 1991;21:1755-1758. https://doi.org/10.1002/eji.1830210726
  31. Ross GD, Vetvicka V, Yan J, Xia Y, Vetvickova J. Therapeutic intervention with complement and beta-glucan in cancer. Immunopharmacology 1999;42:61-74. https://doi.org/10.1016/S0162-3109(99)00013-2
  32. Ramprasath VR, Shanthi P, Sachdanandam P. Immunomodulatory and anti-inflammatory effects of Semecarpus anacardium LINN. Nut milk extract in experimental inflammatory conditions. Biol Pharm Bull. 2006;29:693-700. https://doi.org/10.1248/bpb.29.693
  33. Amin AR, Vyas P, Attur M, Leszczynska-Piziak J, Patel IR, Weissmann G, et al. The mode of action of aspirin-like drugs: effect on inducible nitric oxide synthase. Proc Natl AcadSci USA. 1995;92:7926-7930. https://doi.org/10.1073/pnas.92.17.7926
  34. Beutler B, Greenwald D, Hulmes JD, Chang M, Pan YC, Mathison J, et al. Identity of tumor necrosis factor and the macrophage-secreted factor cachectin. Nature. 1985;316:552-554. https://doi.org/10.1038/316552a0
  35. Whittle BJ, Varga C, Berko A, Horvath K, Posa A, Riley JP, et al. Attenuation of inflammation and cytokine production in rat colitis by a novel selective inhibitor of leukotriene A4 hydrolase. Br J Pharmacol. 2008;153:983-991.
  36. Qi XF, Kim DH, Yoon YS, Li JH, Jin D, Deung YK, et al. Effects of Bambusae caulis in Liquamen on the development of atopic dermatitis-like skin lesions in hairless mice. J Ethnopharmacol. 2009;123:195-200. https://doi.org/10.1016/j.jep.2009.03.020
  37. Schottelius AJ, Zügel U, Döcke WD, Zollner TM, Röse L, Mengel A, et al. The role of mitogen-activated protein kinase-activated protein kinase 2 in the p38/TNF-alpha pathway of systemic and cutaneous inflammation. J Invest Dermatol. 2010;130:481-491. https://doi.org/10.1038/jid.2009.218
  38. Assuma R, Oates T, Cochran D, Amar S, Graves DT. IL-1 and TNF antagonists inhibit the inflammatory response and bone loss in experimental periodontitis. J Immunol. 1998; 160:403-409.
  39. Plata EJ, Murphy WH. Growth and haematologic properties of the BALB/wm strain of inbred mice. Lab Anim Sci. 1972;22:712-720.
  40. Yamaguchi C, Fujita S, Obara T, Ueda T. Effects of room temperature on reproduction, body weight and organ weights, food and water intakes, and hematology in mice. Exp Anim. 1983;32:1-11. https://doi.org/10.1538/expanim1978.32.1_1