Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Production of Hypoallergenic Cocoa Beans by a Pregermination Treatment Method

  • Handoyo, Tri (Plant Physiology Laboratory, Faculty of Agriculture, Jember University / Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Mitsugu, Akagawa (Department of Biological Chemistry, Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University) ;
  • Kim, Kyung-Min (Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University)
  • Received : 2015.05.15
  • Accepted : 2015.06.02
  • Published : 2015.06.30
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Abstract

Pregermination treatment of cocoa beans either with the testa, group PCB (+T), or without the testa, group PCB (-T), was studied here to determine whether this treatment (incubation up to 120 h at $25^{\circ}C$) has any effect on the levels of allergenic proteins or on chemical composition. Our proximate analysis included carbohydrates, proteins, and lipids. We used western blotting to measure changes in the amounts of allergenic proteins in the cocoa beans during the pregermination treatment. The protein and carbohydrate content of both groups (with or without the testa) decreased slightly during this treatment, whereas lipid content increased. Group PCB (-T) showed more rapid metabolic processes than did group PCB (+T) during the pregermination treatment. Western blot analysis showed that the cocoa beans contained an allergenic protein of ~28 kDa. Removal of the testa strongly reduced the amount of this allergenic protein after 72 h of the pregermination treatment. Generally, the pregermination treatment increased antioxidant activity in both groups. Significant differences in the antioxidant activity were observed between groups PCB (-T) and PCB (+T). Particularly, group PCB (-T) showed high antioxidant activity at 72 h of the pregermination treatment. Thus, the combination of cocoa beans without the testa and pregermination treatment (72 h) seems to be the optimal method for production of hypoallergenic cocoa beans rich in antioxidants for patients with allergic disorders.

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References

  1. Abril-Gil M, Massot-Cladera M, Perez-Cano FJ, Castellote C, Franch A, Castell M (2012) A diet enriched with cocoa prevents IgE synthesis in a rat allergy model. Pharmacological Research 65:603-608 https://doi.org/10.1016/j.phrs.2012.02.001
  2. Afoakwa EO, Paterson A, Fowler M (2007) Factors influencing rheological and textural qualities in chocolate-a review. Trends in Food Science and Technology 18:290-298 https://doi.org/10.1016/j.tifs.2007.02.002
  3. Akagawa M, Handoyo T, Ishii T, Kumazawa S, Morita N, Suyama K (2007) Proteomic analysis of wheat flour allergens. Journal Agricultural and Food Chemistry 22:6863-6870
  4. Arlorio M, Coisson JD, Travaglia F, Varsaldi F, Miglio G, Lombardi G (2005) Antioxidant and biological activity of phenolic pigments from Theobroma cacao hulls extracted with supercritical $CO_2$. Food Research International 38: 1009-1014 https://doi.org/10.1016/j.foodres.2005.03.012
  5. Association of Official Analytical Chemists (2002) Official methods of analysis of AOAC International. Arlington: AOAC International
  6. Belscak A, Komes D, Horzic D, Ganic KK (2009) Comparative study of commercially available cocoa products in terms of their bioactive composition. Food Research International 42: 707-716 https://doi.org/10.1016/j.foodres.2009.02.018
  7. Borchers AT, Keen CL, Hannum SM, Gershwin ME (2004) Cocoa and Chocolate: composition, bioavailability, and health implications. Journal of Medicinal Food 3:77-103
  8. Brand-Williams W, Cuvelier ME, Berset C (1995) Use of free radical method to evaluate antioxidant activity. LWT Food Science and Technology 28:25-30 https://doi.org/10.1016/S0023-6438(95)80008-5
  9. Demeule M, Michaud-Levesque J, Annabi B, Gingras D, Boivin D, Jodoin J, Lamy S, Bertrand Y, Beliveau R (2002) Green tea catechins as novel antitumor and antiangiogenic compounds. Current Medicinal Chemistry Anti-Cancer Agents 2:441-463 https://doi.org/10.2174/1568011023353930
  10. Goufo P, Trindade H (2014) Rice antioxidants: phenolic acids, flavonoids, anthocyanins, proanthocyanidins, tocopherols, tocotrienols, c-oryzanol, and phytic acid. Food Science and Nutrition 2:75-104 https://doi.org/10.1002/fsn3.86
  11. Grivetti LE, Shapiro HY (2009) Chocolate: History, culture, and heritage. (eds) J Wiley & Sons, Hoboken, NJ, pp. 3-15
  12. Handoyo T, Akagawa M, Morita M, Maeda T, Mitsunaga T (2008) Hypoallergenic characteristics of wheat flour produced by stepwise polishing. International Journal of Food Properties 1:243-252
  13. Katalinic V, Mozina SS, Generalic I, Skroza D, Ljubenkov I, Klancnik A (2013) Phenolic profile, antioxidant capacity, and antimicrobial activity of leaf extracts from Six Vitis vinifera L. varieties. International Journal of Food Properties 16:45-60 https://doi.org/10.1080/10942912.2010.526274
  14. Kim SG, Wang Y, Huh HH, Kim YC, Choi IS, Agrawal GK, Rakwal R, Kang KY, Kim ST (2011) Proteomics of ionic stresses in rice: An overview. J Plant Biotechnol 38:130-136 https://doi.org/10.5010/JPB.2011.38.2.130
  15. Kuo TM, VanMiddlesworth JF, Wolf WJ (1988) Content of raffinose oligosaccharides and sucrose in various plant seeds. Journal Agricultural and Food Chemistry 36:32-36 https://doi.org/10.1021/jf00079a008
  16. Kwon YS, Jeong MJ, Cha J, Jeong SW, Park SC, Shin SC, Chung WS, Bae H, Bae DW (2012) Comparative proteomic analysis of plant responses to sound waves in Arabidopsis. J Plant Biotechnol 39:261-272 https://doi.org/10.5010/JPB.2012.39.4.261
  17. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685 https://doi.org/10.1038/227680a0
  18. Larche M, Akdis CA, Valenta R (2006) Immunological mechanisms of allergen-specific immunotherapy. Nature Review Immunology 6:761-771 https://doi.org/10.1038/nri1934
  19. Miyake K, Morita R, Handoyo T (2004) Characterization of graded buckwheat flours and some properties of germinated 'Mancan' buckwheat grains. Fagopyrum 21:91-97
  20. Oko AO, Ubi BE, Efisue AA, Dambaba N (2012) Comparative analysis of the chemical nutrient composition of selected local and newly introduced rice varieties grown in ebonyi state of nigeria. International Journal of Agriculture and Forestry 2:16-23 https://doi.org/10.5923/j.ijaf.20120202.04
  21. Perr HA (2004) Novel foods to treat food allergy and gastrointestinal infection. Current Gastroenterology Reports 6:254-260 https://doi.org/10.1007/s11894-004-0016-3
  22. Peterbauer TR, Richter A (2001) Biochemistry and physiology of raffinose family oligosaccharides and galactosyl cyclitols in seeds. Seed Science Research 11:185-197
  23. Sanbongi C, Suzuki N, Sakane T (1997) Polyphenols in chocolate, which have antioxidant activity, modulate immune functions in humans in vitro. Cell Immune 177:129-136 https://doi.org/10.1006/cimm.1997.1109
  24. Shrime MG, Bauer SR, Mcdonald AC, Chowdhury NH, Coltart CEM, Ding EL (2011) Flavonoid-rich cocoa consumption affects multiple cardiovascular risk factors in a meta-analysis of short-term studies. Journal of Nutrition 141:1982-1988 https://doi.org/10.3945/jn.111.145482
  25. Taubert D, Berkels R, Roesen R, Klaus W (2003) Chocolate and blood pressure in elderly individuals with isolated systolic hypertension. Journal of The American Medical Association 290:1029-1030 https://doi.org/10.1001/jama.290.8.1029
  26. Yang F, Basu TK, Ooraikul B (2001) Studies on germination conditions and antioxidant contents of wheat grain. International Journal of Food Sciences and Nutrition 52:319-330 https://doi.org/10.1080/09637480120057567