Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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An efficient transformation method for a potato (Solanum tuberosum L. var. Atlantic)

  • Han, Eun-Hee (Dept. of Agronomy & Medicinal Plant Resources, College of Life Science, Gyeongnam National University of Science & Technology) ;
  • Goo, Young-Min (Sancheong Oriental Medicinal Herb Institute) ;
  • Lee, Min-Kyung (Dept. of Agronomy & Medicinal Plant Resources, College of Life Science, Gyeongnam National University of Science & Technology) ;
  • Lee, Shin-Woo (Dept. of Agronomy & Medicinal Plant Resources, College of Life Science, Gyeongnam National University of Science & Technology)
  • Received : 2015.03.06
  • Accepted : 2015.03.23
  • Published : 2015.06.30
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Abstract

We found that a long period of in vitro culture is a critical factor on the low transformation rate for a specific potato genotype, Solanum tuberosum L. var. Atlantic when phosphinothricin (PPT) was added to select putative transformants in a solid media. The fresh explants of the newly produced plants from a micro-tuber was able to increase the transformation rate significantly while the old explants prepared from a plant maintained for longer than 6 months in vitro by sub-culturing every 3 ~ 4 weeks resulted in a very low transformation frequency. However, Jowon cultivar was not so much influenced by the period of in vitro culture with high transformation rate (higher than 10.0%). Further research need to be explored for the reason why a particular potato genotype, Atlantic is more vulnerable than the Jowon cultivar during the regeneration stage resulting in the low transformation frequency.

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